Fatty Acid Metabolism and Insulin Sensitivity After Exercise in Obesity

NCT ID: NCT01451957

Last Updated: 2016-11-23

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 7 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2003-08-31
• Study Completion Date: 2011-04-30

Brief Summary

Alterations in fatty acid mobilization and oxidation may be primary adaptations responsible for the improvements in metabolic health after a single session of endurance exercise. The investigators will determine the effect of a single session of endurance exercise on whole-body fatty acid mobilization and oxidation, IMTG concentration and the expression of factors that regulate these processes in skeletal muscle of 11 women with abdominal obesity (age: 18-45y). In addition, the investigators will evaluate how these factors, and exercise, effect insulin signalling and insulin sensitivity. Every effort will be made to recruit subjects from ethnic and minority groups. Before participating in the study, subjects will be informed of all the procedures and potential risks, and they will sign an informed consent form approved by The University of Michigan Institutional Review Board. Eligible volunteers will participate in three separate trials, in a randomized order. In two trials subjects will eat exactly the same amount of calories, except in one trial they will exercise (eucaloric + exercise) and in the other trial they will remain sedentary (hypercaloric). In a third trial subjects will again remain sedentary but instead they will ingest appropriate calories to maintain caloric balance (eucaloric + sedentary). By doing this the investigators are also able to investigate the effect of acute caloric perturbations on insulin sensitivity, because it is possible that the enhanced insulin sensitivity evident after exercise, as compared to the sedentary state, is due to caloric deficit and not the exercise bout, per se.

Conditions

Obesity

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Exercise

90 min exercise on Day 1

Group Type: EXPERIMENTAL

Exercise - caloric balance

Intervention Type: OTHER

90 min exercise on Day 1 with meals afterward resulting in caloric balance for the day (i.e.; extra kcals provided to account for energy expended during exercise)\]

Sedentary Control

Subjects remain sedentary on Day 1 and will either consume a hyper-caloric or a caloric balanced diet

Group Type: EXPERIMENTAL

No Exercise - hyper-caloric diet

Intervention Type: OTHER

Subjects remain sedentary on Day 1 and they ingest meals that are identical to those ingested on the day they performed exercise (i.e.; more calories ingested than expended because no exercise performed).

No exercise - caloric balance

Intervention Type: OTHER

Subjects remain sedentary on Day 1 and they ingest a similar proportion of macronutrients however, the total calories ingested will be lower than the other two trials, resulting in caloric balance for the day.

Interventions

Exercise - caloric balance

90 min exercise on Day 1 with meals afterward resulting in caloric balance for the day (i.e.; extra kcals provided to account for energy expended during exercise)\]

Intervention Type: OTHER

No Exercise - hyper-caloric diet

Subjects remain sedentary on Day 1 and they ingest meals that are identical to those ingested on the day they performed exercise (i.e.; more calories ingested than expended because no exercise performed).

Intervention Type: OTHER

No exercise - caloric balance

Subjects remain sedentary on Day 1 and they ingest a similar proportion of macronutrients however, the total calories ingested will be lower than the other two trials, resulting in caloric balance for the day.

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Premenopausal; Body mass index 30-40 kg/m2; Waist circumference >88 cm

Exclusion Criteria

• Evidence of metabolic or cardiovascular disease; Pregnancy; Hyperlipidemia (fasting plasma triglyceride concentration > 150 mg/dl); Hematocrit < 34%; Undertaking regular exercise (i.e., >2 times/week)

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 45 Years
• Eligible Sex: FEMALE
• Accepts Healthy Volunteers: Yes

Sponsors

University of Michigan

OTHER

Sponsor Role: lead

Responsible Party

Jeffrey F Horowitz

Associate Professor, Movement Science, School of Kinesiology

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Jeffrey F Horowitz, Ph.D.

Role: PRINCIPAL_INVESTIGATOR

University of Michigan

Locations

Michigan Clincal Research Unit

Ann Arbor, Michigan, United States

Countries

United States

Other Identifiers

03-0165

Identifier Type: -

Identifier Source: org_study_id