Selecting Specific Bio-markers and Researching Mechanisms of Immune Regulation From Inhalation Injury and Traumatic Lung Injury Based on Single Cell Sequencing Technology

NCT ID: NCT05718830

Last Updated: 2023-02-08

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 21 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2021-01-01
• Study Completion Date: 2021-12-31

Brief Summary

The goal of this observational study is to profile the circulating immunological traits of patients with traumatic lung injury (TLI) and inhalation injury (ILI),providing a new direction for the depth research of the pathogenesis, and providing new immune-related targets for diagnosis of the severity of lung injury and treatment.

The investigators performed scRNA-seq of neutrophil and peripheral blood mononuclear cells (PBMCs) from 10 ml fresh circulating blood from 3 patients with TLI and 3 patients with ILI at longitudinal timepoints,as well as peripheral blood from 3 health controls(HCs).

Detailed Description

The investigators performed single-cell transcriptomic sequencing (BD Rhapsody) on neutrophil and PBMCs extracted from the fresh circulating blood from 3 patients with TLI and 3 patients with ILI at longitudinal timepoints,as well as peripheral blood from 3 health controls(HCs). Three time points are picked by clinical conditions of patients with TLI or ILI: day 1 post hospital admission, day 3 post hospital and the day before discharged which matched to acute stage,progressive stage and convalescence.

Conditions

Traumatic Lung Injury; Inhalation Injury

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: PROSPECTIVE

Study Groups

patients with traumatic lung injury (TLI)

No interventions assigned to this group

patients with inhalation injury (ILI)

No interventions assigned to this group

health controls(HCs)

No interventions assigned to this group

Eligibility Criteria

Inclusion Criteria

1. Lung injury caused by burn trauma is confirmed, and the patient is hospitalized within 24 hours after burn or trauma (the clinician can make a definite diagnosis of lung injury based on the comprehensive judgment of the patient's etiology, history, clinical manifestations, blood gas analysis, laboratory tests and imaging);

2. Patients with inhalation injury caused by head, face and neck burns or inhalation injury combined with multiple body burns < 30%;

3. Patients with simple inhalation injury;

4. The normal control group should have no smoking history and be normal population;

5. According to the provisions of Good Clinical Practice(GCP), the informed consent has been obtained and the informed consent has been signed to volunteer for the test.

Exclusion Criteria

1. Basic pulmonary diseases like chronic obstructive pulmonary disease (COPD), asthma and et al;

2. Severe organ dysfunction;

3. Autoimmune diseases;

4. Tumor;

5. Severe infection;

6. Pregnant or nursing women;

7. Fatal or life-threatening events caused by trauma;

8. Any history which may interfere the results or increase the risk of patients judged by researchers.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 90 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Changhai Hospital

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Locations

Department of Burn Surgery, the First Affiliated Hospital of Naval Medical University

Shanghai, Shanghai Municipality, China

Countries

China

Other Identifiers

ChiCTR2200056981

Identifier Type: -

Identifier Source: org_study_id