Eccentric Cycling Exercise on Mitochondrial Function of Lymphocyte

NCT ID: NCT06576804

Last Updated: 2024-08-29

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

33 participants

Study Classification

INTERVENTIONAL

Study Start Date

2021-01-01

Study Completion Date

2022-08-01

Brief Summary

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Eccentric cycling exercise (ECE) features lower metabolic demand and higher improvement of muscle strength compared to traditional concentric cycling exercise (CCE). Mitochondria can regulate energy metabolism and adaptive immune quality in T lymphocytes. However, the effects of ECE on mitochondrial functions in T-lymphocytes have not yet been established.

Method: A total of 33 healthy sedentary males were randomized and divided into ECE (n=11), CCE (n=11), and control groups (n=11). These subjects progressively performed CCE or ECE from 60% to 80% maximal workload on a bicycle ergometer for 40 min/day, 5 days/week for 6 weeks. A graded exercise testing and an isokinetic strength test were conducted to evaluate cardiopulmonary fitness and muscle strength, respectively. Phenotypes and mitochondrial respiratory capacity in T lymphocyte were analyzed using flow cytometry and high-resolution respirometer, respectively.

Detailed Description

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Eccentric exercise training (EET) increases physical performance while having lower metabolic demand than concentric exercise training (CET).

Mitochondria of lymphocytes are essential for cell proliferation, death, and differentiation, and play a critical role in establishing lymphocyte phenotypes and their functions.

Whether EET influences lymphocyte bioenergetic efficiency remains unclear. The study was to investigate the effects of ECE and CCE regimens on adaptive immune functions and mitochondrial bioenergetics of T lymphocytes in sedentary males.

Conditions

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Eccentric Exercise Training

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

SINGLE

Outcome Assessors

Study Groups

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Eccentric cycling training

Moderate to high intensity cycling training in eccentric type

Group Type EXPERIMENTAL

Eccentric cycling training

Intervention Type BEHAVIORAL

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Concentric cycling training

Moderate to high intensity cycling training in concentric type (intensity matched the eccentric training)

Group Type EXPERIMENTAL

Concentric cycling training

Intervention Type BEHAVIORAL

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Control group

without receiving any exercise training

Group Type NO_INTERVENTION

No interventions assigned to this group

Interventions

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Eccentric cycling training

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Intervention Type BEHAVIORAL

Concentric cycling training

Performed exercise training 5 days a week for 6 weeks on an eccentric ergometer.

Each training session: 5 min at 30% of maximal workload (Wmax) for warmed up and cold down and 30 min the main training phase.

Intensity: Firstly, set at 60% Wmax, and progressively increased 5% per week

Intervention Type BEHAVIORAL

Eligibility Criteria

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Inclusion Criteria

1. Sedentary lifestyle.
2. Nonsmokers, nonusers of medications/vitamins.
3. No any cardiopulmonary/hematological risks.

Exclusion Criteria

1. Have regular exercise habits (i.e., exercise frequency once per week, duration \>20 min).
2. Have retabolic or musculoskeletal disease.
Minimum Eligible Age

20 Years

Maximum Eligible Age

30 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Chang Gung Memorial Hospital

OTHER

Sponsor Role lead

Responsible Party

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Jong-Shyan Wang

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Chang Gung University

Taoyuan District, , Taiwan

Site Status

Countries

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Taiwan

References

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LaStayo PC, Ewy GA, Pierotti DD, Johns RK, Lindstedt S. The positive effects of negative work: increased muscle strength and decreased fall risk in a frail elderly population. J Gerontol A Biol Sci Med Sci. 2003 May;58(5):M419-24. doi: 10.1093/gerona/58.5.m419.

Reference Type RESULT
PMID: 12730250 (View on PubMed)

Hody S, Croisier JL, Bury T, Rogister B, Leprince P. Eccentric Muscle Contractions: Risks and Benefits. Front Physiol. 2019 May 3;10:536. doi: 10.3389/fphys.2019.00536. eCollection 2019.

Reference Type RESULT
PMID: 31130877 (View on PubMed)

Touron J, Costes F, Coudeyre E, Perrault H, Richard R. Aerobic Metabolic Adaptations in Endurance Eccentric Exercise and Training: From Whole Body to Mitochondria. Front Physiol. 2021 Jan 27;11:596351. doi: 10.3389/fphys.2020.596351. eCollection 2020.

Reference Type RESULT
PMID: 33584331 (View on PubMed)

Tsai HH, Chang SC, Chou CH, Weng TP, Hsu CC, Wang JS. Exercise Training Alleviates Hypoxia-induced Mitochondrial Dysfunction in the Lymphocytes of Sedentary Males. Sci Rep. 2016 Oct 12;6:35170. doi: 10.1038/srep35170.

Reference Type RESULT
PMID: 27731374 (View on PubMed)

Other Identifiers

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202100004A3

Identifier Type: -

Identifier Source: org_study_id

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