T Cell Responses to Various Levels of Exercise Stress

NCT ID: NCT06638684

Last Updated: 2024-10-15

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 16 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2015-02-20
• Study Completion Date: 2016-05-12

Brief Summary

The objective of this project is to quantify the immunoendocrine response to acute stress events combining both physical and psychological challenges. This work will serve as a pilot project examining differential stress induction in response to exercise. Participants will have an instructor with prior military training lead a two-hour training session for 10 recreationally fit male volunteers. You will be randomly assigned to a high-intensity training group to participate in the "military style" training and a low-intensity training group who will participate in a low-intensity cardiovascular training protocol. Blood samples will be collected from you prior to and following the exercise session and these samples will be analyzed for endocrine measures as well as markers of immune function to include chemokines and cytokines.

Conditions

Healthy

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Moderate intensity exercise

Subjects will participate in a 100 min bout of exercise led by an exercise physiologist doing the same exercises as the high-intensity group, but at a lower effort level. Exercise consists of of a mix of running activities (≤ 400 m); body weight exercises, such as burpees and lunges; and exercises, such as crunches and squats utilizing 20.4 kg weight plates, but performed at a pace allowing for in-session recovery. Scheduled 5 min rests will be included at 30, 60, and 85 min and MI subjects will be similarly encouraged to consume water and/or the carbohydrate/electrolyte drink. The researchers will obtain a blood sample from you prior to and immediately following the exercise, as well as one, four and six hours post-exercise.

Group Type: ACTIVE_COMPARATOR

Exercise

Intervention Type: OTHER

Subjects in the two groups preformed 90-100 minutes of exercise at different intensities.

High intensity exercise

Subjects in this group will participate in an exercise session that is representative of a training event experienced by cadets preparing for military competitions. All exercises will consist of a mix of running activities (≤ 400 m); body weight exercises, such as burpees and lunges; and exercises, such as crunches and squats utilizing 20.4 kg weight plates; with rapidly occurring exercise transitions to prevent recovery. The exercise bout will last a total of 100 min. Scheduled 5 min rests will be inserted at 30, 60, and 85 min. During the scheduled rest periods, subjects will be encouraged to consume water and/or the carbohydrate/electrolyte drink. The researchers will obtain a blood sample from you prior to and immediately following the exercise, as well as one, four and six hours post-exercise.

Group Type: EXPERIMENTAL

Exercise

Intervention Type: OTHER

Subjects in the two groups preformed 90-100 minutes of exercise at different intensities.

Interventions

Exercise

Subjects in the two groups preformed 90-100 minutes of exercise at different intensities.

Intervention Type: OTHER

Other Intervention Names

Moderate-intensity; High-intensity

Eligibility Criteria

Inclusion Criteria

• Recreationally active
• Between the ages of 18-40,
• Healthy
• Non-obese (BMI <28 kg/m2)
• Non-smoking
• Free of metabolic or cardiovascular diseases.

Exclusion Criteria

• Unhealed Fractures
• Thrombophlebitis (blood clots)
• Recent Surgery (within the last three months)
• Recent Uncontrolled Bruising
• Osteomyelitis (acute or chronic bone infection)
• Myositis Ossificans (hardened scarring in muscle tissue of the thigh).

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 35 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

University of Kansas

OTHER

Sponsor Role: lead

Responsible Party

Philip Gallagher

Professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

University of Kansas

Lawrence, Kansas, United States

Countries

United States

References

Siedlik J, Deckert J, Dunbar A, Bhatta A, Gigliotti N, Chan M, Benedict S, Bubak M, Vardiman J, Gallagher P. Acute high-intensity exercise enhances T cell proliferation compared to moderate-intensity exercise. Appl Physiol Nutr Metab. 2025 Jan 1;50:1-12. doi: 10.1139/apnm-2024-0420.

Reference Type: DERIVED

PMID: 39947139 (View on PubMed)

Other Identifiers

ONR N000141410202

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

STUDY00001969

Identifier Type: -

Identifier Source: org_study_id