miRNA Machinery in Melanoma, Melanoma Metastases and Benign Melanocytic Naevi

NCT ID: NCT01444560

Last Updated: 2012-10-16

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 21 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2008-12-31
• Study Completion Date: 2011-09-30

Brief Summary

MicroRNAs (miRNAs) are very small endogenous RNA molecules about 22-25 nucleotides in length, capable of post-transcriptional gene regulation. miRNAs bind to their target messenger RNAs (mRNAs), leading to cleavage or suppression of target mRNA translation based on the degree of complementarity. miRNAs have recently been shown to play pivotal roles in diverse developmental and cellular processes and linked to a variety of skin diseases and cancers. In the present study, the investigators examines the expression profiles of miRNA machinery components such as miRNA maturation and transport factors, microprocessor complex and RISC subunits in cutaneous melanoma, cutaneous melanoma metastases and benign melanocytic nevi.

Detailed Description

Patients with cutaneous melanoma, cutaneous melanoma metastases and benign melanocytic nevi were included in the study. Punch biopsies were harvested from the center of the tumors. The miRNA machinery components were detected by quantitative real-time reverse transcriptase polymerase chain reaction.

Conditions

Cutaneous Melanoma; Nevi

Study Design

• Observational Model Type: COHORT
• Study Time Perspective: PROSPECTIVE

Study Groups

Cutaneous Melanoma

No interventions assigned to this group

Cutaneous Melanoma Metastases

No interventions assigned to this group

Benign Melanocytic Nevi

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

• cutaneous melanoma
• cutaneous melanoma metastases
• benign melanocytic nevi

Exclusion Criteria

• other forms of malignancy

• Minimum Eligible Age: 1 Year
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Ruhr University of Bochum

OTHER

Sponsor Role: lead

Responsible Party

Michael Sand

Postdoc

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Falk G Bechara, PD Dr.

Role: STUDY_DIRECTOR

Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum

Locations

Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum

Bochum, North Rhine-Westphalia, Germany

Countries

Germany

References

Sand M, Skrygan M, Georgas D, Sand D, Gambichler T, Altmeyer P, Bechara FG. The miRNA machinery in primary cutaneous malignant melanoma, cutaneous malignant melanoma metastases and benign melanocytic nevi. Cell Tissue Res. 2012 Oct;350(1):119-26. doi: 10.1007/s00441-012-1446-0. Epub 2012 Jun 17.

Reference Type: RESULT

PMID: 22706980 (View on PubMed)

Other Identifiers

microRNA4

Identifier Type: -

Identifier Source: org_study_id