Analysis of Transposon Control Pathways in Germinal Cancers of the Testicle

NCT ID: NCT02873793

Last Updated: 2016-08-22

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

15 participants

Study Classification

OBSERVATIONAL

Study Start Date

2015-04-30

Brief Summary

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By analogy with the mouse, the activity of control pathways for transposable elements (TE) in foetal primordial germinal cells could prove to be a determinant for fertility in adulthood in humans. Moreover, defects in the control of transposable elements (TE) could also contribute to the aetiology of germinal testicular cancer, by inducing chromosomal instability and tumorigenesis. In this context, the aim is to analyse a specific type of adult germinal testicular cancers, seminomas. Directly related to our research interest, the histological, transcriptomic and epigenetic features of theses adult tumours are reminiscent of germinal cells normally present in foetuses. The investigator postulates that these similarities could also extend to the biology of transposable elements (TE), and in particular to the initiation of the control of these elements. The management of seminomas requires surgery, orchiectomy, which consists of the total excision of the affected testicles. Using high-resolution high-throughput sequencing techniques, we propose to analyse the control pathways of transposable elements (TE) in tumour samples harvested from surgical pieces in comparison with adjacent healthy tissue. The results obtained in these tumour samples will be compared with those in normal foetal gonad samples.

Detailed Description

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Conditions

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Germinal Cancers of the Testicle

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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7/8 cases pure seminomas

Extraction of total RNA from healthy and tumour tissues

Intervention Type GENETIC

5 cases of non-seminoma

Extraction of total RNA from healthy and tumour tissues

Intervention Type GENETIC

3 cases of composite tumours seminoma/non-seminoma.

Extraction of total RNA from healthy and tumour tissues

Intervention Type GENETIC

Interventions

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Extraction of total RNA from healthy and tumour tissues

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

* men who have provided oral consent
* men with germinal testicular cancer with negative or low serum markers (Alpha Foeto Protein AFP and Human Chorionic Gonadotrophin hCG), indicators of a seminomatous-type tumour
* men who have undergone orchiectomy (total excision of the affected testicle)
* confirmation of the diagnosis of seminomatous or non-seminomatous type by histology of the surgical piece
* absence of hepatitis B or C or infection with human immunodeficiency virus (HIV).

Exclusion Criteria

* men without national health insurance cover
* Adults under guardianship
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Centre Hospitalier Universitaire Dijon

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Locations

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CHU Dijon Bourgogne

Dijon, , France

Site Status RECRUITING

Countries

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France

Central Contacts

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Patricia FAUQUE

Role: CONTACT

03.80.29.50. 31 ext. +33

Facility Contacts

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Patricia FAUQUE

Role: primary

03.80.29.50. 31 ext. +33

Other Identifiers

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FAUQUE 2014

Identifier Type: -

Identifier Source: org_study_id

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