Study of Sperm Molecular Factors Implicated in Male Fertility
NCT ID: NCT00481403
Last Updated: 2022-04-01
Study Results
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Basic Information
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COMPLETED
100 participants
OBSERVATIONAL
2006-05-31
2016-12-31
Brief Summary
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Subsequently, there is a need for accurate diagnostic tools in this sense and microarray technology applied to sperm analysis emerges as a promising field
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Detailed Description
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Recent investigations about sperm mRNA contents have described the relevance of the sperm mRNA stock in fertilization and early embryo development in several species. (2) Although sperm is a quiescent cell from the translational point of view, several functional mRNAs that will be delivered into the oocyte after fertilization, that were synthesized in an earlier phase of the spermatogenesis process can be found (3, 4).
The fertile male transcriptome (stock of mRNAs within the sperm of a male able to have progeny) has been already described (5, 6), confirming the expression of thousands of sequence tags with different intensity of expression.
Moreover, several investigations have demonstrated that a differential expression of some key mRNAs is found in infertile males in comparison with fertile males (1, 7).
Nevertheless, there is no information available neither about the characteristics of the stock sperm mRNAs on infertile males or the differences between fertile and infertile men, although several authors have hypothesized that sperm microarray analysis will be the future in the male infertility diagnosis (2, 8-11).
Microarray technology provides information about a wide range of mRNAs expression within a single experiment, permitting to analyze complete sperm expression profiles (SEP) in cells or tissues. Bioinformatics can help in the organization of such amount of results by following logical processes of gene expression grouping, and analyzing statistically these findings.
Our aim with this work was to compare the SEP in spermatozoa obtained from males with idiopathic infertility versus those from sperm donors of proven fertility by employing microarray technology followed by a functional analysis, in order to determine the genes, sequences and biological processes involved in the sperm physiology that are different in infertile vs. fertile males.
Conditions
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Study Design
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COHORT
PROSPECTIVE
Interventions
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Microarray analysis
Microarray analysis
Microarray
Microarray
Eligibility Criteria
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Inclusion Criteria
Exclusion Criteria
18 Years
50 Years
MALE
Yes
Sponsors
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Instituto Valenciano de Infertilidad, IVI VALENCIA
OTHER
Responsible Party
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Nicolas Garrido
Director of Andrology IVI Valencia
Principal Investigators
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Nicolas Garrido, PhD
Role: PRINCIPAL_INVESTIGATOR
Instituto Universitario IVI
Locations
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Instituto Universitario Ivi
Valencia, , Spain
Countries
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References
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1. Garrido N, Meseguer M, Alvarez J, Simon C, Pellicer A, Remohi J. Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content in ejaculated spermatozoa from fertile and infertile men. Fertil Steril. 2004 Oct;82 Suppl 3:1059-66. 2. Krawetz SA. Paternal contribution: New insights and future challenges. Nat Rev Genet. 2005;6:633-42. 3. Kramer JA, Krawetz SA. RNA in spermatozoa: Implications for the alternative haploid genome. Mol Hum Reprod. 1997;3:473-8. 4. Wykes SM, Visscher DW, Krawetz SA. Haploid transcripts persist in mature human spermatozoa. Mol Hum Reprod. 1997; 3:15-9.
Other Identifiers
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VLC-NG-0506-(1003-C-068-JH)
Identifier Type: -
Identifier Source: org_study_id
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