Do Changes in Plasma Osmolality Influence Ventilation?

NCT ID: NCT01008644

Last Updated: 2012-10-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

EARLY_PHASE1

Total Enrollment

26 participants

Study Classification

INTERVENTIONAL

Study Start Date

2009-11-30

Study Completion Date

2010-11-30

Brief Summary

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Primary hypothesis: osmolality changes influence the sensitivity of the respiratory center to carbon dioxide, hyponatraemia causing hyperventilation, and hypernatraemia depressing ventilation.

Secondary hypothesis: There are gender differences in the sensitivity to osmolality changes.

10 women and 10 men will on different occasions drink water or receive hypertonic saline intravenously, in order to lower or increase plasma osmolality. The women will participate during both faces of the menstruation cycle. On each occasion the subject´s sensitivity to carbon dioxide will be tested, and blood samples will be drawn for analysis of blood gases,electrolyte and osmolality.Subjects who interrupt participation before completion of all planned occasions, will be substituted, so that 10 subjects of either sex will have participated as planned. All results from all participants will be analyzed.

Detailed Description

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Healthy volunteers will on different occasions be subject to reduced plasma osmolality caused by drinking water, and increased osmolality caused by intravenous infusion of hypertonic saline.Before and after each osmolality change, sensitivity to carbon dioxide will be tested by partial rebreathing through a so called Bain-system. Throughout the whole experiment heart rate, blood pressure and oxygen saturation will be recorded.Blood samples will be collected before each rebreathing test and every 20 minutes during the two hours of water or salt load. Urine will be collected and analysed.

Conditions

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Hyponatraemia Hypernatraemia

Keywords

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carbon dioxide ventilation gender osmolality menstruation cycle

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

FACTORIAL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Saline

The subjects will receive saline 3% intravenously for 2 hours, the volume calculated as 0.1 ml/kg/min.

Group Type EXPERIMENTAL

Water

Intervention Type OTHER

The subjects will drink tap water for 2 hours, volume calculated according to weight: 20ml/kg/hour.

Saline 3%

Intervention Type OTHER

The subjects will receive saline 3% intravenously for 2 hours, the volume calculated as 0.1 ml/kg/min

Water

The subjects will drink tap water for 2 hours, the volume calculated as 20ml/kg/hour

Group Type EXPERIMENTAL

Water

Intervention Type OTHER

The subjects will drink tap water for 2 hours, volume calculated according to weight: 20ml/kg/hour.

Saline 3%

Intervention Type OTHER

The subjects will receive saline 3% intravenously for 2 hours, the volume calculated as 0.1 ml/kg/min

Interventions

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Water

The subjects will drink tap water for 2 hours, volume calculated according to weight: 20ml/kg/hour.

Intervention Type OTHER

Saline 3%

The subjects will receive saline 3% intravenously for 2 hours, the volume calculated as 0.1 ml/kg/min

Intervention Type OTHER

Other Intervention Names

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saline 3%

Eligibility Criteria

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Inclusion Criteria

* Females with regular menstruations, males

Exclusion Criteria

* Consumption of nicotine, BMI \> 26,
* pregnancy,any hormone treatment,
* treatment with diuretics,
* diabetes or kidney disease,
* BMI \> 26,
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Vibeke Moen

OTHER

Sponsor Role lead

Responsible Party

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Vibeke Moen

MD, Department of Anaesthesia

Responsibility Role SPONSOR_INVESTIGATOR

Principal Investigators

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Lars Irestedt. MD PhD

Role: STUDY_DIRECTOR

Department of Anaesthesia and Intensive Care , Karolinska University Hospital, Stockholm

Locations

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Department of Anaesthesia and Intensive care, Kalmar County Hospital

Kalmar, Kalmar County, Sweden

Site Status

Countries

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Sweden

References

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Jennings DB. The physicochemistry of [H+] and respiratory control: roles of PCO2, strong ions, and their hormonal regulators. Can J Physiol Pharmacol. 1994 Dec;72(12):1499-512. doi: 10.1139/y94-216.

Reference Type BACKGROUND
PMID: 7736341 (View on PubMed)

Weissgerber TL, Wolfe LA, Hopkins WG, Davies GA. Serial respiratory adaptations and an alternate hypothesis of respiratory control in human pregnancy. Respir Physiol Neurobiol. 2006 Aug;153(1):39-53. doi: 10.1016/j.resp.2005.09.004. Epub 2005 Nov 28.

Reference Type BACKGROUND
PMID: 16311079 (View on PubMed)

Heenan AP, Wolfe LA. Plasma osmolality and the strong ion difference predict respiratory adaptations in pregnant and nonpregnant women. Can J Physiol Pharmacol. 2003 Sep;81(9):839-47. doi: 10.1139/y03-072.

Reference Type BACKGROUND
PMID: 14614519 (View on PubMed)

Other Identifiers

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M-126-09

Identifier Type: -

Identifier Source: org_study_id