Detection of Peritoneal Metastasis of Gastric Cancer by Liquid Biopsy in Peripheral Blood: A Prospective Study

NCT ID: NCT05347524

Last Updated: 2022-04-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

384 participants

Study Classification

OBSERVATIONAL

Study Start Date

2022-03-01

Study Completion Date

2030-06-30

Brief Summary

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This study is a prospective, multi-omics, observational study aimed at detecting peritoneal metastasis of gastric cancer by combined assays for methylation of cell-free DNA (cfDNA) and other blood-based biomarkers. The study will enroll 384 participants with gastric cancer.

Detailed Description

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Peritoneal metastasis (PM) in gastric cancer is associated with a poor prognosis.

Laparoscopy with cytology is performed to evaluate for peritoneal spread when considering chemoradiation or surgery. However, the laparoscopy is invasive and the sensitivity of computed tomography (CT) scan is poor for detecting PM. Therefore, it is necessary to evaluate the feasibility of cfDNA methylation and other blood-based biomarkers for the PM diagnosis.

The study will enroll 384 participants with gastric cancer. Baseline blood and diagnosis of PM by laparoscopy with cytology will be collected. Participants with PM will be defined as the case arm and participants without PM will be defined as the control arm. A PM diagnostic model will be developed.

Conditions

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Cancer

Study Design

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Observational Model Type

OTHER

Study Time Perspective

PROSPECTIVE

Study Groups

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Case arm - Gastric cancer with peritoneal metastasis

Baseline blood samples will be collected from gastric cancer participants with peritoneal metastasis.

Baseline blood draw and blood-based biomarkers analyses

Intervention Type OTHER

Baseline blood draw and blood-based biomarkers analyses

Control arm - Gastric cancer without peritoneal metastasis

Baseline blood samples will be collected from gastric cancer participants without peritoneal metastasis.

Baseline blood draw and blood-based biomarkers analyses

Intervention Type OTHER

Baseline blood draw and blood-based biomarkers analyses

Interventions

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Baseline blood draw and blood-based biomarkers analyses

Baseline blood draw and blood-based biomarkers analyses

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

1. Age 18-74 years at the day of consenting to the study.
2. Able to provide a written informed consent.
3. No prior cancer treatment (local or systematic) with either of the following:

A. Pathologically confirmed gastric cancer diagnosis within 42 days prior to blood draw.

B. High suspicious for cancer diagnosis by imaging tests or other routine clinical examinations, with confirmed pathological cancer diagnosis within 42 days after the blood draw.
4. Diagnosis of peritoneal metastasis by laparoscopy with cytology.

1. Age 18-74 years at the day of consenting to the study.
2. Able to provide a written informed consent.
3. No prior cancer treatment (local or systematic) with either of the following:

A. Pathologically confirmed gastric cancer diagnosis within 42 days prior to blood draw.

B. High suspicious for cancer diagnosis by imaging tests or other routine clinical examinations, with confirmed pathological cancer diagnosis within 42 days after the blood draw.
4. No peritoneal metastasis detected by laparoscopy with cytology.

Exclusion Criteria

1. Insufficient qualified blood samples.
2. During pregnancy or lactation.
3. Recipient of organ transplant or prior non-autologous (allogeneic) bone marrow or stem cell transplant.
4. Recipient of blood transfusion within 7 days prior to blood draw.
5. Recipient of anti-tumor drugs to treat non-cancer diseases within 30 days prior to blood draw.
6. With other known malignant tumors or multiple primary tumors.

1. Insufficient qualified blood samples.
2. During pregnancy or lactation.
3. Recipient of organ transplant or prior non-autologous (allogeneic) bone marrow or stem cell transplant.
4. Recipient of blood transfusion within 7 days prior to blood draw.
5. Recipient of anti-tumor drugs to treat non-cancer diseases within 30 days prior to blood draw.
6. With other known malignant tumors or multiple primary tumors.
Minimum Eligible Age

18 Years

Maximum Eligible Age

74 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Guangzhou Burning Rock Bioengineering Ltd.

INDUSTRY

Sponsor Role collaborator

Shanghai Zhongshan Hospital

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Yihong Sun, Ph. D

Role: PRINCIPAL_INVESTIGATOR

Zhongshan Hospital, Fudan University, Shanghai, China

Xuefei Wang, Ph. D

Role: PRINCIPAL_INVESTIGATOR

Zhongshan Hospital, Fudan University, Shanghai, China

Locations

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ZhongShan Hospital, Fudan university, Shanghai, China

Shanghai, Shanghai Municipality, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Yihong Sun, Ph. D

Role: CONTACT

+86-021-65642662

Xuefei Wang, Ph. D

Role: CONTACT

+86-021-65642662

Facility Contacts

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Yihong Sun, MD

Role: primary

86-13701735406

References

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Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ, He J. Cancer statistics in China, 2015. CA Cancer J Clin. 2016 Mar-Apr;66(2):115-32. doi: 10.3322/caac.21338. Epub 2016 Jan 25.

