Effects on Immun System of Anesthesia

NCT ID: NCT02056561

Last Updated: 2014-02-06

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 45 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2010-01-31
• Study Completion Date: 2011-01-31

Brief Summary

Introduction and Aim: Desflurane and sevoflurane are frequently used for maintenance of anesthesia and studies have shown that these anesthetics cause a variety of changes in oxidative stress and antioxidative defense mechanisms. The chemical structure of propofol is similar to some free radical consumers such as endogenous vitamin E and butylated hydroxene toluene. This study aims to compare the effects of sevoflurane, desflurane and propofol infusion anesthesia on the oxidant and antioxidant systems of patients undergoing laparoscopic cholecystectomy.

Material and Method: Forty-five patients, ASA (American Society of Anesthesiologists) I-II, score between 18-50 years with planned laparoscopic cholecystectomy under general anesthesia were included in the study. Patients were divided into 3 groups as total intravenous anesthesia (TIVA) (group T n: 15), sevoflurane (group S n: 15) and desflurane (group D n: 15). Propofol 2 mg/kg IV, fentanyl 1 mcg/kg IV, vecuronium 0.1 mg/kg IV given to all groups for anesthesia induction. For maintenance of anesthesia group S were ventilated with 2% sevoflurane, 50% air and 50% O2 mix at 6 L/min flow. Group D were given 6% desflurane 50% air and 50% O2 mix at 6 L/min flow. Group T were given propofol infusions of 12 mg/kg/hr for the first 10 minutes, 9 mg/kg/hr for the second 10 minutes and 6 mg/kg/hr after that. Patients were ventilated with 50% air and 50% O2 mix at 6 L/min flow. Before induction and after the operation venous blood samples were taken to evaluate the levels of glutathion peroxidase, total oxidants and antioxidants.

Conditions

Anesthesia

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: PROSPECTIVE

Study Groups

TIVA (group T n: 15)

Group T were given propofol infusions of 12 mg/kg/hr for the first 10 minutes, 9 mg/kg/hr for the second 10 minutes and 6 mg/kg/hr after that. Patients were ventilated with 50% air and 50% O2 mix at 6 L/min flow.

No interventions assigned to this group

Sevoflurane (group S n: 15)

Group S were ventilated with 2% sevoflurane, 50% air and 50% O2 mix at 6 L/min flow.

No interventions assigned to this group

Desflurane (group D n: 15)

Group D cases were given 6% desflurane 50% air and 50% O2 mix at 6 L/min flow.

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• ASA I-II with patients
• Between 18-50 years
• Planned laparoscopic cholecystectomy under general anesthetic

Exclusion Criteria

• Patients with endocrine dysfunction
• blood transfusion in the previous 2 weeks
• signs of infection and inflammation
• history of preoperative medication use
• anemia
• hemorrhage requiring transfusion during the operation were excluded from the study.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 50 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Duzce University

OTHER

Sponsor Role: lead

Responsible Party

yavuz demiraran

Professor Doctor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

yavuz demiraran, MD prof

Role: PRINCIPAL_INVESTIGATOR

duzce university medical faculty

Locations

Duzce Univercity Medical Fauculty

Düzce, , Turkey (Türkiye)

Countries

Turkey (Türkiye)

Other Identifiers

MESUT-81

Identifier Type: -

Identifier Source: org_study_id