Protein Digestibility of Whey and Zein.

NCT ID: NCT04207372

Last Updated: 2019-12-20

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

10 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-05-04

Study Completion Date

2019-01-17

Brief Summary

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The evaluation of protein quality has top priority according to Food and Agricultural Organization of the United Nations. However, one aspect of protein quality, namely the digestibility of protein is largely unknown. A database on this matter is lacking as it is difficult to measure ileal digestibility in humans.

Detailed Description

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Objective: This study aims to determine the digestibility of amino acids in two protein sources in men and women with normally functioning ileostomies.

Study design: Intervention study with 6 test days, testing 3 meals each twice. Study population: Eight men and women with normally functioning ileostomies will be recruited for the study.

Intervention: On six separate test days, subjects will receive a meal, consisting of a drink and biscuit. With this, two different protein containing meals, i.e. zein and whey, and a protein-free meal is tested; these three treatments are given at two occasions, thus in total 6 meals are tested. After consumption of the meal, subjects collect their digesta for nine hours.

Main study parameters/endpoints: Ileal amino acid digestibility of zein and whey protein isolate.

Nature and extent of the burden and risks associated with participation, benefit and group relatedness: The subject will not benefit from the study except for the contribution to scientific research. The risk of participating to this study is low, some gastrointestinal discomfort could occur. Subjects have to come to the research facility seven times, a screening visit of 1 hour, and 6 experimental days of 10 hours. Subjects will be financially compensated for participation.

Conditions

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Biological Availability

Keywords

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Digestibility Whey Zein Protein Ileostomates

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Intervention study with 6 test days, testing 3 meals each twice
Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

SINGLE

Participants
Allocation of different proteins(or no protein) is blinded from participant.

Study Groups

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Whey protein isolate

Group Type EXPERIMENTAL

whey protein isolate

Intervention Type DIETARY_SUPPLEMENT

whey protein isolate

Zein

Group Type EXPERIMENTAL

Zein

Intervention Type DIETARY_SUPPLEMENT

zein protein

Protein-free

Group Type PLACEBO_COMPARATOR

protein-free

Intervention Type OTHER

protein free

Interventions

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whey protein isolate

whey protein isolate

Intervention Type DIETARY_SUPPLEMENT

Zein

zein protein

Intervention Type DIETARY_SUPPLEMENT

protein-free

protein free

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Male or female
* Age between 18 and 60
* Willing to eat animal protein
* Have a well-established and normally functioning terminal ileostomy, with stomal faeces of normal physiological consistency
* Good general health, meaning subjects should be well-recovered from the ileostomy operation, their underlying disease should be cured or in remission and they should not have any other major health problems

Exclusion Criteria

* Taken antibiotics or medication that majorly impair small intestinal digestion and absorption within eight weeks of participating in the study.
* Have been pregnant or breastfeeding in the last 12 months, or plan to become pregnant during the study
* Having renal impairment, coeliac disease or diabetes
* Drug abuse
* Alcohol consumption of \>14 units per week.
* Having an allergy or intolerance to dairy, corn products or fructose
* Currently taking protein supplements and would not be willing to stop using these during the study
* Being on a controlled diet or dietary weight loss regimen during the two weeks prior to the start of this study and/or during the study
* Personnel of division of Human Nutrition, Wageningen University..
* Current participation in other research from the Division of Human Nutrition
* Not having a general practitioner
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Massey University

OTHER

Sponsor Role collaborator

Global Dairy Platform Incorporated

UNKNOWN

Sponsor Role collaborator

Wageningen University

OTHER

Sponsor Role lead

Responsible Party

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Geleijnse

Prof. Dr.

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Division of Human Nutrition, Wageningen University

Wageningen, Gelderland, Netherlands

Site Status

Countries

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Netherlands

References

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van der Wielen N, de Vries S, Gerrits WJ, Lammers-Jannink K, Moughan PJ, Mensink M, Hendriks W. Presence of Unabsorbed Free Amino Acids at the End of the Small Intestine Indicates the Potential for an Increase in Amino Acid Uptake in Humans and Pigs. J Nutr. 2023 Mar;153(3):673-682. doi: 10.1016/j.tjnut.2023.01.038. Epub 2023 Feb 9.

Reference Type DERIVED
PMID: 36809852 (View on PubMed)

Other Identifiers

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NL63446.081.17

Identifier Type: -

Identifier Source: org_study_id