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Acute Whey Protein And Casein Supplementation: Effect On Protein Metabolism After Resistance Exercise

NCT ID: NCT04648384

Last Updated: 2020-12-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

10 participants

Study Classification

INTERVENTIONAL

Study Start Date

2016-03-20

Study Completion Date

2019-10-20

Brief Summary

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The proteins of human breast milk are the most important endocrine signaling system that promotes neonatal growth by increasing the release of insulin, insulin-like growth factor-1 (IGF-1), and the leucine-mediated mammalian target of rapamycin complex 1- (mTORC1) signaling of pancreatic β-cells. Remarkably, the branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are involved in the growth-promoting effects of milk, protein biosynthesis, and metabolism because they physiologically stimulate insulin secretion The present study sought to investigate the effects of the combination of whey protein and casein in the ratio of 80:20 ("whey protein: casein" or "casein: whey protein") as breast milk proportion on the peak and the period of the permanence of branched-chain amino acids in the blood circulation, final metabolites of protein metabolism and delayed onset muscle soreness (DOMS) after a single bout of fasting or a resistance exercise session.

Detailed Description

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The present study investigated the effects of the association of WP and casein in the ratio of 80:20, a similar ratio of human breast milk, on blood branched-chain amino acids (BCAAs) profile by HPLC technique, markers of protein metabolism (creatinine, urea, and urine nitrogen) and delayed onset muscle soreness (DOMS) after a single bout of resistance exercise. A double-blind, crossover and acute study was conducted with ten men (age 29 ± 8 y; BMI: 25.4 ± 2.9 Kg/m2; 77 ± 12 kg; 1.74 ± 0.09 m) that were randomly assigned to five supplementation treatments: WP - whey protein; CAS - casein; WP/CAS - 80% WP/20% CAS; CAS/WP - 80% CAS/ 20% WP; PLA - placebo. Participants were submitted to the following evaluations: performance sessions, blood collection for each session for the BCAAs profile determination, two food records, 3-days assessment of DOMS (24h, 48h, 72h) after each treatment.

Conditions

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Sports Nutrition Protein Supplementation

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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Protein Supplementation and Resistance exercise

Protein supplementation composed of 20 gram of whey protein or casein in different proportions (80:20) and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition

Group Type EXPERIMENTAL

Blend Protein Supplementation

Intervention Type DIETARY_SUPPLEMENT

Protein supplementation using 20 grams of whey protein or casein mix. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition

Resistance Exercise

Intervention Type OTHER

10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise

Placebo Supplementation

Placebo supplementation composed of 20 gram of Maltodextrin and fasting state 10 sets of 10 reps at 85% of 1 Maximum repetition

Group Type PLACEBO_COMPARATOR

Placebo Supplementation

Intervention Type DIETARY_SUPPLEMENT

Placebo supplementation using 20 grams of Maltodextrin. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition

Resistance Exercise

Intervention Type OTHER

10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise

Resistance Training

Resistance Exercise will be performed before supplementation and is composed by 10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise.

Group Type EXPERIMENTAL

Resistance Exercise

Intervention Type OTHER

10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise

Interventions

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Blend Protein Supplementation

Protein supplementation using 20 grams of whey protein or casein mix. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition

Intervention Type DIETARY_SUPPLEMENT

Placebo Supplementation

Placebo supplementation using 20 grams of Maltodextrin. Acute Resistance training, 10 sets of 10 reps at 85% of 1Maximum repetition

Intervention Type DIETARY_SUPPLEMENT

Resistance Exercise

10 sets of 10 repetitions at 85% of 1 Maximum repetition in leg press exercise

Intervention Type OTHER

Other Intervention Names

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Resistance Training Resistance Training

Eligibility Criteria

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Inclusion Criteria

* Healthy
* practice resistance training at least 3x a week

Exclusion Criteria

* Smokers
* Muscle injures
* cardiovascular diseases
* Diabetes mellitus
* Lactose intolerance
Minimum Eligible Age

20 Years

Maximum Eligible Age

40 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Fundação de Amparo à Pesquisa do Estado de São Paulo

OTHER_GOV

Sponsor Role collaborator

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.

OTHER_GOV

Sponsor Role collaborator

Instituto Colombiano para el Desarrollo de la Ciencia y la Tecnología (COLCIENCIAS)

OTHER_GOV

Sponsor Role collaborator

University of Sao Paulo

OTHER

Sponsor Role lead

Responsible Party

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Ellen Cristini de Freitas

Principal Investigator

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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University of São Paulo, School of Physical Education and Sports of Ribeirão Preto.

Ribeirão Preto, São Paulo, Brazil

Site Status

Countries

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Brazil

References

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Martinez Galan BS, Giolo De Carvalho F, Carvalho SCS, Cunha Brandao CF, Morhy Terrazas SI, Abud GF, Meirelles MSS, Sakagute S, Ueta Ortiz G, Marchini JS, Aristizabal JC, Cristini de Freitas E. Casein and Whey Protein in the Breast Milk Ratio: Could It Promote Protein Metabolism Enhancement in Physically Active Adults? Nutrients. 2021 Jun 23;13(7):2153. doi: 10.3390/nu13072153.

Reference Type DERIVED
PMID: 34201617 (View on PubMed)

Other Identifiers

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Protein Supplementation

Identifier Type: -

Identifier Source: org_study_id