Cardio and Renovascular Effects of Isometric Exercise in the Healthy Human Measured by PET/CT and MRI

NCT ID: NCT06555211

Last Updated: 2024-08-15

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

10 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-05-07

Study Completion Date

2024-12-31

Brief Summary

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This randomized crossover study aims to validate measurements of renal perfusion in healthy males with PET/CT, using 15-oxygen labeled water as tracer, against the validated multiparametric MRI technique.

The study will determine:

* Total renal blood flow and regional renal perfusion at baseline (rest) and during isometric muscle exercise of the upper arm, using PET/CT (with 15-oxygen labeled water as a tracer) and MRI.
* The coefficient of variation of renal blood flow and regional renal perfusion measurements at baseline and during isometric muscle exercise of the upper arm, using PET/CT (with 15-oxygen labeled water as a tracer) and MRI.
* Regional renal oxygenation and oxygen extraction at baseline and during isometric muscle exercise of the upper arm using MRI.
* The coefficient of variation on regional renal oxygenation and oxygen extraction measurements at baseline and during isometric muscle exercise of the upper arm using MRI.

Detailed Description

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Measuring tissue perfusion with PET/CT using 15-oxygen labeled water is routinely used as a non-invasive tool to examine cerebral and myocardial perfusion. With a 100% tissue extraction, 15-oxygen labeled water is gold standard.

The aim of the present study is to validate renal perfusion measurements with PET/CT using 15-oxygen labeled water as tracer against the validated multiparametric MRI technique.

Once validated the imaging technique will be beneficial in examining vascular damage in the kidneys caused by different renal diseases.

This is a randomized, crossover study where each participant will participate in 1 study day.

Ten healthy male participants in the age group 20-60 years are screened and randomized, and expected to complete the present study. Informed consent is obtained before the screening meeting.

All participants consume a controlled diet with fixed salt intake corresponding to a daily intake of 50-70 mmol + 2 mmol/kg sodium for 4 days before the experiment. On the fourth day, 24-hour urine collection will be performed. Throughout the 4 day period, water intake will be ad libitum and physical activity will not be allowed.

Each participant will undergo both PET/CT and MRI separated by around 1.5 hour.

Total renal blood flow and regional renal perfusion are measured repeatedly altogether 5 times with both PET/CT and MRI. Renal oxygenation and oxygen extraction are measured repeatedly 5 times as well, using MRI. The 5 measurements consist of 2 at baseline (rest), 2 during isometric exercise (handgrip) and 1 during recovery (rest).

Isometric muscle exercise is conducted by squeezing a handgrip coupled to a dynamometer. This increases sympathetic nervous activity, leading to preglomerular vasoconstriction.

Conditions

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Kidneys

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Each participant will undergo intervention, which is PET/CT, and control, which is MRI. Each participant will have both intervention and control performed the same day in randomized order.
Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Group randomized to begin with MRI

Group is randomized to begin with MRI and have PET/CT performed later on the same day

Group Type OTHER

MRI

Intervention Type PROCEDURE

MRI will be performed

PET/CT

Intervention Type PROCEDURE

PET/CT will be performed

Group randomized to begin with PET/CT

Group is randomized to begin with PET/CT and have MRI performed later on the same day

Group Type OTHER

MRI

Intervention Type PROCEDURE

MRI will be performed

PET/CT

Intervention Type PROCEDURE

PET/CT will be performed

Interventions

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MRI

MRI will be performed

Intervention Type PROCEDURE

PET/CT

PET/CT will be performed

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Normal health stated through medical questionnaire, examination and blood samples.

Exclusion Criteria

* Immunosuppressive treatment
* Alcohol abuse
* Treatment with glucocorticoids, Glucagon-like peptide-1 (GLP-1) agonists, dipeptidyl peptidase 4 (DPP-4)-inhibitors
* Medical treatment affecting insulin secretion or cardiovascular endpoints
* Liver disease
* Decreased kidney function
* Severe claustrophobia
* Elements incompatible with MR.
Minimum Eligible Age

18 Years

Maximum Eligible Age

60 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Bispebjerg Hospital

OTHER

Sponsor Role lead

Responsible Party

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Ali Asmar

Chief Physician, Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Ali Asmar, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

Bispebjerg Hospital, Department of Clinical Physiology and Nuclear Medicine

Locations

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Bispebjerg Hospital, Department of Clinical Physiology & Nuclear Medicine

Copenhagen, Capital Region, Denmark

Site Status RECRUITING

Countries

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Denmark

Central Contacts

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Peter Sørensen, MD

Role: CONTACT

+4530516279

Ali Asmar, MD, PhD

Role: CONTACT

Facility Contacts

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Peter Sørensen, MD

Role: primary

+4530516279

Ali Asmar, MD, PhD

Role: backup

Other Identifiers

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H-23037460

Identifier Type: -

Identifier Source: org_study_id

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