Reference Type BACKGROUND
PMID: 26808342 (View on PubMed)

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018 Nov;68(6):394-424. doi: 10.3322/caac.21492. Epub 2018 Sep 12.

Reference Type BACKGROUND
PMID: 30207593 (View on PubMed)

Thomassen I, van Gestel YR, van Ramshorst B, Luyer MD, Bosscha K, Nienhuijs SW, Lemmens VE, de Hingh IH. Peritoneal carcinomatosis of gastric origin: a population-based study on incidence, survival and risk factors. Int J Cancer. 2014 Feb 1;134(3):622-8. doi: 10.1002/ijc.28373. Epub 2013 Aug 5.

Reference Type BACKGROUND
PMID: 23832847 (View on PubMed)

Allen CJ, Newhook TE, Vreeland TJ, Das P, Minsky BD, Blum M, Song S, Ajani J, Ikoma N, Mansfield PF, Roy-Chowdhuri S, Badgwell BD. Yield of peritoneal cytology in staging patients with gastric and gastroesophageal cancer. J Surg Oncol. 2019 Dec;120(8):1350-1357. doi: 10.1002/jso.25729. Epub 2019 Oct 14.

Reference Type BACKGROUND
PMID: 31612494 (View on PubMed)

Chen M, Zhao H. Next-generation sequencing in liquid biopsy: cancer screening and early detection. Hum Genomics. 2019 Aug 1;13(1):34. doi: 10.1186/s40246-019-0220-8.

Reference Type BACKGROUND
PMID: 31370908 (View on PubMed)

Kinoshita J, Fushida S, Harada S, Makino I, Nakamura K, Oyama K, Fujita H, Ninomiya I, Fujimura T, Kayahara M, Ohta T. Type IV collagen levels are elevated in the serum of patients with peritoneal dissemination of gastric cancer. Oncol Lett. 2010 Nov;1(6):989-994. doi: 10.3892/ol.2010.181. Epub 2010 Sep 23.

Reference Type BACKGROUND
PMID: 22870099 (View on PubMed)

So JBY, Kapoor R, Zhu F, Koh C, Zhou L, Zou R, Tang YC, Goo PCK, Rha SY, Chung HC, Yoong J, Yap CT, Rao J, Chia CK, Tsao S, Shabbir A, Lee J, Lam KP, Hartman M, Yong WP, Too HP, Yeoh KG. Development and validation of a serum microRNA biomarker panel for detecting gastric cancer in a high-risk population. Gut. 2021 May;70(5):829-837. doi: 10.1136/gutjnl-2020-322065. Epub 2020 Oct 7.

Reference Type BACKGROUND
PMID: 33028667 (View on PubMed)

Shimura T, Toden S, Kandimalla R, Toiyama Y, Okugawa Y, Kanda M, Baba H, Kodera Y, Kusunoki M, Goel A. Genomewide Expression Profiling Identifies a Novel miRNA-based Signature for the Detection of Peritoneal Metastasis in Patients With Gastric Cancer. Ann Surg. 2021 Nov 1;274(5):e425-e434. doi: 10.1097/SLA.0000000000003647.

Reference Type BACKGROUND
PMID: 31663973 (View on PubMed)

Haber DA, Velculescu VE. Blood-based analyses of cancer: circulating tumor cells and circulating tumor DNA. Cancer Discov. 2014 Jun;4(6):650-61. doi: 10.1158/2159-8290.CD-13-1014. Epub 2014 May 6.

Reference Type BACKGROUND
PMID: 24801577 (View on PubMed)

Pantel K, Alix-Panabieres C. Liquid biopsy and minimal residual disease - latest advances and implications for cure. Nat Rev Clin Oncol. 2019 Jul;16(7):409-424. doi: 10.1038/s41571-019-0187-3.

Reference Type BACKGROUND
PMID: 30796368 (View on PubMed)

Qi C, Li P, Hu Y, et al. The ctDNA in peritoneal effusion of advanced gastric cancer for auxiliary diagnosis of peritoneal metastasis. Journal of Clinical Oncology. 2019/05/20 2019;37(15_suppl):e15516-e15516. doi:10.1200/JCO.2019.37.15_suppl.e15516

Reference Type BACKGROUND

Wu R, Li Q, Wu F, Shi C, Chen Q. Comprehensive Analysis of CDC27 Related to Peritoneal Metastasis by Whole Exome Sequencing in Gastric Cancer. Onco Targets Ther. 2020 Apr 21;13:3335-3346. doi: 10.2147/OTT.S244351. eCollection 2020.

Reference Type BACKGROUND
PMID: 32368092 (View on PubMed)

Zhao D, Yue P, Wang T, Wang P, Song Q, Wang J, Jiao Y. Personalized analysis of minimal residual cancer cells in peritoneal lavage fluid predicts peritoneal dissemination of gastric cancer. J Hematol Oncol. 2021 Oct 12;14(1):164. doi: 10.1186/s13045-021-01175-2.

