Practicality of Intermittent Fasting and Its Effect on Markers of Aging and Oxidative Stress

NCT ID: NCT02132091

Last Updated: 2015-12-14

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

37 participants

Study Classification

INTERVENTIONAL

Study Start Date

2011-03-31

Study Completion Date

2011-11-30

Brief Summary

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Healthy volunteers will be recruited to participate in a ten-week double-blinded crossover trial. The trial will consist of two, three-week periods of intermittent fasting, where subjects receive either antioxidant supplementation or placebo, the ordering of which will be randomly determined. A one-week preconditioning will precede each invention period, and a two week "wash-out" period will follow the first intervention period. Serum-based assays will be performed to assess levels of reactive oxidant species, antioxidant genes, sirtuins, and markers of mitochondrial biogenesis and aging.

The investigators hypothesize that an intermittent fasting diet in healthy young volunteers will improve these markers of cellular aging and that these beneficial effects will be abrogated by the supplementation of antioxidants. This study is a proof-of-principle study that will shed light on the mechanism and effects of IF as an anti-aging dietary intervention in the absence of weight loss. It will inform the design of dietary interventions that are both effective in improving markers of aging and feasible for patients to practice on a long-term basis.

Detailed Description

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Conditions

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Aging Metabolism

Keywords

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Sirtuins Calorie Restriction Intermittent Fasting Antioxidant Aging Metabolism Mitochondria Oxidative Stress

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Intermittent Fasting

Intermittent Fasting

Group Type EXPERIMENTAL

Intermittent Fasting

Intervention Type OTHER

The intermittent fasting paradigm used in this study will require participants to alternate between days of feasting (175% of normal caloric intake) and fasting ( 25% of normal caloric intake). Food will be provided by University of Florida Clinical Research Center with macronutrient composition prepared according to the 2010 Dietary Guidelines for Americans.

Intermittent Fasting + Antioxidants

Intermittent Fasting; 400 IU Vitamin E; 1000 mg Vitamin C

Group Type EXPERIMENTAL

Intermittent Fasting

Intervention Type OTHER

The intermittent fasting paradigm used in this study will require participants to alternate between days of feasting (175% of normal caloric intake) and fasting ( 25% of normal caloric intake). Food will be provided by University of Florida Clinical Research Center with macronutrient composition prepared according to the 2010 Dietary Guidelines for Americans.

400 IU Vitamin E

Intervention Type DIETARY_SUPPLEMENT

once each day in morning; oral pill form

1000 mg Vitamin C

Intervention Type DIETARY_SUPPLEMENT

500mg twice each day; morning and evening; oral pill form

Interventions

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Intermittent Fasting

The intermittent fasting paradigm used in this study will require participants to alternate between days of feasting (175% of normal caloric intake) and fasting ( 25% of normal caloric intake). Food will be provided by University of Florida Clinical Research Center with macronutrient composition prepared according to the 2010 Dietary Guidelines for Americans.

Intervention Type OTHER

400 IU Vitamin E

once each day in morning; oral pill form

Intervention Type DIETARY_SUPPLEMENT

1000 mg Vitamin C

500mg twice each day; morning and evening; oral pill form

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Body mass index in the range of 20.0-30.0 kg/m2;
* Age between 19 and 30;
* Stable weight (change \<±10%) for 3 months immediately prior to the study
* No history of metabolic disorders (e.g. non-diabetic), cardiovascular disease, or thyroid dysfunction
* No past or present eating disorders
* No acute or chronic inflammatory disorder
* No more than moderate physical activity (i.e.,\<3 hour/week of light exercise sessions for the past 3 months)
* No current medications to regulate blood sugar or lipids
* Not donated blood within 56 days of study start date
* No food allergies
* No dietary restrictions (e.g. vegetarianism and vegan)
* No heavy drinking (more than 15 drinks/week)
* No use of tobacco or recreational drugs within past 3 months
* Access to a microwave or stove
* Access to refrigeration

Exclusion Criteria

* Inflexibility of schedule such that subject cannot attend blood draw appointments
* Unwillingness to pick-up and eat only study-provided food during the 8 weeks in which it is provided and for unwillingness to abstain from travel (\>48hrs) during these same 8 weeks of the trial (travel is permitted during wash-out period)
* Unwillingness to abstain from tobacco, alcohol, recreational drugs, resveratrol or antioxidant supplements (other than study-provided) for the duration of study
* Women who are pregnant, breast-feeding or trying to become pregnant
Minimum Eligible Age

19 Years

Maximum Eligible Age

30 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National Institutes of Health (NIH)

NIH

Sponsor Role collaborator

National Institute on Aging (NIA)

NIH

Sponsor Role collaborator

University of Florida

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Mark L Brantly, MD

Role: PRINCIPAL_INVESTIGATOR

University of Florida

Locations

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University of Florida

Gainesville, Florida, United States

Site Status

Countries

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United States

References

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Wegman MP, Guo MH, Bennion DM, Shankar MN, Chrzanowski SM, Goldberg LA, Xu J, Williams TA, Lu X, Hsu SI, Anton SD, Leeuwenburgh C, Brantly ML. Practicality of intermittent fasting in humans and its effect on oxidative stress and genes related to aging and metabolism. Rejuvenation Res. 2015 Apr;18(2):162-72. doi: 10.1089/rej.2014.1624.

Reference Type DERIVED
PMID: 25546413 (View on PubMed)

Other Identifiers

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TL1TR000066

Identifier Type: NIH

Identifier Source: secondary_id

View Link

UL1TR000064

Identifier Type: NIH

Identifier Source: secondary_id

View Link

1P30AG028740

Identifier Type: NIH

Identifier Source: secondary_id

View Link

IRB772011

Identifier Type: -

Identifier Source: org_study_id