Time-course of Mitochondrial Biogenic Gene and Protein Expression in Exercised Human Skeletal Muscle

NCT ID: NCT03975777

Last Updated: 2020-03-04

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

NA

Total Enrollment

10 participants

Study Classification

INTERVENTIONAL

Study Start Date

2019-06-01

Study Completion Date

2020-04-30

Brief Summary

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This study examines the impact of exercise intensity on the 12-hour time-course of mitochondrial biogenic gene and protein expression in human skeletal muscle. Briefly, participants will perform two experimental sessions involving an acute bout of work-matched low- or high-intensity interval cycling exercise in a randomized crossover fashion, two weeks apart. Skeletal muscle biopsies will be obtained from the vastus lateralis (3 from each leg) before and 1, 3, 6, 9, and 12 hours post-exercise. Changes in the expression of various transcription factors and mitochondrial proteins will be examined at the mRNA and protein level to determine the time-course of changes in these factors differs between exercise protocols of different intensities.

Detailed Description

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Conditions

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Mitochondrial Biogenesis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

DOUBLE

Investigators Outcome Assessors

Study Groups

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low-intensity exercise - high-intensity exercise

low-intensity exercise session followed by a high-intensity exercise session separated by a minimum of 14 days

Group Type EXPERIMENTAL

LO

Intervention Type OTHER

Participants will perform an acute bout of low-intensity cycling (11 x 1 min intervals at \~73% of peak aerobic work rate interspersed with 1 min recovery periods)

HI

Intervention Type OTHER

Participants will perform an acute bout of high-intensity cycling (8 x 1 min intervals at \~100% of peak aerobic work rate interspersed with 1 min recovery periods)

high-intensity exercise - low-intensity exercise

high-intensity exercise session followed by a low-intensity exercise session separated by a minimum of 14 days

Group Type EXPERIMENTAL

LO

Intervention Type OTHER

Participants will perform an acute bout of low-intensity cycling (11 x 1 min intervals at \~73% of peak aerobic work rate interspersed with 1 min recovery periods)

HI

Intervention Type OTHER

Participants will perform an acute bout of high-intensity cycling (8 x 1 min intervals at \~100% of peak aerobic work rate interspersed with 1 min recovery periods)

Interventions

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LO

Participants will perform an acute bout of low-intensity cycling (11 x 1 min intervals at \~73% of peak aerobic work rate interspersed with 1 min recovery periods)

Intervention Type OTHER

HI

Participants will perform an acute bout of high-intensity cycling (8 x 1 min intervals at \~100% of peak aerobic work rate interspersed with 1 min recovery periods)

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* recreationally active (no more than three hours of structured weekly physical activity)
* not currently involved in a systematic training program for improving cardiorespiratory fitness, muscular strength, and/or sport-specific skills
* body mass index \< 30 kg/m2

Exclusion Criteria

* presence of cardiometabolic disease
* taking regular prescription medication for the treatment of cardiometabolic disease
* current cigarette smoker
Minimum Eligible Age

18 Years

Maximum Eligible Age

30 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Brendon Gurd, PhD

OTHER

Sponsor Role lead

Responsible Party

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Brendon Gurd, PhD

Associate Professor

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Brendon J. Gurd, PhD

Role: PRINCIPAL_INVESTIGATOR

School of Kinesiology and Health Studies

Locations

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School of Kinesiology and Health Studies

Kingston, Ontario, Canada

Site Status RECRUITING

Countries

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Canada

Central Contacts

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Hashim Islam, MSc

Role: CONTACT

4037954125

Facility Contacts

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Hashim Islam, MSc

Role: primary

4037954125

Other Identifiers

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QMPL-TC-19

Identifier Type: -

Identifier Source: org_study_id

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