Application of Nanopore Adaptive Sequencing

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

Get a concise snapshot of the trial, including recruitment status, study phase, enrollment targets, and key timeline milestones.

Recruitment Status

NOT_YET_RECRUITING

Total Enrollment

100 participants

Study Classification

OBSERVATIONAL

Study Start Date

2024-10-01

Study Completion Date

2026-10-30

Brief Summary

Review the sponsor-provided synopsis that highlights what the study is about and why it is being conducted.

Infection post liver transplantation is an important factor in the death in patients. The traditional method of diagnosing infection post liver transplantation is laboratory tests. But the sensitivity and specificity of blood tests is poor. Next-generation sequencing (NGS) has greater detection rate for mycobacterium tuberculosis, anaerobes and fungi and greater sensitivity compare with blood tests. However use of NGS is limited because of the short read-length. Oxford nanopore adaptive sequencing (NAS) method is the Third Revolution in Sequencing Technology. For each 1 Gbp of data, NAS sequencing detected 45 times more microbiome sequences than Nanopore standard sequencing and 2.5 times more than Illumina sequencing. The purpose of this study is to compare NAS with NGS and laboratory tests for the diagnostic rate of infection post liver transplantation.

Related Clinical Trials

Explore similar clinical trials based on study characteristics and research focus.

Pathogenic Metagenomic Next-generation Sequencing to Optimize the Diagnosis of Decompensated Cirrhosis Infection

NCT06039696

Cirrhosis, Liver

RECRUITING

Single-cell Multiomics and Spatiotemporal Omics Analyze the Mechanism of Liver Degenerative Disease

NCT07265544

Liver Neoplasm HBV Infection Non-alcoholic Fatty Liver Disease NAFLD +1 more

RECRUITING

Integrated Diagnostics for Early Diagnosis of Liver Disease

NCT04666402

Non-Alcoholic Fatty Liver Disease Non-alcoholic Steatohepatitis Alcoholic Liver Disease +1 more

RECRUITING

Radiomics for Diagnosing Liver Diseases and Evaluating Progression

NCT03221049

Hepatitis B / Liver Space-occupying Lesions / Patients After Ablation

UNKNOWN

Estimation of Steatosis on Liver Transplants by Intraoperative Spectrometry

NCT05106322

Liver Transplants

RECRUITING

Detailed Description

Dive into the extended narrative that explains the scientific background, objectives, and procedures in greater depth.

Based on the previous work, the investigators found that: 1. NAS has a higher microbial enrichment efficiency and can detect pathogenic information more quickly compared with nanopores normal sequencing (NNS); 2. NAS can detect more specific pathogen fragments with short-time sequencing; 3. NAS can cover more pathogenic genomes than NNS in a short-time; 4. NAS can detect antibiotic resistance information of pathogenic bacteria in clinical samples.

Conditions

See the medical conditions and disease areas that this research is targeting or investigating.

Liver Transplant Infection

Study Design

Understand how the trial is structured, including allocation methods, masking strategies, primary purpose, and other design elements.

Observational Model Type

CASE_ONLY

Study Time Perspective

PROSPECTIVE

Study Groups

Review each arm or cohort in the study, along with the interventions and objectives associated with them.

NAS group

In this group, the investigators will detect the number of pathogen in alveolar lavage fluid, blood and peritoneal drainage by using NAS.

NAS, NGS and Laboratory tests

Intervention Type DIAGNOSTIC_TEST

Using three diffrent detection methods and comparing their efficiency

NGS group

In this group, the investigators will detect the number of pathogen in alveolar lavage fluid, blood and peritoneal drainage by using NGS.

NAS, NGS and Laboratory tests

Intervention Type DIAGNOSTIC_TEST

Using three diffrent detection methods and comparing their efficiency

Lab Tests group

In this group, the investigators will detect the number of pathogen in alveolar lavage fluid, blood and peritoneal drainage by using laboratory tests.

NAS, NGS and Laboratory tests

Intervention Type DIAGNOSTIC_TEST

Using three diffrent detection methods and comparing their efficiency

Interventions

Learn about the drugs, procedures, or behavioral strategies being tested and how they are applied within this trial.

NAS, NGS and Laboratory tests

Using three diffrent detection methods and comparing their efficiency

Intervention Type DIAGNOSTIC_TEST

Eligibility Criteria

Check the participation requirements, including inclusion and exclusion rules, age limits, and whether healthy volunteers are accepted.

Inclusion Criteria

* Accept liver transplantation
* Signing informed consent voluntarily
* Possessing ability to comprehend material information
* Participating this study voluntarily