Metabolite Changes Following Consumption Of Different Protein Drinks In Singapore Older Adults

NCT ID: NCT06956131

Last Updated: 2025-05-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

32 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-05-29

Study Completion Date

2025-12-31

Brief Summary

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The study aims to investigate the acute effects of different protein sources (i.e., casein, whey, soy) on metabolomic profiles (e.g., amino acids in the blood), along with other outcomes such as cognitive function, mood, and appetite. While the study does not directly aim to prevent, prognosticate, diagnose, or alleviate any disease, it may provide insights that could inform dietary recommendations and support disease prevention efforts in older adults.

Detailed Description

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This study will be a double-blind, randomised, placebo-controlled trial using a crossover study design. 25 older adults (aged 60 to 80) will be recruited and assigned to consume 4 different test drinks in a random order on 4 different visits. This study aims to investigate whether higher protein consumption of different sources will influence postprandial plasma and urinary metabolomic profiles, cognition, mood and appetite. The study will comprise 1 screening visit and 4 test visits separated separated by a minimum washout period of \>7 days

Conditions

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Aging Protein Metabolism

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

PREVENTION

Blinding Strategy

TRIPLE

Participants Investigators Outcome Assessors

Study Groups

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Casein Protein Drink

30 g protein equivalent of casein protein isolate dissolved in \~250 mL of water

Group Type EXPERIMENTAL

Casein Protein Isolate Drink

Intervention Type DIETARY_SUPPLEMENT

30 g protein equivalent of casein protein isolate dissolved in \~250 mL of water

Whey Protein Drink

30 g protein equivalent of whey protein isolate dissolved in \~250 mL of water

Group Type EXPERIMENTAL

Whey Protein Isolate Drink

Intervention Type DIETARY_SUPPLEMENT

30 g protein equivalent of whey protein isolate dissolved in \~250 mL of water

Soy Protein Drink

30 g protein equivalent of soy protein isolate dissolved in \~250 mL of water

Group Type EXPERIMENTAL

Soy Protein Isolate Drink

Intervention Type DIETARY_SUPPLEMENT

30 g protein equivalent of soy protein isolate dissolved in \~250 mL of water

Maltodextrin Drink

30 g carbohydrate equivalent of maltodextrin powder dissolved in \~250 mL of water

Group Type PLACEBO_COMPARATOR

Maltodextrin Drink

Intervention Type DIETARY_SUPPLEMENT

30 g carbohydrate equivalent of maltodextrin powder dissolved in \~250 mL of water

Interventions

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Casein Protein Isolate Drink

30 g protein equivalent of casein protein isolate dissolved in \~250 mL of water

Intervention Type DIETARY_SUPPLEMENT

Whey Protein Isolate Drink

30 g protein equivalent of whey protein isolate dissolved in \~250 mL of water

Intervention Type DIETARY_SUPPLEMENT

Soy Protein Isolate Drink

30 g protein equivalent of soy protein isolate dissolved in \~250 mL of water

Intervention Type DIETARY_SUPPLEMENT

Maltodextrin Drink

30 g carbohydrate equivalent of maltodextrin powder dissolved in \~250 mL of water

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* English-literate and able to give informed consent
* Adults 60 to 80 years old (inclusive)
* (If applicable) Postmenopausal
* BMI of 18·5 to 30 kg/m2 (inclusive)

Exclusion Criteria

* Currently participating in another clinical study that involves altering the regular diet and/or involves consuming a dietary supplement or investigated food product as an intervention
* Weight change \>3 kg in the past 3 months
* Exercising vigorously over the past 3 months (i.e., engaging in high-intensity exercises for more than 3 hours a week, where high-intensity exercises leave one out of breath and unable to talk or sing during the workout)
* Current smokers or have quit smoking for less than 5 years
* Drinking \>2 alcoholic drinks per day (i.e., one drink is defined as either 150 ml of wine, 340 ml of beer/cider or 45 ml of distilled spirit)
* If drinking ≤2 alcoholic drinks per day, not willing to stop consumption of alcoholic beverages two days before and on the day of the test visits (total 12 days)
* Not willing to stop consumption of caffeinated beverages 10 to 12 hours before and on the day of the test visits (total 4 days)
* Taking supplements containing omega-3, magnesium, gamma-aminobutyric acid (GABA), L-theanine, melatonin and/or 5-hydroxytryptophan for the past 3 months, and not willing to discontinue usage 3 months before and during the study duration
* Currently on a specialised diet (e.g., vegetarian, vegan, weight loss diet)

* Had serious food allergies in the past, or having intolerance, sensitivities or allergies to any of the following: milk/dairy products (e.g., casein, whey), starch/starch-derived products (e.g., rice, corn, wheat, potato), soy/soy-derived products, gluten, eggs, nuts, peanuts, fish and crustaceans.
* Disease or impairment of the kidney and/or liver
* Currently diagnosed with diabetes, sleep disorders, gastrointestinal issues (e.g., lactose intolerance, irritable bowel syndrome), gout and/or G6PD deficiency
* History of diseases known to affect the central nervous system (e.g., heart disease, stroke, migraine headaches, multiple sclerosis, Parkinson's disease, mental illnesses)
* Taking antibiotics 2 weeks prior to study participation
* Taking medication known to interfere with cognitive function and mental wellbeing (e.g., benzodiazepines, antidepressants, other central nervous agents)
* Taking anti-hypertensive or cholesterol-lowering drugs for less than 3 years unless medically declared by an official doctor to be in stable condition
* Poor peripheral venous access based on past experiences with blood drawing
Minimum Eligible Age

60 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Bezos Earth Fund

UNKNOWN

Sponsor Role collaborator

National University of Singapore

OTHER

Sponsor Role lead

Responsible Party

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Jung Eun Kim

Assistant Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Jung Eun Kim, Ph.D., R.D.

Role: PRINCIPAL_INVESTIGATOR

National University of Singapore, Department of Food Science and Technology

Locations

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NUS, Department of Food Science and Technology, 1 Science Drive 3, Block S13, #03-07

Singapore, , Singapore

Site Status RECRUITING

Countries

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Singapore

Central Contacts

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Nicole MY Wong, B.Sc.

Role: CONTACT

(+65) 8533 7360

Facility Contacts

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Nicole MY Wong, B.Sc.

Role: primary

(+65) 8533 7360

References

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Lanng SK, Oxfeldt M, Johansen FT, Risikesan J, Hansen M, Bertram HC. Acute changes in the metabolome following resistance exercise combined with intake of different protein sources (cricket, pea, whey). Metabolomics. 2023 Nov 24;19(12):98. doi: 10.1007/s11306-023-02064-0.

Reference Type BACKGROUND
PMID: 37999866 (View on PubMed)

Other Identifiers

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NUS-IRB-2025-19

Identifier Type: OTHER

Identifier Source: secondary_id

S31

Identifier Type: -

Identifier Source: org_study_id

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