A Study of Adipose Tissue in Adaptive Responses to Exercise
NCT ID: NCT06053125
Last Updated: 2025-10-23
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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ACTIVE_NOT_RECRUITING
NA
56 participants
INTERVENTIONAL
2023-11-09
2026-10-31
Brief Summary
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Detailed Description
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Immune cell populations and inflammatory signatures will be assessed in subcutaneous abdominal adipose tissue biopsies collected from obese and normal weight young and older adults before, immediately after, and 3 hours after a 30-min bout of cycling exercise at 70% of maximal oxygen consumption (VO2max). Mass spectrometry, Olink targeted inflammation assays, and RNA sequencing will be used for full proteomic and transcriptomic characterization of the adipose tissue secretome (the proteins and molecules secreted from the adipose tissue) and the cargo of extracellular vesicles isolated from plasma and media collected from cultured human adipose tissue explants generated from each time point.
Overall, the primary hypothesis of the proposed work is that a single bout of exercise triggers transient changes in adipose tissue paracrine/endocrine signals and immune cellular composition. The investigators propose that these responses contribute to the beneficial effects of exercise locally and in distal tissues and that the cumulative effects of acute changes in adipose tissue likely contribute to the positive alterations in adipose tissue associated with exercise training.
Conditions
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Study Design
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NON_RANDOMIZED
PARALLEL
BASIC_SCIENCE
NONE
Study Groups
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Older adults with obesity
Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
Adipose Tissue Biopsy
Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise
Exercise
30 minutes of exercise on a cycle ergometer exercise machine
Older adults without obesity
Participants with obesity as determined by the principal investigator and aged 65-85 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
Adipose Tissue Biopsy
Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise
Exercise
30 minutes of exercise on a cycle ergometer exercise machine
Young adults with obesity
Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
Adipose Tissue Biopsy
Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise
Exercise
30 minutes of exercise on a cycle ergometer exercise machine
Young adults without obesity
Participants with obesity as determined by the principal investigator and aged 18-35 years will have an adipose tissue biopsy taken from the abdomen before and after exercise.
Adipose Tissue Biopsy
Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise
Exercise
30 minutes of exercise on a cycle ergometer exercise machine
Interventions
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Adipose Tissue Biopsy
Fat sample collected from abdomen before exercise, immediately after exercise, and 3 hours after exercise
Exercise
30 minutes of exercise on a cycle ergometer exercise machine
Eligibility Criteria
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Inclusion Criteria
* BMI 18.5 kg/m2 - 40 kg/m2
* Weight stable (≥ 3 months)
* Generally healthy
Exclusion Criteria
* Smoking/tobacco use.
* Alcohol/substance abuse.
* Pregnancy and breastfeeding.
* Anemia.
* Abnormal renal function.
* Blood clotting disorders.
* Coronary artery disease.
* Uncontrolled thyroid disease.
* Liver disease.
* Use of medications known to influence the main outcomes of the study.
* Orthopedic problems that may be aggravated by exercise.
* Chronic disease at the discretion of the investigators.
18 Years
85 Years
ALL
Yes
Sponsors
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National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
NIH
Mayo Clinic
OTHER
Responsible Party
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Hawley E. Kunz
Principal Investigator
Principal Investigators
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Hawley Kunz, PhD
Role: PRINCIPAL_INVESTIGATOR
Mayo Clinic
Locations
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Mayo Clinic
Rochester, Minnesota, United States
Countries
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Related Links
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Mayo Clinic Clinical Trials
Other Identifiers
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23-002049
Identifier Type: -
Identifier Source: org_study_id
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