Exercise and Insulin Signaling in Human Skeletal Muscle

NCT ID: NCT02987491

Last Updated: 2019-08-28

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: NA
• Total Enrollment: 20 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2016-11-30
• Study Completion Date: 2018-06-28

Brief Summary

Obesity is associated with a decrease in skeletal muscle insulin sensitivity. Aerobic exercise can increase insulin sensitivity in the few hours following exercise, however the cellular mechanisms are not completely understood. The current project is to investigate mechanisms of exercise improvements to skeletal muscle insulin sensitivity.

Detailed Description

Study Overview: We are investigating the mechanisms by which exercise improves the response of skeletal muscle to insulin in lean and obese adults. Participants will complete 4 study visits consisting of: 1) screening visit, 2) maximal exercise test visit, and then a randomized order of 3) a resting metabolic study visit, and 4) an exercise metabolic study visit. Metabolic study visits for resting and exercise conditions will be identical, other than remaining sedentary or performing exercise. Skeletal muscle biopsy samples will be collected during resting, immediately post-exercise and during insulin stimulated conditions.

Conditions

Insulin Sensitivity; Obesity; Sedentary Lifestyle

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: CROSSOVER
• Primary Study Purpose: BASIC_SCIENCE
• Blinding Strategy: NONE

Study Groups

Exercise Metabolic Study Day

Participants will perform a single bout of moderate intensity exercise on a cycle ergometer for 60 minutes.

A total of 3 muscle biopsies will be collected throughout the study day. Insulin sensitivity will be measured using a hyperinsulinemic-euglycemic clamp with glucose tracers.

Group Type: EXPERIMENTAL

Exercise

Intervention Type: BEHAVIORAL

Participants will perform 2 metabolic study days of either resting or acute bout of cycling exercise in a randomized cross-over design.

Resting Metabolic Study Day

Participants will rest quietly in bed for 60 minutes and resting energy metabolism will be measured with a ventilated hood.

A total of 3 muscle biopsies will be collected throughout the study day. Insulin sensitivity will be measured using a hyperinsulinemic-euglycemic clamp with glucose tracers.

Group Type: NO_INTERVENTION

No interventions assigned to this group

Interventions

Exercise

Participants will perform 2 metabolic study days of either resting or acute bout of cycling exercise in a randomized cross-over design.

Intervention Type: BEHAVIORAL

Eligibility Criteria

Inclusion Criteria

• Males or Females
• Aged 18-45 years
• Normal weight (body mass index [BMI] 18-26 kg/m2) or obese (BMI 30-46 kg/m2)
• Sedentary (< 1 hour of planned physical activity per week for ≥ 6 months)
• Weight stable (< 2 kg change in body mass for ≥ 6 months)
• Non-smokers (no tobacco or nicotine use for ≥ 1 year)

Exclusion Criteria

• Hypertension (systolic blood pressure >140 mmHg, diastolic blood pressure > 90mmHg)
• Chronic health condition including diabetes, cardiovascular disease, treated hypertension, cancer, anemia, uncontrolled hyper- or hypothyroidism.
• Pregnant, nursing, irregular menses or post-menopausal (if female)
• Hyperglycemia (fasting glucose >126 mg/dl)
• Hypercholesterolemia (fasting LDL>140mg/dl)
• Hemoglobin < 13.0 g/dl (males), < 11.5 g/dl (females)
• Compromised renal function (outside 135-145 mmol/L sodium, 3.5-5.1 mmol/L potassium)
• Lidocaine allergy
• Medications including β-blockers, angiotensin converting enzyme inhibitors, insulin, thiazolidinediones, metformin, sulfonylureas, chronic non-steroidal anti-inflammatory use, anti-coagulant (e.g. warfarin), current antibiotics, opiates, monoamine oxidase inhibitors, benzodiazepines, or others that may impact the study outcomes
• Any physical limitation that prevents a participant from safely completing the exercise test
• Due to the risks associated with the current protocol, individuals with a diminished capacity to consent will be excluded. Similarly, due to the need for constant, accurate participant monitoring during metabolic study activities, participants will need to clearly understand verbal and written English. Participants who cannot clearly understand verbal and written English will be excluded.

• Minimum Eligible Age: 18 Years
• Maximum Eligible Age: 45 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: Yes

Sponsors

Oregon State University

OTHER

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Matthew Robinson, PhD

Role: PRINCIPAL_INVESTIGATOR

Oregon State University

Locations

Oregon State University

Corvallis, Oregon, United States

Countries

United States

References

Newsom SA, Stierwalt HD, Ehrlicher SE, Robinson MM. Substrate-Specific Respiration of Isolated Skeletal Muscle Mitochondria after 1 h of Moderate Cycling in Sedentary Adults. Med Sci Sports Exerc. 2021 Jul 1;53(7):1375-1384. doi: 10.1249/MSS.0000000000002615.

Reference Type: DERIVED

PMID: 34127633 (View on PubMed)

Stierwalt HD, Ehrlicher SE, Robinson MM, Newsom SA. Skeletal Muscle ACSL Isoforms Relate to Measures of Fat Metabolism in Humans. Med Sci Sports Exerc. 2021 Mar 1;53(3):624-632. doi: 10.1249/MSS.0000000000002487.

Reference Type: DERIVED

PMID: 32796254 (View on PubMed)

Provided Documents

Document Type: Informed Consent Form

View Document

Other Identifiers

7605

Identifier Type: -

Identifier Source: org_study_id