Muscle Protein Synthesis After Whole Egg vs. Egg White Consumption

NCT ID: NCT03117127

Last Updated: 2018-05-01

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 10 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2015-07-31
• Study Completion Date: 2017-05-31

Brief Summary

In crossover trials, ten (N=10) young men (18-35 y) will receive stable isotope tracer infusions and perform a single bout of resistance exercise. Immediately after resistance exercise, participants will ingest stable isotope labeled whole eggs (18 g protein, 17 g fat) or egg whites (18 g protein, 0 g fat) cooked in scrambled form. Repeated blood and muscle biopsies will be collected to determine whole body amino acid kinetics, muscle amino acid transporters, anabolic signaling and myofibrillar protein synthesis rates during the trials.

Detailed Description

On both infusion trials, participants will report to the laboratory at 0700 h after an overnight fast. Upon arrival to the lab, a baseline breath sample will be collected for determination of 13CO2 enrichment by isotope ratio mass spectrometry. A Teflon catheter will be inserted into an antecubital vein for baseline blood sample collection (t=-210 min) and then participants will receive priming doses of NaH13CO2 (2.35 µmol·kg-1), L-[1-13C]leucine (7.6 µmol·kg-1), and L-[ring-2H5]phenylalanine (2.0 µmol·kg-1). Subsequently, a continuous intravenous infusion of L-[1-13C]leucine (0.10 µmol·kg-1·min-1) and L-[ring-2H5]phenylalanine (0.05 µmol·kg-1·min-1) will be initiated (t=-210 min) and maintained over the infusion trials. A second Teflon catheter willinserted into a heated dorsal hand vein for repeated arterialized blood sampling and remained patent by a 0.9% saline drip. Breath samples and arterialized blood samples and will be collected every 30 or 60 min during the postabsorptive- and postprandial states. In the post-absorptive state of infusion trial 1, muscle biopsies will be collected at t=-150 and -30 min of infusion to determine basal-state myofibrillar protein synthesis rates, relative skeletal muscle amino acid transporter content, and anabolic-related signaling. In the subsequent cross-over trial only 1 muscle biopsy will be collected at t=-30 for Western blot analysis and postabsorptive myofibrillar protein-bound tracer enrichment. After collection of the resting muscle biopsy at t=-30 for both trials, the participants will perform resistance exercise that consists of 4 sets of 10 repetitions at 80% of 10-RM for both leg press and leg extension exercise.

Immediately after completion of the exercise bout, participants will consume 3 whole eggs or an equivalent amount of protein from egg whites (t=0 min). Completion of the meal will mark the start of the postprandial phase (t=0 min) and additional muscle biopsies will be collected at t=120 and 300 min.

Conditions

Hypertrophy

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: SINGLE

Study Groups

Whole eggs

After resistance exercise, participants will ingest whole eggs (18 g protein, 17 g fat) cooked in scrambled form.

Group Type: ACTIVE_COMPARATOR

Resistance Exercise

Intervention Type: BEHAVIORAL

Participants will perform leg extension and leg press immediately prior to ingestion of whole eggs or egg whites

Whole Eggs

Intervention Type: OTHER

Participants will ingestion whole eggs immediately after resistance exercise

Egg Whites

After resistance exercise, participants will ingest egg whites (18 g protein, 0 g fat) cooked in scrambled form.

Group Type: EXPERIMENTAL

Resistance Exercise

Intervention Type: BEHAVIORAL

Participants will perform leg extension and leg press immediately prior to ingestion of whole eggs or egg whites

Egg Whites

Intervention Type: OTHER

Participants will ingestion whole eggs immediately after resistance exercise

Interventions

Resistance Exercise

Participants will perform leg extension and leg press immediately prior to ingestion of whole eggs or egg whites

Intervention Type: BEHAVIORAL

Whole Eggs

Participants will ingestion whole eggs immediately after resistance exercise

Intervention Type: OTHER

Egg Whites

Participants will ingestion whole eggs immediately after resistance exercise

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Male
• Aged 18-35 years
• Healthy, active (self-reported to exercise 2 - 4 times per week)
• BMI > 18.5 and < 25 kg/m2

Exclusion Criteria

• Smoking
• Known allergies to egg consumption
• Vegans
• Diagnosed GI tract diseases
• Arthritic conditions
• A history of neuromuscular problems
• Diagnosed cognitive impairments
• Recent (1 year) participation in amino acid tracer studies
• Predisposition to hypertrophic scarring or keloid formation
• Individuals on any medications known to affect protein metabolism (i.e. corticosteroids, non-steroidal anti-inflammatories, or prescription strength acne medications).
• High blood pressure (Systolic > 140 mm HG; Diastolic > 90 mm HG)

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 35 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

University of Illinois at Urbana-Champaign

OTHER

Sponsor Role: lead

Responsible Party

Nicholas Burd

Assistant Professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Nicholas A Burd, Ph.D.

Role: PRINCIPAL_INVESTIGATOR

Assistant Professor

Locations

Freer Hall

Urbana, Illinois, United States

Countries

United States

References

van Vliet S, Shy EL, Abou Sawan S, Beals JW, West DW, Skinner SK, Ulanov AV, Li Z, Paluska SA, Parsons CM, Moore DR, Burd NA. Consumption of whole eggs promotes greater stimulation of postexercise muscle protein synthesis than consumption of isonitrogenous amounts of egg whites in young men. Am J Clin Nutr. 2017 Dec;106(6):1401-1412. doi: 10.3945/ajcn.117.159855. Epub 2017 Oct 4.

Reference Type: DERIVED

PMID: 28978542 (View on PubMed)

Other Identifiers

15693

Identifier Type: -

Identifier Source: org_study_id