Biological Maturation and Plyometric Exercise

NCT ID: NCT05442593

Last Updated: 2022-11-04

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 20 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2022-06-27
• Study Completion Date: 2022-07-30

Brief Summary

Plyometric training comprises one of the most widely used training methods in both individual and team sports, and is widely used by coaches as one of the main training in both adults, and children. Plyometric training highly includes the component of eccentric contraction. However, eccentric muscle action, especially when unaccustomed, can lead to exercise-induced muscle damage (EIMD), which is accompanied by increased delayed onset of muscle soreness (DOMS), inflammatory responses, increased levels of muscle proteins into the circulation, oxidative stress, and reduction of muscle function and performance the following days. Although plyometric training is widely used in children and may lead to EIMD, there is limited data regarding the acute effects of plyometric exercise training in children. Additionally, the effect of the biological maturation status of children on EIMD after acute plyometric exercise training has not been investigated. The aim of this study is to examine the effect of biological maturation on EIMD after acute plyometric exercise training in children.

Detailed Description

Plyometric training comprises one of the most widely used training methods in both individual and team sports. Plyometric training has been shown to improve neuromuscular stimulation, jumping ability, muscle strength, flexibility, muscle mass and muscle performance, running speed and muscle power. Therefore, it is widely used by coaches as one of the main training methods, in both adults, and children).

Plyometric training consists of exercises performed through the stretch-shortening cycle of the muscle where the pre-activated muscle is first stretched (eccentric action) followed by the shortening (concentric) action. Therefore, plyometric training highly includes the component of eccentric contraction. However, eccentric muscle action, especially when unaccustomed, can lead to exercise-induced muscle damage (EIMD). EIMD, amongst others, is accompanied by increased delayed onset of muscle soreness (DOMS), inflammatory responses, oxidative stress, increased levels of muscle proteins and collagen into the circulation, and reduction of muscle function and performance.

The effect of acute plyometric training on EIMD, neuromuscular fatigue and performance has been adequately investigated in adults. Indicatively, an acute protocol of plyometric training increased DOMS, blood inflammatory markers, creatine kinase (CK) and lactate dehydrogenase (LDH) activity, while decreased jumping performance for up to 72 hours after the end of the training. In contrast, data regarding the effect of acute plyometric training on the above indices in children are scarce. Additionally, although some data exist on children versus adults, as far as we know, there is no relative data between children with different stages of biological maturation, regardless the common use of plyometric exercises in youth training. However, such data is crucial for both coaches and young athletes to effectively design the training microcycles and incorporate the training components, but also to reduce the risk of injury.

The aim of the present study is to examine the effect of different biological maturation on EIMD, metabolism, neuromuscular fatigue, oxidative stress, and muscle performance after acute plyometric exercise training in children.

According to a preliminary power analysis (probability error: 0.05, power: 0.80, effect size: 0.30), a total sample of 9 participants per group was considered appropriate in order to detect statistically meaningful changes between groups. Thus, twenty healthy male children, aged 8-15 years old, will participate to the study. Written informed consent will be provided by the parents or legal guardians of children after they will be informed about all risks, discomforts, and benefits involved in the study. The procedures will be in accordance with the 1975 Declaration of Helsinki, as revised in 2013. Approval has been received from the bioethics committee of the Department of Physical Education and Sport Science, University of Thessaly.

