Consistency Between Treatment Responses in PDO Models and Clinical Outcomes in Gastric Cancer

NCT ID: NCT05203549

Last Updated: 2022-01-24

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 250 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2021-05-01
• Study Completion Date: 2023-06-30

Brief Summary

Gastric cancer is the fourth leading cause of cancer-related death worldwide. Accurate assessment of the clinical responses to current treatment regimens is key to improving the prognosis and prolonging the survival of patients. In this study, two hundred and fifty patients with gastric cancer who ought to receive neoadjuvant therapy, conversion therapy or palliative chemotherapy will be enrolled, and patient-derived organoids from their tumor biopsies will be used to test the sensitivity of chemotherapy drugs which mainly include 5-fluorouracil, irinotecan, oxaliplatin and paclitaxel.

Detailed Description

Two hundred and fifty stage II-IV gastric cancer patients who should receive neoadjuvant therapy, conversion therapy or palliative chemotherapy will be enrolled in this study. Baseline information of the enrolled patients including medical history, physical examination records and clinical examination records will be collected. Tumor material of those patients will be obtained from gastric endoscopic biopsies or surgical resection. Patient-derived organoids (PDOs) will be established and cultured from gastric cancer tumor specimens. PDOs will then be treated with drugs of the chemotherapeutic regimens for gastric cancer. Organoid size and growth will be monitored before and after the treatment, and dose-response curves will be generated. As for the assessment of clinical outcomes of patients, tumor regression grade (TRG) systems will be used to evaluate tumor histological responses, and treatment responses will also be assessed by biomedical imaging according to the Response Evaluation Criteria in Solid Tumors (RECIST1.1). Consistency between treatment responses in PDO models and clinical outcomes of patients will be assessed by correlation analysis.

Conditions

Gastric Cancer

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

Gastric cancer patients

Patients with gastric cancer will undergo tumor biopsy before receiving neoadjuvant therapy, conversion therapy, or palliative therapy

Tumor biopsy

Intervention Type: PROCEDURE

Patients with gastric cancer will undergo tumor biopsy before receiving neoadjuvant therapy, conversion therapy, or palliative therapy

Interventions

Tumor biopsy

Patients with gastric cancer will undergo tumor biopsy before receiving neoadjuvant therapy, conversion therapy, or palliative therapy

Intervention Type: PROCEDURE

Eligibility Criteria

Inclusion Criteria

1. Age from 18 to 70 years old, no gender limit

2. The primary tumor is diagnosed as gastric adenocarcinoma (papillary, tubular, signet ring cell, poorly differentiated and mucinous adenocarcinoma) by endoscopic biopsy sampling and follow-up histopathological staining

3. The pre-operative staging includes: a) Stage IVa（cT4bNanyM0），resectable; b) Stage II-III（cT1-2N1-3M0、cT3-4aN0-3M0）, gastro-oesophageal junction (GEJ) cancer；c) Stage III（cT3-4aN1-3M0）, non-GEJ cancer, appropriate for neoadjuvant therapy

4. Stage IVb or locally unresectable late-stage gastric or GEJ cancer, previously never received chemotherapy

5. Eastern Cooperative Oncology Group (ECOG) performance score 0 or 1

6. The function of major organs is normal, meeting the following criteria: a) routine blood tests (no blood transfusion in the past 14 days): HB≥90g/L；ANC ≥1.5×109/L；PLT ≥80×109/L；b）blood biochemical parameters: BIL <1.5-fold upper limit of normal (ULN)；ALT<2.5×ULN; AST<2.5×ULN；Crea≤1×ULN

7. Fresh tumor tissue biopsies are obtainable and the clinical information of the patients is complete

8. Patients have been informed and consented

Exclusion Criteria

1. Previously received neoadjuvant therapy

2. Previously had other types of malignancy and received chemotherapy or radiotherapy

3. Previously had chicken pox, herpes zoster or other severe contagious diseases

4. Have severe active ulcer (gastrointestinal tract, skin, etc.) or have developed a high fever

5. Have a medical history of myocardial infarction, cerebral infarction, or pulmonary embolism

6. Have complications of gastric cancer (bleeding, perforation and obstruction etc.) and need emergency operation

7. Pregnant or lactating women

8. Have severe mental diseases

9. Allergic to chemotherapy drugs

10. Have metastatic brain cancer

11. Refuse to cooperate and to complete a treatment

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 70 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Shanghai Zhongshan Hospital

OTHER

Sponsor Role: collaborator

D1 Medical Technology (Shanghai) Co., Ltd, China

INDUSTRY

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Xuefei Wang, MD

Role: PRINCIPAL_INVESTIGATOR

Shanghai Zhongshan Hospital

Locations

Zhongshan Hospital Fudan University

Shanghai, Shanghai Municipality, China

Site Status: RECRUITING

Countries

China

Central Contacts

Hanqing Lin, PhD

Role: CONTACT

linhq@d1med.com

+8615921348040

Zhaoqing Tang, MD

Role: CONTACT

tang.zhaoqing@zs-hospital.sh.cn

+8613817125778

Facility Contacts

Xuefei Wang, MD

Role: primary

wang.xuefei@zs-hospital.sh.cn

+8613917270528

Other Identifiers

D1med-GC1001

Identifier Type: -

Identifier Source: org_study_id