Human Sperm Markers of Cryodamage Resistance

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

40 participants

Study Classification

OBSERVATIONAL

Study Start Date

2015-02-12

Study Completion Date

2019-12-12

Brief Summary

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Sperm freezing has been employed for decades for male fertility preservation in cases of foreseeable or unexpected loss of fertility to guarantee future paternity, and also as a complement of assisted reproduction techniques.

Sperm quality after thawing is highly variable, even among consecutive samples from the same individual, with mean survival rates around 40%. To date, the molecular basis of the adequate resistance or intolerance to freezing/thawing protocols is unknown, and its knowledge can lead to improvement in the selection of the samples to be frozen and also in the adequate supplement of cryopreservation media. Microarray analysis provides a powerful tool to address the molecular explanation beyond this behaviour, yielding results about comparative messenger ribonucleic acid (mRNA)expression under the two different biological conditions: optimal and suboptimal survival.

Then, the aim of the investigators' study is to determine the genomic profile of sperm samples depending on their survival resistance to cryopreservation, to determine genes involved in cryodamage sensitivity.

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Detailed Description

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Nested cases and controls design, with donor sperm samples frozen under conventional protocols, categorized depending on their good (GSR, n=20) (less than 20% motility decrease) or bad (BSR, n=20) (more than 20% motility decrease) survival rates. Sperm mRNA was extracted using Trizol protocol, suspended in diethylpyrocarbonate (DEPC)-treated water and frozen at -80 degrees until the microarray experiments were performed. RNAs were analyzed on Agilent Bioanalyzer 2100. Samples were pooled in 4 (5 samples/pool and 4 pools/group). Finally, 8 Agilent One colour Whole genome microarray (44K) were performed with pooled samples, 4 microarrays per group.

The results will be evaluate to detect those genes differentially expressed.

Conditions

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Fertility

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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High cryoresistance

High resistance to sperm cryopreservation (decreased sperm motility \<20%)

Sperm cryopreservation

Intervention Type OTHER

Sperm from the different groups are cryopreserved to evaluate their resistance to preservation.

Low cryoresistance

Low resistance to sperm cryopreservation (decreased sperm motility \>20%)

Sperm cryopreservation

Intervention Type OTHER

Sperm from the different groups are cryopreserved to evaluate their resistance to preservation.

Interventions

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Sperm cryopreservation

Sperm from the different groups are cryopreserved to evaluate their resistance to preservation.

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

The criteria used for the inclusion of donors in the sperm bank will be considered:

* males from 18 to 35 years.
* good physical and psychological conditions and no hereditary diseases
* seminal parameters: concentration \> 40 million sperm / ml; percentage of progressive motility\> 50% percentage of normal forms\> 4%.
* For pregnancy rates after artificial insemination, only women\< 40 years without tubal pathologies and artificial insemination indications will be included.