Metabolic Signatures of Dairy Products Ingestion in Humans: Effect of Age

NCT ID: NCT03500003

Last Updated: 2019-08-07

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

28 participants

Study Classification

INTERVENTIONAL

Study Start Date

2018-06-01

Study Completion Date

2019-08-06

Brief Summary

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Despite the fact that dairy products are greatly consumed, it remains today very difficult to determine with a classical blood biochemical test whether a person has ingested a fresh fermented dairy product or just milk, while their biological, metabolic and health impacts may be different.

The metabolic footprint left by the consumption of these products could be modified by the age of the consumer. Indeed, in the elderly, the processes of digestion, assimilation and metabolism are known to be altered /different compared to healthy adults. Thus, different metabolic signatures in the elderly could appear as the result of a less effective use and metabolism of the nutrients ingested. This could further also result in different or altered biological effects on dairy products.

Detailed Description

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The two main objectives of this project are to:

* Determine precisely the plasma and urine metabolic signatures resulting from acute (postprandial) ingestion of yogurt or milk.
* Characterize the metabolic signature following the consumption of these dairy products in the elderly and compared with the signature of reference (adult).
* Understand the mechanisms and metabolic pathways involved if discriminating metabolites between age are detected..

The clinical study is open, monocentric, controlled and randomized, in a cross experimental design.

The study is not performed in a double blind way. However, the measure bias will be limited because of the standardized and objective characteristic of the main criteria. Moreover, biological samples will be analyzed by the same partners of the project. Each subject will be its own control, so that confusion factors related to individual variability will be eliminated.

A total of 28 volunteers (men) will participate in the study: 14 adults and 14 old people (\>65 yrs-old). After 3 weeks of controlled diet (without consumption of dairy products) the volunteers will ingest 600g of milk or yogurt. After 1 week of wash-out, the other product will be consumed. Blood and urine will be collected during the postprandial period.

The monocentric characteristic of the study, the low number of subjects and the expertise of the involved staff will enable to limit the number of missing data.

The randomization (latin square) has been established by a bio-statistician of the project before the study started. A document describing the randomization proceeding is confidentially kept.

Conditions

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Nutrition

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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Milk acute intake

14 adult and 14 elderly volunteers will consume 600mL of milk. Blood (11 sampling points) and urine samples (3 collection points) will be collected before the ingestion and during the postprandial period (6h).

Group Type EXPERIMENTAL

Acute milk intake

Intervention Type OTHER

Food matrix: milk

Yogurt acute intake

14 adult and 14 elderly volunteers will consume 600mL of yogurt. Blood (11 sampling points) and urine samples (3 collection points) will be collected before the ingestion and during the postprandial period (6h).

Group Type EXPERIMENTAL

Acute Yogurt intake

Intervention Type OTHER

Food matrix: yogurt

Interventions

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Acute milk intake

Food matrix: milk

Intervention Type OTHER

Acute Yogurt intake

Food matrix: yogurt

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* no pathology and no medical treatment
* BMI \>=21 and \<=30 kg/m²
* normal biological status
* no dislike for diary intake

Exclusion Criteria

* smoking
* no pathology and no medical treatment (no antibiotics in the last 3 months)
* consumption of nutritional supplements several times at week
Minimum Eligible Age

25 Years

Maximum Eligible Age

80 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Centre de Recherche en Nutrition Humaine d'Auvergne

OTHER_GOV

Sponsor Role collaborator

Agroscope Liebefeld-Posieux Research Station ALP

OTHER

Sponsor Role collaborator

Sergio Polakof

OTHER

Sponsor Role lead

Responsible Party

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Sergio Polakof

PhD

Responsibility Role SPONSOR_INVESTIGATOR

Locations

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Centre de Recherche en Nutrition Humaine d'Auvergne

Clermont-Ferrand, , France

Site Status

Countries

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France

Other Identifiers

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FermentoMilk

Identifier Type: -

Identifier Source: org_study_id

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