Protein Ingestion Timing on Body Composition and Biochemical Markers in Resistance-trained Males

NCT ID: NCT05544955

Last Updated: 2025-06-11

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 40 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2022-09-17
• Study Completion Date: 2022-12-31

Brief Summary

Protein ingestion timing is an important component for muscle accretion. The investigators hypothesized that protein ingestion timing could affect muscular adaptations and performance in resistance-trained men.

Conditions

Hypertrophy; Physical Performance

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: TRIPLE

Study Groups

Protein ingestion immediately prior to and immediately after resistance training

This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Group Type: EXPERIMENTAL

isolate whey protein prior to and immediately after resistance training

Intervention Type: DIETARY_SUPPLEMENT

This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Protein ingestion 3 hours prior to and 3 hours after resistance training

This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Group Type: EXPERIMENTAL

isolate whey protein 3 hours prior to and 3 hours after resistance training

Intervention Type: DIETARY_SUPPLEMENT

This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Interventions

isolate whey protein prior to and immediately after resistance training

This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Intervention Type: DIETARY_SUPPLEMENT

isolate whey protein 3 hours prior to and 3 hours after resistance training

This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.

Intervention Type: DIETARY_SUPPLEMENT

Eligibility Criteria

Inclusion Criteria

• performing resistance training at least three times a week for 1 year prior to the start of the study
• not taking any steroids or any illegal agents known to increase muscle size for at least 1 year prior to the start of the study
• no known medical issues
• sleeping for at least seven to eight hours during the 24-hour day

Exclusion Criteria

• non-willingness to continue protein ingestion or exercise protocols
• participation in exercise other than the prescribed resistance training program
• consumption of dietary supplement (other than the protein intervention) during the study period
• Missing more than one resistance training session or protein ingestion throughout the study period

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 35 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

Shahid Beheshti University of Medical Sciences

OTHER

Sponsor Role: collaborator

Marymount University

OTHER

Sponsor Role: lead

Responsible Party

Alexei Wong

Associate Professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Locations

Marymount University

Arlington, Virginia, United States

Countries

United States

Other Identifiers

IR.SSRC.REC.1403.059

Identifier Type: -

Identifier Source: org_study_id