Cetuximab Sensitivity Correlation Between Patient-Derived Organoids and Clinical Response in Colon Cancer Patients.

NCT ID: NCT04906733

Last Updated: 2021-08-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

80 participants

Study Classification

OBSERVATIONAL

Study Start Date

2021-04-15

Study Completion Date

2023-12-31

Brief Summary

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Eighty patients with RAS/RAF wild-type metastatic right colon cancer will be enrolled and undergo a fresh biopsy of tumor lesion before the standard treatment of chemotherapy. The investigators will establish organoids from the pre-treatment biopsies. Organoids will be exposed to the chemotherapy drugs or chemotherapy drugs combined with cetuximab used for each patient. The sensitivity of chemotherapy drugs or combined cetuximab will be tested in the organoids model. Chemotherapy strategies including 5-fluorouracil only, irinotecan only, oxaliplatin only, FOLFOX, and FOLFIRI. The purpose of this study is to evaluate the consistency and accuracy of a Patient-Derived Organoid (PDO) model of colon cancer to predict the clinical efficacy of combined treatment of cetuximab, which to formulate the best therapy regimen for each given patient.

Detailed Description

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Investigators hypothesize that the cetuximab sensitivity test results on patient-derived organoids can be used to predict the treatment selection of patients with RAS/RAF wild-type metastatic right colon cancer.

Objectives

1. To assess the effective rate of cetuximab target therapy on RAS/RAF wild-type right colon cancer
2. To assess the consistency of organoid chemotherapy sensitivity and clinical chemotherapy efficacy
3. To assess the consistency of organoid cetuximab sensitivity and clinical cetuximab efficacy

The following points will be addressed:

1. The biopsy tissue of RAS/RAF wild-type metastatic right colon cancer will be collected and subject to ex vivo 3-D culture to establish patient-derived tumor organoids, which will be used for the drug sensitivity test.
2. Enrolled patients will treat with chemotherapy or chemotherapy combined target therapy. The medication regime and treatment cycle will be decided based on the clinical guideline and evidence-based medicine.
3. The patient-derived organoid-based drug sensitivity test will compare with clinical treatment data of chemotherapy or chemotherapy combined target therapy, followed by correlation analysis.

Conditions

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Colon Cancer Organoids Metastatic Cancer

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Cancer patient

RAS/RAF wild-type metastatic right colon cancer patients receiving chemotherapy or chemotherapy combined target therapy treatment.

Tumor biopsy

Intervention Type PROCEDURE

Patients with RAS/RAF wild-type metastatic right colon cancer will receive biopsy before the standard treatment of chemotherapy or target therapy.

Interventions

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Tumor biopsy

Patients with RAS/RAF wild-type metastatic right colon cancer will receive biopsy before the standard treatment of chemotherapy or target therapy.

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

1. Age from 18 to 70 years old, no gender limit
2. The primary tumor is located in the right colon, including the ileocecal area, ascending colon, liver flexure, and the right part of the transverse colon.
3. Pathologically proved primary intestinal tumor with simultaneous / metachronous metastasis
4. Molecular pathology confirmed as RAS/RAF wild type
5. Can tolerate and cooperate with the completion of chemotherapy and targeted therapy
6. Tissues can be obtained through puncture or colonoscopy for organoid culture
7. Complete clinical data, as well as efficacy evaluation data, can be obtained

Exclusion Criteria

1. Less than 18 years of age or more than 70 years of age
2. The primary tumor is located in the left colon or rectum
3. Pathology results can't confirm as colon cancer
4. RAS and RAF are mutant confirmed by molecular pathology
5. Refuse to cooperate and complete the treatment, or there is a contraindication for chemotherapy or targeted therapy
6. No available metastatic lesions tissue
7. Unable to obtain complete clinical data or efficacy evaluation data
Minimum Eligible Age

18 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Fudan University

OTHER

Sponsor Role collaborator

D1 Medical Technology (Shanghai) Co., Ltd, China

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Junjie Peng, Dr.

Role: PRINCIPAL_INVESTIGATOR

Fudan University

Locations

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Fudan University Shanghai Cancer Center

Shanghai, Shanghai Municipality, China

Site Status RECRUITING

Countries

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China

Central Contacts

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Hanqing Lin, Dr.

Role: CONTACT

+8615921348040

Chunyan Cheng, Dr.

Role: CONTACT

+8613916290275

Facility Contacts

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Junjie Peng, Dr.

Role: primary

+8602164175590

Provided Documents

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Document Type: Informed Consent Form

View Document

Other Identifiers

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D1med-ZL01

Identifier Type: -

Identifier Source: org_study_id

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