Markers of Carnitine Acetyltransferase (CrAT) Protein Activity and Carnitine Availability

NCT ID: NCT03697928

Last Updated: 2020-10-19

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 13 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2019-03-12
• Study Completion Date: 2020-02-01

Brief Summary

This study aims to measure skeletal muscle metabolism in vivo during exercise using the non-invasive Magnetic Resonance Spectroscopy (MRS) technique. Specifically, phosphocreatine (PCr) kinetic and Acetylcarnitine levels will be determined at resting, during exercise and during the recovery post exercise. The target population is adult healthy men, with a wide range of maximal physical capacity.

Detailed Description

Skeletal muscle mitochondrial activation during the transition from resting metabolism to exercise may modulated by CrAT activity. Furthermore, dynamic changes in acetylcarnitine during the transition from exercise to resting metabolism (acetylcarnitine off-kinetic) may also reflect the action of CrAT protein. To date, the only possibility to get information on CrAT activity is by taking a muscle biopsy and perform enzyme activity assays ex vivo. By dynamic MRS scanning, it might be possible to deduce information that reflects CrAT activity, thereby making the biopsy sampling unnecessary.

Conditions

Metabolism and Nutrition Disorder; Metabolic Disturbance

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: CROSS_SECTIONAL

Study Groups

CrAT subjects

13 healthy adult men, with a wide range of maximal aerobic capacity will be included.

Subjects will be classified as trained, physical active and untrained according to their VO2max.

Subject will perform one-legged knee extension and flexion exercise inside the MRS scanner and cycling outside the scanner

Exercise metabolism

Intervention Type: OTHER

Participants will perform a series of exercises inside and outside the MRS/MRI scanner.

Interventions

Exercise metabolism

Participants will perform a series of exercises inside and outside the MRS/MRI scanner.

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Males, Generally healthy
• Age 18-40 years old
• BMI 18 - 25 kg/m2
• No medication use that interfere with the aims of the study
• Stable level of daily physical activity or training for at least 6 months

Exclusion Criteria

• Females
• Regular smokers, drug abuse
• Participation in other studies
• Weight gain/loss > 3 kg in the last 6 months
• Contraindications for MRS scans:
• Vegetarian eating behavior

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 40 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

Maastricht University

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Vera Schrauwen-Hinderling, Phd

Role: PRINCIPAL_INVESTIGATOR

Maastricht University

Locations

Maastricht University

Maastricht, Limburg, Netherlands

Countries

Netherlands

References

Mancilla R, Pava-Mejia D, van Polanen N, de Wit V, Bergman M, Grevendonk L, Jorgensen J, Kornips E, Hoeks J, Hesselink MKC, Schrauwen-Hinderling VB. Invasive and noninvasive markers of human skeletal muscle mitochondrial function. Physiol Rep. 2023 Jun;11(12):e15734. doi: 10.14814/phy2.15734.

Reference Type: DERIVED

PMID: 37340318 (View on PubMed)

Other Identifiers

NL63005.068.18

Identifier Type: -

Identifier Source: org_study_id