Prandial Metabolic Phenotype in Adults

NCT ID: NCT05400733

Last Updated: 2025-10-01

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

NA

Total Enrollment

50 participants

Study Classification

INTERVENTIONAL

Study Start Date

2022-06-24

Study Completion Date

2026-09-30

Brief Summary

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Previous studies suggest that changes in metabolism due to the effects of aging might serve as a potential for therapeutic agents. The investigating lab developed a pulse method combining multiple stable isotopes to study multiple metabolic pathways in the same individual simultaneously. By measuring whole-body metabolism in a large group of older adults, the investigators may be able to unravel age-related deterioration in protein turnover (synthesis and breakdown) that contributes to impaired functional capacity. Metabolic profiles could explain differences in pathways in the aging process with special interest in metabolism of amino acids as they have been associated with aging-related disorders.

Detailed Description

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The study involves 1 screening visits of approximately 3 hours and 6 study days of approximately 8 hours.

Subjects will be asked to arrive in the fasted state on all study days. Fasting prior to screening is not required. On the screening day, body weight, height, and body composition by Dual-energy X-ray absorptiometry (DXA) will be measured. In addition, The Six-Minute Walk Test (6MWT) and skeletal muscle function tests will be assessed at the end of each screening visit.

Each study visit will begin with vital signs. Before administration of the feeding or the tracer solution, baseline blood will be collected for measurement of the natural enrichment of metabolites. After the baseline sample is collected, feeding will begin and will be followed by tracers of several compounds that may be administered by IV pulse.

Next, the subjects will be fed one of six liquid nutritional supplement every 20 minutes for 6 hours. The liquid nutrition supplement will target the amino acid formulation in the following meals (1) total amino acid, (2) Essential Amino Acids (EAA), (3) EAA + -hydroxy -methylbutyric acid (HMB), (4) EAA + leucine (LEU), (5) EAA high, and (6) Placebo.

Conditions

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Protein Metabolism

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

OTHER

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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Healthy male older adults

Healthy male older adults

Group Type OTHER

Total Amino Acids

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, Alanine, Arginine, Aspartate, Cystine, Glutamine, Glutamate, Glycine, Proline, Serine, Tyrosine. All supplements are commercially available.

Essential Amino Acids

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine. All supplements are commercially available.

Essential Amino Acids + Hydroxy-methylbutyric acid (HMB)

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, HMB. All supplements are commercially available.

Essential Amino Acids + Leucine

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, Extra leucine. All supplements are commercially available.

Essential Amino Acids High

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine. All supplements are commercially available.

Placebo Water

Intervention Type DIETARY_SUPPLEMENT

Water

Healthy female older adults

Healthy female older adults

Group Type OTHER

Total Amino Acids

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, Alanine, Arginine, Aspartate, Cystine, Glutamine, Glutamate, Glycine, Proline, Serine, Tyrosine. All supplements are commercially available.

Essential Amino Acids

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine. All supplements are commercially available.

Essential Amino Acids + Hydroxy-methylbutyric acid (HMB)

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, HMB. All supplements are commercially available.

Essential Amino Acids + Leucine

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, Extra leucine. All supplements are commercially available.

Essential Amino Acids High

Intervention Type DIETARY_SUPPLEMENT

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine. All supplements are commercially available.

Placebo Water

Intervention Type DIETARY_SUPPLEMENT

Water

Interventions

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Total Amino Acids

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, Alanine, Arginine, Aspartate, Cystine, Glutamine, Glutamate, Glycine, Proline, Serine, Tyrosine. All supplements are commercially available.

Intervention Type DIETARY_SUPPLEMENT

Essential Amino Acids

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine. All supplements are commercially available.

Intervention Type DIETARY_SUPPLEMENT

Essential Amino Acids + Hydroxy-methylbutyric acid (HMB)

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, HMB. All supplements are commercially available.

Intervention Type DIETARY_SUPPLEMENT

Essential Amino Acids + Leucine

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine, Extra leucine. All supplements are commercially available.

Intervention Type DIETARY_SUPPLEMENT

Essential Amino Acids High

Histidine, Isoleucine, Leucine, Lysine, Methionine, Phenylalanine, Threonine, Tryptophan, Valine. All supplements are commercially available.

Intervention Type DIETARY_SUPPLEMENT

Placebo Water

Water

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Age: 60-80, inclusive
* Stable body-weight (± 5%) for the past 3 months
* Subject is judged to be in satisfactory health based on medical history, physical examination, and laboratory screening evaluations.
* Ability to walk, sit down and stand up independently or with walking mobility aids
* Ability to lie in supine or elevated position for up to 7 hours
* Willingness and ability to comply with the protocol

Exclusion Criteria

* Established diagnosis of current malignancy
* History of untreated metabolic diseases including hepatic or renal disorder
* Presence of acute illness or metabolically unstable chronic illness
* Presence of fever within the last 3 days
* Use of short course of oral corticosteroids within 4 weeks preceding study day
* (Possible) pregnancy
* Failure to give informed consent or Investigator's uncertainty about the willingness or ability of the subject to comply with the protocol requirements
* Already enrolled in another clinical trial and that clinical trial interferes with participating in this study
* Any other condition according to the PI or nurse that was found during the screening visit, that would interfere with the study or safety of the patient
Minimum Eligible Age

60 Years

Maximum Eligible Age

80 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Texas A&M University

OTHER

Sponsor Role lead

Responsible Party

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Marielle PKJ Engelen, PhD

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Locations

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Human Clinical Research Facility

College Station, Texas, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Marielle Engelen

Role: CONTACT

9792202282

Savanah Knezek

Role: CONTACT

9793186010

Facility Contacts

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Marielle Engelen, Ph.D.

Role: primary

Other Identifiers

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83 - Pulse Feeding

Identifier Type: -

Identifier Source: org_study_id

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