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Evaluation of Free Aminoacids in Saliva as A Diagnostic Biomarker of Periodontal Disease

NCT ID: NCT04642716

Last Updated: 2020-11-24

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

60 participants

Study Classification

INTERVENTIONAL

Study Start Date

2015-04-20

Study Completion Date

2017-08-03

Brief Summary

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Amino acids (AAs) are organic molecules that are associated with inflammation and innate immunity diseases with critical roles in the cell repair and protection process. Our aim was to identify the free amino acids in saliva of a group of periodontitis patients and healthy individuals and additionally to assess their levels in different periodontal disease types and compare it to the healthy individuals.

Detailed Description

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The study consisted of three groups: healthy individuals (control(C); n=20), Stage III Grade B generalized periodontitis (GP-B; n=20), and Stage III Grade C generalized periodontitis (GP-C; n=20). Clinical periodontal parameters (plaque index (PI), gingival index (GI), probing depth (PPD), gingival recession (GR), clinical attachment level (CAL) and bleeding on probing (BOP)) were measured. The amino acid analysis in saliva was accomplished by liquid chromatography-mass spectrometry (LC MS/MS) as mean concentration.

Conditions

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Periodontitis Diagnoses Disease

Keywords

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Saliva Amino acids Periodontitis

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Saliva of periodontitis patients collected for analyzing free amino acids.
Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

NONE

Study Groups

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Salivary free amino acids profile observation

Saliva samples of periodontitis patients and healthy controls were collected. The AA analysis of the saliva was performed by LC-MS/MS by using the Thermo Scientific TSQ Quantum Access MAX (Thermo Scientific, Schaumburg, IL, USA) .

Group Type OTHER

Saliva collection of patients and salivary free amino acids analysis

Intervention Type OTHER

Interventions

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Saliva collection of patients and salivary free amino acids analysis

Intervention Type OTHER

Other Intervention Names

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Salivary free amino acids in patients with periodontitis

Eligibility Criteria

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Inclusion Criteria

systemically healthy, clinical diagnosis of periodontitis, clinical diagnosis of periodontal health

Exclusion Criteria

history of regular use of systemic antibiotics, anti-inflammatory, or antioxidant drugs (previous 3 months); nonsurgical periodontal treatment (previous 6 months); surgical periodontal treatment (previous 12 months); presence of \<10 teeth; current medications affecting gingival health (calcium channel blockers, phenytoin, cyclosporine, and hormone replacement therapy); diabetes diagnosis; rheumatoid arthritis diagnosis; and pregnancy, lactating, smoking, or excessive alcohol consumption.
Minimum Eligible Age

18 Years

Maximum Eligible Age

65 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Istanbul Medipol University Hospital

OTHER

Sponsor Role lead

Responsible Party

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Nur Balci, DDs, PhD

Dr. Nur Balci

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Istanbul Medipol University, School of Dentistry

Istanbul, , Turkey (Türkiye)

Site Status

Countries

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Turkey (Türkiye)

References

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Erratum for Wang et al. Glycine stimulates protein synthesis and inhibits oxidative stress in pig small intestinal epithelial cells. J Nutr 2014;144:1540-8. J Nutr. 2016 Sep;146(9):1813. doi: 10.3945/jn.116.236612. No abstract available.

Reference Type BACKGROUND
PMID: 27587752 (View on PubMed)

Li P, Yin YL, Li D, Kim SW, Wu G. Amino acids and immune function. Br J Nutr. 2007 Aug;98(2):237-52. doi: 10.1017/S000711450769936X. Epub 2007 Apr 3.

Reference Type BACKGROUND
PMID: 17403271 (View on PubMed)

Cruzat VF, Krause M, Newsholme P. Amino acid supplementation and impact on immune function in the context of exercise. J Int Soc Sports Nutr. 2014 Dec 14;11(1):61. doi: 10.1186/s12970-014-0061-8. eCollection 2014.

Reference Type BACKGROUND
PMID: 25530736 (View on PubMed)

Song Zh, Tong G, Xiao K, Jiao le F, Ke Yl, Hu Ch. L-cysteine protects intestinal integrity, attenuates intestinal inflammation and oxidant stress, and modulates NF-kappaB and Nrf2 pathways in weaned piglets after LPS challenge. Innate Immun. 2016 Apr;22(3):152-61. doi: 10.1177/1753425916632303. Epub 2016 Feb 25.

Reference Type BACKGROUND
PMID: 26921254 (View on PubMed)

Sido B, Seel C, Hochlehnert A, Breitkreutz R, Droge W. Low intestinal glutamine level and low glutaminase activity in Crohn's disease: a rational for glutamine supplementation? Dig Dis Sci. 2006 Dec;51(12):2170-9. doi: 10.1007/s10620-006-9473-x. Epub 2006 Nov 1.

Reference Type BACKGROUND
PMID: 17078002 (View on PubMed)

Onder C, Kurgan S, Altingoz SM, Bagis N, Uyanik M, Serdar MA, Kantarci A, Gunhan M. Impact of non-surgical periodontal therapy on saliva and serum levels of markers of oxidative stress. Clin Oral Investig. 2017 Jul;21(6):1961-1969. doi: 10.1007/s00784-016-1984-z. Epub 2016 Nov 2.

Reference Type BACKGROUND
PMID: 27807715 (View on PubMed)

Dede FO, Ozden FO, Avci B. 8-hydroxy-deoxyguanosine levels in gingival crevicular fluid and saliva in patients with chronic periodontitis after initial periodontal treatment. J Periodontol. 2013 Jun;84(6):821-8. doi: 10.1902/jop.2012.120195. Epub 2012 Aug 16.

Reference Type BACKGROUND
PMID: 22897655 (View on PubMed)

Rajda C, Tajti J, Komoroczy R, Seres E, Klivenyi P, Vecsei L. Amino acids in the saliva of patients with migraine. Headache. 1999 Oct;39(9):644-9. doi: 10.1046/j.1526-4610.1999.3909644.x.

Reference Type BACKGROUND
PMID: 11279959 (View on PubMed)

Le A, Ng A, Kwan T, Cusmano-Ozog K, Cowan TM. A rapid, sensitive method for quantitative analysis of underivatized amino acids by liquid chromatography-tandem mass spectrometry (LC-MS/MS). J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 1;944:166-74. doi: 10.1016/j.jchromb.2013.11.017. Epub 2013 Nov 18.

Reference Type BACKGROUND
PMID: 24316529 (View on PubMed)

Balci N, Kurgan S, Cekici A, Cakir T, Serdar MA. Free amino acid composition of saliva in patients with healthy periodontium and periodontitis. Clin Oral Investig. 2021 Jun;25(6):4175-4183. doi: 10.1007/s00784-021-03977-7. Epub 2021 May 11.

Reference Type DERIVED
PMID: 33977387 (View on PubMed)

Other Identifiers

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10840098-327

Identifier Type: -

Identifier Source: org_study_id