Study on Antigen-presenting Function of Gamma Delta T Cells in Sepsis and Its Molecular Mechanisms

NCT ID: NCT03379896

Last Updated: 2017-12-22

Basic Information

• Recruitment Status: UNKNOWN
• Total Enrollment: 60 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2018-01-04
• Study Completion Date: 2020-12-30

Brief Summary

Study on antigen-presenting function of gamma delta T cells in sepsis and its molecular mechanisms

Detailed Description

The latest definition of sepsis highlights the role of dysregulated host response to infection in organ dysfunction aggravation. Maintenance of immune homeostasis of septic patients through immunotherapy is the key breakthrough to improve success rate. Antigen-presenting cell (APC) is the bridge that connects the innate and adaptive immunity. Decrease of count and function of APC has been shown to be associated with worsened clinical outcomes in patients with sepsis. Gamma-delta T cells (γδ T cells) are the newly identified population of T lymphocytes. Recent studies have found that γδ T cells possess unique and powerful antigen-presenting function, making them the hot topic in infection and cancer research. Based on the results, the investigator plan to evaluate the antigen-presenting function changes of γδ T cells in septic status by analyzing the antigen-presenting related molecules on γδ T cells, antigen protein uptake ability, and their function on the proliferation and activation of CD8+ T lymphocytes. Furthermore, the investigator will explore the mechanism and signal pathways for the APC function changes of γδ T cells. Findings from this research could help explain the mechanism of sepsis induced immune dysfunction, enrich our understanding of the important role of γδ T cells, and build a good foundation for immunotherapy in sepsis.

Conditions

Sepsis; Antigen-presenting Function; γδ T Cells

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

Sepsis

Patient diagnosed with sepsis according to the new sepsis definition (infection+SOFA≥2).

Collect peripheral blood sample

Intervention Type: OTHER

Collect 20 ml heparinized blood from patients and controls strictly abiding by the principle of aseptic manipulation after informed consent.

Control

Age-matched healthy control

Collect peripheral blood sample

Intervention Type: OTHER

Collect 20 ml heparinized blood from patients and controls strictly abiding by the principle of aseptic manipulation after informed consent.

Interventions

Collect peripheral blood sample

Collect 20 ml heparinized blood from patients and controls strictly abiding by the principle of aseptic manipulation after informed consent.

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

1. ≥18 year old;

2. a known or suspected infection based on clinical data at the time of admission and sepsis-induced dysfunction of at least one organ.

Exclusion Criteria

1. autoimmune disease,

2. history of transplantation,

3. acute tuberculosis,

4. chronic hepatitis B or C infection,

5. HIV infection,

6. active malignancy, and

7. long-term use of cortical steroids.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 80 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

West China Hospital

OTHER

Sponsor Role: lead

Responsible Party

Kang Yan

Yan Kang, Director of Department of Critical Care Medicine

Responsibility Role: PRINCIPAL_INVESTIGATOR

Central Contacts

Xuelian Liao, MD

Role: CONTACT

xuelianliao@hotmail.com

8613541023033

Other Identifiers

81701880

Identifier Type: -

Identifier Source: org_study_id