Association Between PM2.5 Exposure and Ferroptosis in Seizures Patients
NCT ID: NCT05758129
Last Updated: 2023-03-07
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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UNKNOWN
60 participants
OBSERVATIONAL
2022-12-01
2023-03-25
Brief Summary
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Participants will be divided into two groups: the control group and the air pollution to detect the biomarkers of the Nrf2- dependent ferroptosis pathway in seizure patients
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Detailed Description
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Methods:
1. The investigators first enrolled 30 low PM2.5 exposure epileptic children and aged-matched high PM2.5 exposure children who were recently diagnosed with epilepsy (\< 2 months)from two cities (Yangzhou and Xuzhou) as the control and air pollution group based on previous PM2.5 records;
2. Recorded the PM2.5 level in two cities every 5 days between 2022.12 and 2023.3 to compare the difference;
3. Interleukin-6 ELISA（IL6） KIT was used to compare the proinflammatory cytokine level in two groups
4. 4 Hydroxynonenal （4HNE） ELISA KIT was used to compare the lipid reactive oxygen species level in two groups
5. Real-Time Quantitative PCR（RT-qPCR） was used to compare messenger RNA (mRNA) expression of the Nrf2-dependent ferroptosis pathway (Nrf2, SLC7A11, GPX4) Detailed Methods protocol
1\. PM2.5 level source: the PM2.5 levels were recorded via the data from the Ministry of Ecology and Environment of the People's Republic of China (air.cnemc.cn:18007/) 2. Sample collection: At the end of this study, the enrolled participant will come to the hospital to collect peripheral Blood (Affiliated Hospital of Jiangnan University and Xuzhou Children's Hospital, respectively), and the blood samples were collected into ethylene diamine tetraacetic acid（EDTA） vacutainer tubes and then divided into leukocytes and plasma for further experiments 3. Interleukin-6 and 4HNE: Plasma IL-6 and 4HNE concentrations were detected by the competitive ELISA method 4. RTqPCR sequence: h-GPX4 forward: 5'-GAGGCAAGACCGAAGTAAACTAC-3' h-GPX4 reverse: 5'-CCGAACTGGTTACACGGGAA-3' h-SLC7A11 forward: 5'-TCTCCAAAGGAGGTTACCTGC-3' h-SLC7A11 reverse: 5'-AGACTCCCCTCAGTAAAGTGAC-3' h-Nrf2 forward5'-ATAGCTGAGCCCAGTATC-3' h-Nrf2 reverse 5'- CATGCACGTGAGTGCTCT-3' h-GAPDH forward: 5'-GGAGCGAGATCCCTCCAAAAT-3' h-GAPDH reverse: 5'-GGCTGTTGTCATACTTCTCATGG-3'
Conditions
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Study Design
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CASE_CONTROL
CROSS_SECTIONAL
Study Groups
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The control group
the invited recently diagnosed with epilepsy (\< 2 months) live in Wuxi, Jiangsu, China (low PM2.5 level based on previous official PM2.5 records)
No interventions assigned to this group
The PM2.5 exposure group
the invited recently diagnosed with epilepsy live in Xuzhou, Jiangsu, China (high PM2.5 level based on previous official PM2.5 records)
No interventions assigned to this group
Eligibility Criteria
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Inclusion Criteria
2. living in this city for more than two years
3. no exposure to any chemistry pollution before
Exclusion Criteria
2. other neurological disorders except for epilepsy;
3. endocrine diseases;
4. cancer
5. receive anti-proinflammatory cytokines therapy
6 Years
18 Years
ALL
No
Sponsors
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Affiliated Hospital of Jiangnan University
OTHER
Responsible Party
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Locations
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Affiliated Hospital of JiangNan University, Department of Pediatrics
Wuxi, Jiangsu, China
Countries
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Other Identifiers
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2022/11/16
Identifier Type: -
Identifier Source: org_study_id
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