Immunometabolism of Exercise

NCT ID: NCT07188389

Last Updated: 2025-09-23

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

102 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-03-01

Study Completion Date

2025-04-03

Brief Summary

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This study aims to examine the effects of acute aerobic exercise and cardiorespiratory fitness on cellular metabolism of CD8+ T cells and regulatory T cells (Tregs) found in the peripheral blood of humans. In addition, the study will investigate whether the effects of exercise differ based on exercise intensity, as well as whether the effects of exercise and fitness differ between subpopulations of CD8+ T cells and Tregs. Finally, the study aims to examine whether exercise- and fitness-induced changes in cell metabolism relate to changes in cell function.

Detailed Description

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Conditions

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Cardiorespiratory Fitness and Acute Exercise

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

SINGLE

Outcome Assessors

Study Groups

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Acute effects: Moderate Intensity Exercise

Participants with a moderate fitness level (determined by VO2max value recorded during graded exercise test) will cycle 30minutes exercise below ventilatory threshold. Outcome measures will be compared within-subject across blood sampled before exercise, immediately after exercise, and 2h post exercise. The order of the exercise sessions (above vs below threshold) will be randomized.

Group Type EXPERIMENTAL

Acute exercise-moderate intensity

Intervention Type BEHAVIORAL

30 minutes of cycling exercise at moderate (90% of first ventilatory threshold)

Acute effects: Heavy intensity exercise

Participants with a moderate fitness level (determined by VO2max value recorded during graded exercise test) will bicycle 30 minutes above ventilatory threshold. Outcome measures will be compared within-subject across blood sampled before exercise, immediately after exercise, and 2h post exercise. The order of the exercise sessions (above vs below threshold) will be randomized.

Group Type EXPERIMENTAL

Acute effects-heavy intensity

Intervention Type BEHAVIORAL

Participants will cycle 30 minutes at heavy (110% of first ventilatory threshold) intensity

Interventions

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Acute exercise-moderate intensity

30 minutes of cycling exercise at moderate (90% of first ventilatory threshold)

Intervention Type BEHAVIORAL

Acute effects-heavy intensity

Participants will cycle 30 minutes at heavy (110% of first ventilatory threshold) intensity

Intervention Type BEHAVIORAL

Eligibility Criteria

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Inclusion Criteria

* Men and women, aged 20-40 years
* individuals presenting with average (40th-60th percentile) cardiorespiratory fitness (i.e., VO2max/VO2peak) for their age and sex will be included in acute exercise experiments; individuals with VO2max/VO2peak value \>20ml/kg/min will be included in cardiorespiratory fitness experiments
* individuals categorized as 'low risk' for a cardiovascular event/heart disease when screened by American College of Sports Medicine (ACSM) criteria

Exclusion Criteria

* adults under the age of 20 and over the age of 40
* Individuals with contraindications to exercise testing will be excluded
* Individuals with musculoskeletal impairments preventing them from exercising on a cycle ergometer
* Individuals with disease history known to impact the immune system and/or systemic metabolism will be excluded, including autoimmune diseases, HIV, Hepatitis, diabetes, cancer (completed treatment within \<6 months prior), hyper- or hypothyroidism
* Pregnancy
* Individuals using medications known to impact the cardiopulmonary and/or immune response to exercise with be excluded, including those using medication to control blood pressure (e.g., beta-blockers), daily steroidal and nonsteroidal anti-inflammatories (except daily aspirin), prescription allergy medication, ADHD medication (Ritalin or Adderall), thyroid medication (e.g., Levothyroxine), and chemotherapy (including treatments within the last 6 months).
* Individuals habitually using tobacco products within the last 6 months or with excessive alcohol intake (\>10 drinks/week)
Minimum Eligible Age

20 Years

Maximum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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University of Houston

OTHER

Sponsor Role lead

Responsible Party

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Emily C Lavoy

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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University of Houston

Houston, Texas, United States

Site Status

Countries

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United States

Other Identifiers

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STUDY00004272

Identifier Type: -

Identifier Source: org_study_id

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