Hypotaurine Supplementation Benefits in Cryopreservation

NCT ID: NCT04011813

Last Updated: 2019-07-09

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 33 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2014-05-01
• Study Completion Date: 2019-05-01

Brief Summary

Although it is widely used, slow freezing can induce strong functional and nuclear spermatic alterations reducing the chances of pregnancy. The study objective is to determinate the effects of the combination of hypotaurine supplementation and spermatozoa selection by Density Gradient Centrifugation (DGC) on human sperm functions and DNA quality during a freezing-thawing cycle.

Detailed Description

This prospective study was performed on surplus semen after a density gradient centrifugation-frozen-thawing cycle. Samples were obtained from men undergoing routine semen analysis at the Center for Reproductive Medicine. Spermatozoa were selected by density gradient centrifugation, washed and frozen using a programmable device. Each step was performed in parallel with (H+ arm) or without (H- arm) 50mM hypotaurine supplementation. After thawing, investigator team compared for both conditions the total and progressive mobility, vitality, integrity of the acrosome, markers of Protein Kinase A (PKA) dependent capacitation intracellular signaling pathway and nuclear quality by measuring chromatin packaging, DNA fragmentation and oxidation and vacuoles presence in the spermatozoa head.

Conditions

Cryopreservation; Human Spermatozoa Parameters; Hypotaurine Supplementation; Spermatozoa DNA Alterations

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

Control arm, "H-"

semen treated without hypotaurine supplementation in density gradient centrifugation, washing and cryopreservation media

No interventions assigned to this group

Experimental arm, "H+"

semen treated with a 50mM hypotaurine supplementation in density gradient centrifugation, washing and cryopreservation media

Hypotaurine : antioxidant and osmoregulator

Intervention Type: OTHER

Hypotaurine has protective effects on sperm motility, capacitation and acrosome reaction and reduces apoptotic markers.

Hypotaurine (50mM) was added in density gradient centrifugation, washing and cryopreservation media washing and cryopreservation media before spermatozoa freezing

Interventions

Hypotaurine : antioxidant and osmoregulator

Hypotaurine has protective effects on sperm motility, capacitation and acrosome reaction and reduces apoptotic markers.

Hypotaurine (50mM) was added in density gradient centrifugation, washing and cryopreservation media washing and cryopreservation media before spermatozoa freezing

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• None

Exclusion Criteria

• None

• Minimum Eligible Age: 18 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: No

Sponsors

Université d'Auvergne, Institut National de la Santéet de la Recherche Médicale

UNKNOWN

Sponsor Role: collaborator

Université d'Auvergne, Centre National de la Recherche Scientifique

UNKNOWN

Sponsor Role: collaborator

University Hospital, Clermont-Ferrand, Hematology laboratory

UNKNOWN

Sponsor Role: collaborator

University Hospital, Clermont-Ferrand

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Florence BRUGNON, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

University Hospital, Clermont-Ferrand

References

Pons-Rejraji H, Vorilhon S, Difrane A, Dollet S, Bourgne C, Berger M, Chaput L, Pereira B, Bouche C, Drevet JR, Brugnon F. Beneficial effects of hypotaurine supplementation in preparation and freezing media on human sperm cryo-capacitation and DNA quality. Basic Clin Androl. 2021 Nov 4;31(1):26. doi: 10.1186/s12610-021-00144-6.

Reference Type: DERIVED

PMID: 34732137 (View on PubMed)

Other Identifiers

2014 HYPOTAURINE

Identifier Type: -

Identifier Source: org_study_id