The study will be performed in a parallel, repeated measures design. The participants will visit the Department's Exercise Physiology laboratory 9 times in total. During the 1st visit, the participants will be examined by a pediatric endocrinologist in order to estimate their biological maturation and be assigned το a condition of either pre-adolescent or adolescent. A familiarization period with the plyometric training protocol and the evaluation procedures with low intensity will be induced (1st, 2nd, 3rd visit). During the next week (4th and 5th visit), fasting blood samples will be collected in order to estimate testosterone levels, CBC, muscle damage, and oxidative stress markers. Participants will be instructed by a dietitian how to record a 7-days diet recalls to ensure that they do not consume to some greater extent nutrients that may affect EIMD, oxidative stress, and fatigue (e.g. antioxidants, amino acids, etc.). Additionally, assessment of body mass, body height, BMI, body composition, sprint performance, jumping performance, isokinetic strength (concentric, isometric, eccentric), aerobic capacity, EIMD (CK) will be performed. After at least 3 days (6th visit), participants will perform the acute bout of plyometric exercise training, that is 8 sets of 10 maximal squat-jumps with a 2-min rest between sets. DOMS will be estimated prior to and post-training, and lactate concentration prior to, and 4 min after the 4th set and 4 min after the 8th set. Neuromuscular fatigue (maximal voluntary isometric contraction, MVIC) estimation will be performed prior to, and 1 hour, 2 hours, and 3 hours post-training. Additionally, DOMS will be estimated at post-, 24 hours, 48 hours, and 72 hours (7th, 8th, and 9th visit) post-training. Sprint and muscle performance, CBC, CK, and oxidative stress indices will also be estimated at 24 hours, 48 hours, and 72 hours post-training.

Conditions

Biological Maturation

Study Design

• Allocation Method: NON_RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: SCREENING
• Blinding Strategy: NONE

Study Groups

Pre-adolescents

Acute plyometric training

Group Type: EXPERIMENTAL

Plyometric training

Intervention Type: OTHER

Participants will perform: 8 sets of 10 maximal countermovement jumps

Adolescents

Acute plyometric training

Group Type: EXPERIMENTAL

Plyometric training

Intervention Type: OTHER

Participants will perform: 8 sets of 10 maximal countermovement jumps

Interventions

Plyometric training

Participants will perform: 8 sets of 10 maximal countermovement jumps

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Normal BMI
• No history of growth irregularities
• No musculoskeletal injuries for at least six months prior to the study
• No use of drugs or ergogenic supplements for at least one month prior to the study
• No participation at exercise with eccentric component for at least three days prior to the study
• No energy drinks consumption before each experimental trial

Exclusion Criteria

• Abnormal BMI
• History of growth irregularities
• Musculoskeletal injuries in the last six months prior to the study
• Use of drugs or ergogenic supplements in the last month prior to the study
• Participation at exercise with eccentric component in the last three days prior to the study
• Energy drinks consumption before each experimental trial

• Minimum Eligible Age: 8 Years
• Maximum Eligible Age: 15 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

University of Thessaly

OTHER

Sponsor Role: lead

Responsible Party

Chariklia K. Deli

Assistant Professor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Chariklia K Deli, PhD

Role: STUDY_CHAIR

University of Thessaly

Locations

Department of Physical Education and Sport Science, Uninersity of Thessaly

Trikala, Thessaly, Greece

Countries

Greece

References

Marginson V, Rowlands AV, Gleeson NP, Eston RG. Comparison of the symptoms of exercise-induced muscle damage after an initial and repeated bout of plyometric exercise in men and boys. J Appl Physiol (1985). 2005 Sep;99(3):1174-81. doi: 10.1152/japplphysiol.01193.2004. Epub 2005 Apr 7.

Reference Type: BACKGROUND

PMID: 15817716 (View on PubMed)

Deli CK, Fatouros IG, Paschalis V, Georgakouli K, Zalavras A, Avloniti A, Koutedakis Y, Jamurtas AZ. A Comparison of Exercise-Induced Muscle Damage Following Maximal Eccentric Contractions in Men and Boys. Pediatr Exerc Sci. 2017 Aug;29(3):316-325. doi: 10.1123/pes.2016-0185. Epub 2017 Feb 6.

Reference Type: BACKGROUND

PMID: 28165870 (View on PubMed)

Asadi A, Ramirez-Campillo R, Arazi H, Saez de Villarreal E. The effects of maturation on jumping ability and sprint adaptations to plyometric training in youth soccer players. J Sports Sci. 2018 Nov;36(21):2405-2411. doi: 10.1080/02640414.2018.1459151. Epub 2018 Apr 3.

Reference Type: BACKGROUND

PMID: 29611771 (View on PubMed)

Other Identifiers

Biol. Matur. - Plyometric Ex.

Identifier Type: -

Identifier Source: org_study_id