Dairy and Plant Amino Acid Uptake. An Explorative Study

NCT ID: NCT05813769

Last Updated: 2023-06-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

12 participants

Study Classification

INTERVENTIONAL

Study Start Date

2023-05-09

Study Completion Date

2023-06-21

Brief Summary

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To optimize the environmental sustainability, a protein transition to more plant-based protein sources is required. However, the protein quality of plant-based sources is lower than that of the dairy proteins casein and whey, which contain high levels of essential amino acids. The amino acid absorption characteristics of many plant-based proteins are unknown.

The present study aims to estimate differences in postprandial plasma total essential amino acids (TEAA) profiles after protein consumption of different protein sources in blood of healthy participants. This explorative study has a randomized, cross-over, double-blind, controlled design.

Detailed Description

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Conditions

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Amino Acid Plasma Profile

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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Dairy protein 1

Group Type ACTIVE_COMPARATOR

Dairy protein

Intervention Type DIETARY_SUPPLEMENT

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Dairy protein 2

Group Type ACTIVE_COMPARATOR

Dairy protein

Intervention Type DIETARY_SUPPLEMENT

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Plant protein 1

Group Type EXPERIMENTAL

Plant protein

Intervention Type DIETARY_SUPPLEMENT

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Plant protein 2

Group Type EXPERIMENTAL

Plant protein

Intervention Type DIETARY_SUPPLEMENT

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Plant protein 2 + added AA

Group Type EXPERIMENTAL

Plant protein

Intervention Type DIETARY_SUPPLEMENT

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Plant protein 2 + Dairy protein 2

Group Type EXPERIMENTAL

Dairy protein

Intervention Type DIETARY_SUPPLEMENT

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Plant protein

Intervention Type DIETARY_SUPPLEMENT

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Interventions

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Dairy protein

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Intervention Type DIETARY_SUPPLEMENT

Plant protein

During each visit, research subjects will receive the product dissolved in water, representing a 20g protein load.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Apparently healthy men and women;
* Age ≥18 and ≤40 years;
* Body mass index (BMI) ≥18.5 and ≤30 kg/m2 ;
* Having veins suitable for blood sampling via a catheter (judged by study nurse/ medical doctor).

Exclusion Criteria

* Any self-reported metabolic, gastrointestinal, inflammatory or chronic disease (such as anemia, diabetes, hepatitis, cardiovascular disease);
* Having a history of medical or surgical events that may significantly affect the study outcome;
* Anaemia (Haemoglobin (Hb) values \<7.5 mmol/L for women and \<8.5 mmol/L for men), as assessed by finger prick blood during screening visit;
* Having a food or protein allergy
* Use of glucose lowering drugs, insulin;
* Use of medication that may impact gastric emptying (e.g. gastric acid inhibitors or laxatives);
* Use of antibiotic treatment less than 1 month before start of the study and during the study;
* Use of anti-depressives as a treatment for depression;
* Use of protein supplements (must be stopped 1 week before the first test day);
* Reported weight loss or weight gain of \> 3 kg in the month prior to pre-study screening, or intention to lose weight during the study period;
* Reporting to follow or having planned a slimming or medically prescribed diet.
* Not willing to keep a stable lifestyle during the study period;
* Recent blood donation (\<1 month prior to test day 1 of the study) or not willing to stop donation during and 1 month after the study;
Minimum Eligible Age

18 Years

Maximum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Wageningen University and Research

OTHER

Sponsor Role collaborator

FrieslandCampina

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Wageningen Food & Biobased Research, Food, Health & Consumer Research

Wageningen, Gelderland, Netherlands

Site Status

Countries

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Netherlands

Other Identifiers

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DaPa

Identifier Type: -

Identifier Source: org_study_id

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