Reference Type BACKGROUND
PMID: 34641926 (View on PubMed)

Klutstein M, Nejman D, Greenfield R, Cedar H. DNA Methylation in Cancer and Aging. Cancer Res. 2016 Jun 15;76(12):3446-50. doi: 10.1158/0008-5472.CAN-15-3278. Epub 2016 Jun 2.

Reference Type BACKGROUND
PMID: 27256564 (View on PubMed)

Meng H, Cao Y, Qin J, Song X, Zhang Q, Shi Y, Cao L. DNA methylation, its mediators and genome integrity. Int J Biol Sci. 2015 Apr 8;11(5):604-17. doi: 10.7150/ijbs.11218. eCollection 2015.

Reference Type BACKGROUND
PMID: 25892967 (View on PubMed)

Morgan AE, Davies TJ, Mc Auley MT. The role of DNA methylation in ageing and cancer. Proc Nutr Soc. 2018 Nov;77(4):412-422. doi: 10.1017/S0029665118000150. Epub 2018 Apr 30.

Reference Type BACKGROUND
PMID: 29708096 (View on PubMed)

Widschwendter M, Jones A, Evans I, Reisel D, Dillner J, Sundstrom K, Steyerberg EW, Vergouwe Y, Wegwarth O, Rebitschek FG, Siebert U, Sroczynski G, de Beaufort ID, Bolt I, Cibula D, Zikan M, Bjorge L, Colombo N, Harbeck N, Dudbridge F, Tasse AM, Knoppers BM, Joly Y, Teschendorff AE, Pashayan N; FORECEE (4C) Consortium. Epigenome-based cancer risk prediction: rationale, opportunities and challenges. Nat Rev Clin Oncol. 2018 May;15(5):292-309. doi: 10.1038/nrclinonc.2018.30. Epub 2018 Feb 27.

Reference Type BACKGROUND
PMID: 29485132 (View on PubMed)

Guo S, Diep D, Plongthongkum N, Fung HL, Zhang K, Zhang K. Identification of methylation haplotype blocks aids in deconvolution of heterogeneous tissue samples and tumor tissue-of-origin mapping from plasma DNA. Nat Genet. 2017 Apr;49(4):635-642. doi: 10.1038/ng.3805. Epub 2017 Mar 6.

Reference Type BACKGROUND
PMID: 28263317 (View on PubMed)

Liu MC, Oxnard GR, Klein EA, Swanton C, Seiden MV; CCGA Consortium. Sensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNA. Ann Oncol. 2020 Jun;31(6):745-759. doi: 10.1016/j.annonc.2020.02.011. Epub 2020 Mar 30.

Reference Type BACKGROUND
PMID: 33506766 (View on PubMed)

Harada H, Soeno T, Nishizawa N, Washio M, Sakuraya M, Ushiku H, Niihara M, Hosoda K, Kumamoto Y, Naitoh T, Sangai T, Hiki N, Yamashita K. Prospective study to validate the clinical utility of DNA diagnosis of peritoneal fluid cytology test in gastric cancer. Cancer Sci. 2021 Apr;112(4):1644-1654. doi: 10.1111/cas.14850. Epub 2021 Feb 27.

Reference Type BACKGROUND
PMID: 33576114 (View on PubMed)

Hu XY, Ling ZN, Hong LL, Yu QM, Li P, Ling ZQ. Circulating methylated THBS1 DNAs as a novel marker for predicting peritoneal dissemination in gastric cancer. J Clin Lab Anal. 2021 Sep;35(9):e23936. doi: 10.1002/jcla.23936. Epub 2021 Aug 13.

Reference Type BACKGROUND
PMID: 34390026 (View on PubMed)

Juzenas S, Venkatesh G, Hubenthal M, Hoeppner MP, Du ZG, Paulsen M, Rosenstiel P, Senger P, Hofmann-Apitius M, Keller A, Kupcinskas L, Franke A, Hemmrich-Stanisak G. A comprehensive, cell specific microRNA catalogue of human peripheral blood. Nucleic Acids Res. 2017 Sep 19;45(16):9290-9301. doi: 10.1093/nar/gkx706.

Reference Type BACKGROUND
PMID: 28934507 (View on PubMed)

Rijken A, Lurvink RJ, Luyer MDP, Nieuwenhuijzen GAP, van Erning FN, van Sandick JW, de Hingh IHJT. The Burden of Peritoneal Metastases from Gastric Cancer: A Systematic Review on the Incidence, Risk Factors and Survival. J Clin Med. 2021 Oct 23;10(21):4882. doi: 10.3390/jcm10214882.

Reference Type BACKGROUND
PMID: 34768402 (View on PubMed)

Other Identifiers

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B2022-081R

Identifier Type: -

Identifier Source: org_study_id

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