Resistance Exercise and Hydrolyzed Collagen Supplementation

NCT ID: NCT05932771

Last Updated: 2023-07-06

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 21 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2019-01-01
• Study Completion Date: 2023-04-30

Brief Summary

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Detailed Description

This study aimed to investigate the effect of different doses of hydrolyzed collagen (HC) with resistance exercise (RE) on whole body collagen synthesis in healthy young and older males and females.

Healthy young and older males and females ingested 0 grams, 15 grams or 30 grams HC with 50 milligrams vitamin C 1h prior to performing four sets' barbell back-squat RE at 10-repetition maximum load, after which they rested for six hours. Blood samples were collected throughout each trial to analyse procollagen type Ⅰ N-terminal propeptide (PⅠNP, a biomarker of collagen synthesis) and β-isomerized C-terminal telopeptide of type I collagen (β-CTX, a biomarker of collagen breakdown) concentration, and the concentration of 18 amino acids that constitute collagen.

This is the first study to investigate the combined effect of different doses HC with high-intensity RE on whole body collagen synthesis in healthy young and older men and women. If 30 grams HC intake with RE does augment collagen synthesis more than RE alone, this suggests that long-term HC intake with chronic RE would be beneficial for tendon health, because collagen is the most abundant protein in tendon.

Conditions

Diet, Food, and Nutrition

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: TRIPLE

Study Groups

1.Trial for young male participants: Consuming hydrolyzed collagen (HC) with reistance exercise

Young male participants consumed one of three different HC doses (0 grams, 15 grams, or 30 grams) with 4 sets of 10 repetitions of barbell back squat exercise at 10-repetition maximum load in a random order and a seven-day wash-out period interspersed between each trial.

Group Type: EXPERIMENTAL

Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise

Intervention Type: DIETARY_SUPPLEMENT

Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula.

All trials were performed at the same time of day (08:00 - 15:00).

Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses.

The details of nutritoinal supplements used are as follows:

Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

2.Trial for young female participant: Consuming hydrolyzed collagen (HC) with reistance exercise

The intervention procedure is exactly same as Arm 1 except for the number of visits. Young female participant was asked to visit the laboratory on four occasions during two consecutive months. Therefore, there were two trials in each month, where female participants' estrogen level was lower (i.e., onset of menses) or higher (i.e., ovulation). Dates for the trials were determined based on self-report of onset of menses and previous menstrual cycle length.

Group Type: EXPERIMENTAL

Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise

Intervention Type: DIETARY_SUPPLEMENT

Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula.

All trials were performed at the same time of day (08:00 - 15:00).

Different doses of HC (0 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams maltodextrin was used in 0 grams HC. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses.

The details of nutritoinal supplements used are as follows:

Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

3.Trial for older male participants: Consuming hydrolyzed collagen (HC) with reistance exercise

The age range of older male participants was 40 - 65 years. The intervention procedure is exactly same as Arm 1

Group Type: EXPERIMENTAL

Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise

Intervention Type: DIETARY_SUPPLEMENT

Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula.

All trials were performed at the same time of day (08:00 - 15:00).

Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses.

The details of nutritoinal supplements used are as follows:

Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

4.Trial for older female participants: Consuming hydrolyzed collagen (HC) with reistance exercise

The age range of older female participants was 40 - 65 years. The intervention procedure is exactly same as Arm 2

Group Type: EXPERIMENTAL

Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise

Intervention Type: DIETARY_SUPPLEMENT

Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula.

All trials were performed at the same time of day (08:00 - 15:00).

Different doses of HC (0 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams maltodextrin was used in 0 grams HC. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses.

The details of nutritoinal supplements used are as follows:

Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

Interventions

Experimental: Consumption of three different doses of hydrolyzed collagen (HC) with reistance exercise

Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula.

All trials were performed at the same time of day (08:00 - 15:00).

Different doses of HC (0 grams, 15 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams and 15.4 grams maltodextrin was used in 0 grams HC and 15 grams HC respectively. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses.

The details of nutritoinal supplements used are as follows:

Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

Intervention Type: DIETARY_SUPPLEMENT

Experimental: Consumption of two different doses of hydrolyzed collagen (HC) with reistance exercise

Each trial lasts for seven hours and while participants consumed HC, performed resistance exercise and rested, 10 x 5 mL blood samples were collected from a superficial forearm vein using a cannula.

All trials were performed at the same time of day (08:00 - 15:00).

Different doses of HC (0 grams and 30 grams) and 50 milligrams vitamin C were dissolved with 250 milliliter water in an opaque bottle. To match calories of 30 grams HC, 34.1 grams maltodextrin was used in 0 grams HC. Also to mask any potential difference in HC doses, 4 grams non-caloric sweetener was used in all HC doses.

The details of nutritoinal supplements used are as follows:

Hydrolysed collagen (Myprotein, Cheshire, UK), Vitamin C powder (Holland and Barrett Retail Limited, Warwickshire, UK), Maltodextrin (Myprotein, Cheshire, UK), and Non-caloric sweetener (Truvia®, SilverSpoon, London, UK)

Intervention Type: DIETARY_SUPPLEMENT

Other Intervention Names

Resistance exercise and collagen dose-response for male participants; Resistance exercise and collagen dose-response for female participants

Eligibility Criteria

Inclusion Criteria

• At least 12 months experienced in resistance exercise (regular back squat experience)
• Free from musculoskeletal injury
• Nullipara (a woman who has never given birth) (only for young female participatns)

Exclusion Criteria

• Having a history of patellar tendon pathology
• Vegan
• Consumed nutritional supplements or medication purported to have beneficial effects on muscle-tendon properties (e.g. antioxidants, protein, etc.)
• Had a sustained a lower limb injury in the previous six months
• Smoker/vaper
• <18 or >40 years old (only for young participants)
• <40 ro >65 years old (only for older participants)
• Having an irregular menstrual cycle (only for young female participants)

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 65 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

University of East Anglia

OTHER

Sponsor Role: collaborator

Rob Erskine

OTHER

Sponsor Role: lead

Responsible Party

Rob Erskine

Reader in Neuromuscular Physiology

Responsibility Role: SPONSOR_INVESTIGATOR

Principal Investigators

Robert M. Erskine, PhD

Role: STUDY_DIRECTOR

Liverpool John Moores University

Joonsung Lee, MSc

Role: PRINCIPAL_INVESTIGATOR

Liverpool John Moores University

Christopher Nulty

Role: PRINCIPAL_INVESTIGATOR

Liverpool John Moores University

Locations

Dr Rob Erskine

Liverpool, , United Kingdom

Countries

United Kingdom

Provided Documents

Document Type: Study Protocol, Statistical Analysis Plan, and Informed Consent Form

View Document

Other Identifiers

18/SPS/059

Identifier Type: -

Identifier Source: org_study_id