Exercise-induced Changes in DNA-methylation

NCT ID: NCT01931072

Last Updated: 2019-05-20

Basic Information

• Recruitment Status: TERMINATED
• Clinical Phase: NA
• Total Enrollment: 37 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2012-08-31
• Study Completion Date: 2013-12-31

Brief Summary

The purpose of the study was to investigate whether regular moderate- or high-intensity interval training can change the DNA methylation in a long-lasting state, and whether these changes affect the gene expression. In addition, the investigators will determine whether the intensity of the training could be an affecting factor of the possible DNA-methylation change as well. The null hypothesis was that no such exercise-induced changes occur.

Conditions

Healthy

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

High-intensity interval training

The High-intensity interval training group was instructed to complete exercise sessions of 4x4-minutes intervals at 85-95% of maximal heart rate, 3 times a week for 8 weeks. This type of exercise include 10 minutes warm-up, followed by four intervals of four minutes high-intensity (85-95% of HRmax), with three minutes active breaks (70% of HRmax) between each interval, and ending with seven minutes cool-down. An exercise session last for 42 minutes, where the participants should breathe heavily and feel exhausted four times.

Group Type: EXPERIMENTAL

High-intensity interval training

Intervention Type: BEHAVIORAL

Interval training 3 times a week for 8 weeks

Moderate training

The moderate training group was instructed to complete exercise sessions of 50 minutes at 70% of maximal heart rate, 3 times a week for 8 weeks.

Group Type: ACTIVE_COMPARATOR

Moderate training

Intervention Type: BEHAVIORAL

Moderate training 3 times a week for 8 weeks

Control

The control group followed the baseline and follow-up tests, and was asked to live normally and not change their dietary pattern or exercise habits during their participation in this project. Based on the well-known beneficial effects of exercise on general health, it was regarded unethical to demand the control group to desist from any types of physical activity during the project period. They got no further follow-up on exercise.

Group Type: NO_INTERVENTION

No interventions assigned to this group

Interventions

High-intensity interval training

Interval training 3 times a week for 8 weeks

Intervention Type: BEHAVIORAL

Moderate training

Moderate training 3 times a week for 8 weeks

Intervention Type: BEHAVIORAL

Eligibility Criteria

Inclusion Criteria

• Male
• 70-75 years old
• Signed written informed consent

Exclusion Criteria

• Incompatible with exercise
• Known cardiac disease
• Active cancer
• Significant pulmonary disease
• Uncontrolled hypertension
• Use of blood thinning medicine (except Albyl-E)
• Drug abuse
• Alcohol abuse
• Finishing less than 80% of the exercise sessions (this complies to the training groups)

• Minimum Eligible Age: 70 Years
• Maximum Eligible Age: 75 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

St. Olavs Hospital

OTHER

Sponsor Role: collaborator

Norwegian University of Science and Technology

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Ulrik Wisløff, Professor

Role: STUDY_DIRECTOR

National Taiwan Normal University

Locations

Institute for Circulation and Medical Imaging, NTNU

Trondheim, , Norway

Countries

Norway

Other Identifiers

EPI-GEN100

Identifier Type: -

Identifier Source: org_study_id