Presence of SARS-CoV-2 (COVID-19) in Semen: Impact on Fertility Preservation in Male Oncological Patients ?

NCT ID: NCT04487639

Last Updated: 2024-02-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

129 participants

Study Classification

INTERVENTIONAL

Study Start Date

2020-07-10

Study Completion Date

2023-12-31

Brief Summary

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During the COVID-19 pandemic, the French Agency of Biomedicine has recommended maintaining fertility preservation for patients requiring immediate oncological treatments exhibiting gonadotoxic effects. However, no study has examined the presence of SARS-CoV-2 in sperm from cancer patients. This study aims therefore to detect the presence of SARS-CoV-2, specifically in the seminal fluid and the spermatozoa fractions of cancer patient semen. The investigators will determine if the virus presence in sperm is associated with its presence in the nasal swabs, COVID symptoms, specific serological profiles and particular oncological pathologies/treatments.

Detailed Description

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To perform this, all patient undergoing oncological fertility preservation will be evaluated for COVID-19 symptomatology (fever, cough, headache, myalgia, diarrhea, anosmia, pharyngodynia). In addition, a nasopharyngeal swab for SARS-CoV-2 research by RT- qPCR will be performed on the day of semen collection. On the same day, serological tests will be carried out, and 30 to 50 days after, according to HAS specifications with methods validated by the National Reference Center. Seminal fluid and spermatozoa will be separated by density gradient centrifugation for a posteriori molecular analysis of SARS-CoV-2 presence.

Will thus be measured, within the same ejaculate, the concordance between the presence of SARS-CoV-2 in the seminal fluid and in the sperm cells fraction.

The investigators will also determine if the virus presence in the sperm is related with :

* (i) the presence of SARS-CoV-2 in the nasal swab;
* (ii) patient symptomatology;
* (iii) a specific serological profile;
* (iv) a particular oncological pathology and / or treatment. The investigators may also find out if the presence of the SARS-CoV-2 in semen affects sperm quality.

This study will be the first one examining the presence of SARS-CoV-2 in semen from cancer patients. This will guarantee the safety of fertility preservation procedures during the COVID-19 pandemic.

Conditions

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Sperm Preservation in Oncological Patients Fertility Cryopreservation

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Cohort : patients needing oncofertility preservation

Group Type EXPERIMENTAL

SARS-CoV-2 research in nasopharyngeal swab, sperm and serologics

Intervention Type DIAGNOSTIC_TEST

a nasopharyngeal swab for SARS-CoV-2 research by RT- qPCR will be performed on the day of semen collection. On the same day, serological tests will be carried out, and 30 to 50 days after, according to HAS specifications with methods validated by the National Reference Center

Interventions

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SARS-CoV-2 research in nasopharyngeal swab, sperm and serologics

a nasopharyngeal swab for SARS-CoV-2 research by RT- qPCR will be performed on the day of semen collection. On the same day, serological tests will be carried out, and 30 to 50 days after, according to HAS specifications with methods validated by the National Reference Center

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

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Inclusion Criteria

* Man above 18 years of age, of reproductive age, needing oncofertility preservation through spermatozoa cryopreservation prior to a putative gonadotoxic treatment during the COVID-19 pandemic.
* Symptomatic and asymptomatic COVID-19 patients. A patient is considered as symptomatic if he presents one or several of the following clinical signs: fever, cough, cephalalgia, myalgia, diarrhea, anosmia, pharyngodynia.
* A sufficient number of remaining spermatozoa straws to perform at least six ART attempts

Exclusion Criteria

* Azoospermia
* Severe Oligo-Astheno-Teratozoospermia (OAT) or Cryptozoospermia
* Semen collection failure
* Non-emergency fertility preservation or treatment with low gonadotoxic risk
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Agence de La Biomédecine

OTHER_GOV

Sponsor Role collaborator

University Hospital, Clermont-Ferrand

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Florence BRUGNON, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

CHU de Clermont-Ferrand

Locations

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AP-Hôpitaux de Paris - Hôpital Jean Verdier - Service de Biologie de la reproduction

Bondy, , France

Site Status

AP-Hôpitaux de Paris - Hôpital Antoine Béclére - Service de Biologie de la reproduction et AMP DPI CECOS

Clamart, , France

Site Status

CHU de Clermont-Ferrand - Laboratoire de Virologie

Clermont-Ferrand, , France

Site Status

CHU de Clermont-Ferrand

Clermont-Ferrand, , France

Site Status

CHU de Dijon - Laboratoire de Biologie de la Reproduction-CECOS

Dijon, , France

Site Status

CHU de Grenoble - Laboratoire d'Aide à la Procréation CECOS

Grenoble, , France

Site Status

Hospices Civils de Lyon - Service AMP-CECOS

Lyon, , France

Site Status

Hôpital de la Conception - AP-Hôpitaux de Marseille - Service AMP-CECOS

Marseille, , France

Site Status

CHU de Nancy - Laboratoire de biologie de la reproduction - CECOS

Nancy, , France

Site Status

AP-Hôpitaux de Paris - Hôpital Tenon - Service de Biologie de la reproduction - CECOS

Paris, , France

Site Status

AP-Hôpitaux de Paris - Hôpital Cochin - Service d'Histologie-Embryologie-Biologie de la reproduction

Paris, , France

Site Status

Centre Hospitalier intercommunal (CHI) de Poissy Saint Germain en Laye

Poissy, , France

Site Status

CHU de Reims - Service de Biologie de la Reproduction - CECOS Champagne-Ardenne de Reims

Reims, , France

Site Status

CHU de Rouen Normandie - Service Biologie de la Reproduction-CECOS

Rouen, , France

Site Status

CHU de Strasbourg - Service de Biologie de la reproduction

Strasbourg, , France

Site Status

CHU de Toulouse - CECOS Midi-Pyrénées

Toulouse, , France

Site Status

Countries

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France

Other Identifiers

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2020-A01409-30

Identifier Type: OTHER

Identifier Source: secondary_id

RNI 2020 BRUGNON

Identifier Type: -

Identifier Source: org_study_id

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