Exercise-induced Changes in Exosomes

NCT ID: NCT05616234

Last Updated: 2023-06-15

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 12 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2022-11-07
• Study Completion Date: 2023-02-17

Brief Summary

The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.

Conditions

Exosomes; Connective Tissue; Exercise

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Exercise trial

Participants will perform a bout of exercise. In addition, a baseline and post-exercise blood sample will be drawn.

Group Type: EXPERIMENTAL

Resistance Exercise

Intervention Type: PROCEDURE

Participants will perform a bout of lower body resistance exercise consisting of leg press, leg extension and leg curls.

Interventions

Resistance Exercise

Participants will perform a bout of lower body resistance exercise consisting of leg press, leg extension and leg curls.

Intervention Type: PROCEDURE

Eligibility Criteria

Inclusion Criteria

• Young healthy adults (18-35 y)
• Experience with exercise (>1y)

Exclusion Criteria

• Pregnancy
• Smoking
• Receiving any medication that may interfere with the study outcomes

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 35 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

University of California, Davis

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Keith Baar, PhD

Role: PRINCIPAL_INVESTIGATOR

University of California, Davis

Locations

Hickey Laboratory

Davis, California, United States

Countries

United States

Other Identifiers

1956959

Identifier Type: -

Identifier Source: org_study_id