Influence of Protein Hydrolysis on Dietary Protein Digestibility and Metabolism in Healthy Subjects

NCT ID: NCT00873951

Last Updated: 2025-10-06

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

26 participants

Study Classification

INTERVENTIONAL

Study Start Date

2006-01-31

Study Completion Date

2008-01-31

Brief Summary

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Hydrolysis of dietary protein may impact their gastrointestinal kinetics and further metabolism. The primary goal of this work was to measure the metabolic fate of intact or hydrolyzed protein using a protein of good nutritional quality, i.e., casein. A second aim of this study was to assess the true ileal digestibility of dietary protein using different methods including the hydrolyzed casein method and to measure its possible effect on endogenous intestinal N losses in humans.

Detailed Description

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Conditions

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Healthy

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

OTHER

Study Groups

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Intact casein

Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled intact casein

Group Type EXPERIMENTAL

Intestinal and metabolic exploration

Intervention Type OTHER

Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.

15N-labelled intact casein

Intervention Type DIETARY_SUPPLEMENT

standard mixed meal containing 15% of energy as 15N-labelled intact casein

Hydrolyzed casein

Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein

Group Type EXPERIMENTAL

Intestinal and metabolic exploration

Intervention Type OTHER

Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.

15N-labelled hydrolyzed casein

Intervention Type DIETARY_SUPPLEMENT

standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein

AA

Subjects undergo an intestinal and metabolic exploration following the ingestion of a standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine

Group Type EXPERIMENTAL

Intestinal and metabolic exploration

Intervention Type OTHER

Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.

mixture of AA

Intervention Type DIETARY_SUPPLEMENT

standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine

Interventions

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Intestinal and metabolic exploration

Subjects are studied for 8h following the ingestion of a standard meal containing 15% of energy as protein. Intestinal, blood and urine are collected at regular intervals.

Intervention Type OTHER

15N-labelled intact casein

standard mixed meal containing 15% of energy as 15N-labelled intact casein

Intervention Type DIETARY_SUPPLEMENT

15N-labelled hydrolyzed casein

standard mixed meal containing 15% of energy as 15N-labelled hydrolyzed casein

Intervention Type DIETARY_SUPPLEMENT

mixture of AA

standard mixed meal containing 15% of energy as a mixture of AA mimicking the composition of casein but devoid in serine

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* healthy subjects
* BMI between 18 and 25

Exclusion Criteria

* allergy to dairy proteins
* positive serology to AgHbS, HCV or HIV
* pregnancy
* abusive drug or alcohol consumption.
Minimum Eligible Age

18 Years

Maximum Eligible Age

40 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Riddet Institute

UNKNOWN

Sponsor Role collaborator

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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Centre de recherche sur volontaires

Bobigny, , France

Site Status

Countries

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France

References

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Deglaire A, Moughan PJ, Airinei G, Benamouzig R, Tome D. Intact and hydrolyzed casein lead to similar ileal endogenous protein and amino acid flows in adult humans. Am J Clin Nutr. 2020 Jan 1;111(1):90-97. doi: 10.1093/ajcn/nqz268.

Reference Type DERIVED
PMID: 31711108 (View on PubMed)

Deglaire A, Fromentin C, Fouillet H, Airinei G, Gaudichon C, Boutry C, Benamouzig R, Moughan PJ, Tome D, Bos C. Hydrolyzed dietary casein as compared with the intact protein reduces postprandial peripheral, but not whole-body, uptake of nitrogen in humans. Am J Clin Nutr. 2009 Oct;90(4):1011-22. doi: 10.3945/ajcn.2009.27548. Epub 2009 Aug 19.

Reference Type DERIVED
PMID: 19692493 (View on PubMed)

Other Identifiers

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DEGLAIRE CT

Identifier Type: -

Identifier Source: org_study_id

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