The Application of DNA Nanomachines for Detecting microRNA in Blood for the Diagnosis of Pancreatic Cancer. Diagnosis of Pancreatic Cancer

NCT ID: NCT06497777

Last Updated: 2024-07-12

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

30 participants

Study Classification

OBSERVATIONAL

Study Start Date

2024-07-06

Study Completion Date

2026-06-01

Brief Summary

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Previous research has shown that microRNAs in the blood can serve as biomarkers for early pancreatic cancer, with potential applications including detection, differential diagnosis, and prognosis prediction of pancreatic cancer. The current primary method for detecting microRNAs is RT-qPCR, but this process requires repeated temperature cycling, which demands high precision from the equipment. As an alternative, isothermal nucleic acid amplification technology does not require expensive temperature control instruments. Our research team has developed various isothermal nucleic acid amplification strategies for microRNA sensing platforms, applied to biological sample detection. This study combines the circular strand displacement amplification strategy with DNA nanomachines to develop a fluorescence sensing platform that performs dual signal amplification at a constant temperature. It is designed to detect pancreatic cancer-related microRNAs, exploring its role and potential applications in the diagnosis of pancreatic cancer patients.

Detailed Description

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Conditions

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Pancreas Adenocarcinoma MicroRNA

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

PROSPECTIVE

Study Groups

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Pancreatic adenocarcinoma

Pancreatic adenocarcinoma which are proven by pathology, before initiation of anti-cancer treatment.

MicroRNA (mir-642b-3p)

Intervention Type GENETIC

A circulating microRNA in the blood of pancreatic cancer patients.

Healthy control

People who do not have pancreatic disease.

No interventions assigned to this group

Interventions

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MicroRNA (mir-642b-3p)

A circulating microRNA in the blood of pancreatic cancer patients.

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

* Pancreatic ductal adenocarcinoma
* Proven by pathology
* Patients who have not received anti-cancer therapies

Exclusion Criteria

* Less than 20 years old
* Unable to provide inform and consent
* Patients who have active malignancy other than pancreatic adenocarcinoma
* Patients who have had pancreatic cancer whose anti-cancer therapies are completed or undergoing
* Life expectancy less than 3 months
Minimum Eligible Age

20 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National Taiwan University Hospital

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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National Taiwan University Hospital

Taipei, , Taiwan

Site Status RECRUITING

Countries

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Taiwan

Central Contacts

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Weng Fai Wong, Master of Science

Role: CONTACT

886-972652272

Facility Contacts

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Weng Fai Wong, Master of Science

Role: primary

886-972652272

Other Identifiers

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202405082RINB

Identifier Type: -

Identifier Source: org_study_id

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