Serum Amyloid A Protein And Fetuin A Levels

NCT ID: NCT02433405

Last Updated: 2015-05-05

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 60 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2013-12-31
• Study Completion Date: 2014-11-30

Brief Summary

The purpose of this study was to evaluate acute phase proteins (APPs) Fetuin-A and Serum Amyloid A (SAA) levels in gingival crevicular fluid (GCF) and serum samples in periodontal health and disease.

Detailed Description

The study population consisted totally 60 subjects consulted to Bulent Ecevit University, Faculty of Dentistry, Department of Periodontology whose age and sex were matched.

According to study design, subjects were divided into three groups based on their periodontal parameters. Patients suffering from chronic periodontitis formed the group 1 (n: 20), patients with plaque induced gingivitis formed the group 2 (n:20) and periodontal healthy patients were inserted in group 3 (n:20).

After gently drying the area, GCF was sampled with filter paper using the intracrevicular method (Periopaper, ProFlow, Inc., Amityville, New York, USA).Two milliliters (ml) of peripheral blood was collected from each individual with sterile syringe from the antecubital fossa by venipuncture, and to separate serum component sample centrifuged at 3000g for 5 min, and immediately stored at -40°C until assayed.

The quantity of Fetuin-A and SAA protein concentration in the samples were analyzed by sandwich enzyme-linked immunosorbent assay (ELISA) procedures using commercially available kits.

The Spearman's rank correlation test was used to detect the relationship between GCF and serum SAA and Fetuin-A with GI and CAL. All tests were performed using statistical software (SPSS Inc., version 22.0, Chicago, IL, USA). The mean ± standard deviation with mean rank values were calculated for each parameter, based on the patients as the statistical unit. P < 0.05 was considered to be statistically significant.

Conditions

Periodontal Diseases

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: CROSS_SECTIONAL

Study Groups

Group 1

Chronic Periodontitis-serum amyloid A, Fetuin-A

Fetuin-A

Intervention Type: BIOLOGICAL

Acute phase protein

Serum Amyloid A

Intervention Type: BIOLOGICAL

Acute phase protein

Group 2

Plaque induced Gingivitis-serum amyloid A, Fetuin-A

Fetuin-A

Intervention Type: BIOLOGICAL

Acute phase protein

Serum Amyloid A

Intervention Type: BIOLOGICAL

Acute phase protein

Group 3

Control Group-serum amyloid A, Fetuin-A

Fetuin-A

Intervention Type: BIOLOGICAL

Acute phase protein

Serum Amyloid A

Intervention Type: BIOLOGICAL

Acute phase protein

Interventions

Fetuin-A

Acute phase protein

Intervention Type: BIOLOGICAL

Serum Amyloid A

Acute phase protein

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

• GI>1, PPD≥5mm, CAL≥5mm with alveolar bone loss radiographically.
• Systematically healthy patients

• No alveolar bone and attachment loss
• Inflammation signs such as redness, edema and increased BOP levels,
• GI≥2, PPD≤3mm, CAL≤3mm.
• Systematically healthy patients

• No bone and attachment loss,
• GI=0, PPD≤3mm, CAL≤3mm
• Systematically healthy patients

Exclusion Criteria

• Aggressive Periodontitis,
• Oral pathologies,
• Patients with any other systemic diseases,
• Pregnant women and those in the lactation period,
• Patients with smoking habit and taking medication
• Patients received periodontal therapy in last 6 months

• Minimum Eligible Age: 28 Years
• Maximum Eligible Age: 45 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Bulent Ecevit University

OTHER

Sponsor Role: lead

Responsible Party

ÇİĞDEM COŞKUN TÜRER

Dr.

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

ÇİĞDEM COŞKUN TÜRER, PhD

Role: PRINCIPAL_INVESTIGATOR

BULENT ECEVIT UNIVERSITY FACULTY OF DENTISTRY DEPARTMENT OF PERIODONTOLOGY

Other Identifiers

SAAFA1

Identifier Type: -

Identifier Source: org_study_id