Analysis of Human Genomic DNA in Embryo's Culture Media
NCT ID: NCT02359747
Last Updated: 2021-02-10
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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UNKNOWN
100 participants
OBSERVATIONAL
2020-01-12
2022-12-31
Brief Summary
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Detailed Description
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Double-stranded DNA (dsDNA) amplifiability will be evaluated in embryos culture media by quantitative PCR on a multicopy gene target (TBC1D3). Quantification will be made with a standard curve constructed with genomic DNA (with culture media -day 3 and day5-, to take into account media inhibitory effects) TSPY1 amplification will be use to assess the presence of Y chromosome WGA followed by PCR on a single copy gene (MTHFR gene) will be performed on a subset of some samples to monitor the C677T polymorphism (genotyping by sequencing) WGA followed by PCR on a single copy gene (MTHFR) will be also performed on blastocoele fluids
Conditions
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Study Design
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COHORT
RETROSPECTIVE
Study Groups
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In Vitro Fertilization treatment
culture medium discarded after IVF treatment
Polymerase Chain Reaction and Whole Genome Amplification
Polymerase Chain Reaction and Whole Genome Amplification
Interventions
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Polymerase Chain Reaction and Whole Genome Amplification
Polymerase Chain Reaction and Whole Genome Amplification
Eligibility Criteria
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Inclusion Criteria
Exclusion Criteria
18 Years
45 Years
ALL
No
Sponsors
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Cervesi Hospital, Cattolica, Italy
OTHER
Responsible Party
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Palini Simone
Senior Clinica Embryologist Lab Director
Locations
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Cervesi Hospital
Cattolica, , Italy
cervesi Hospital
Cattolica, , Italy
Countries
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Central Contacts
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Facility Contacts
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Other Identifiers
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PGD on culture media is easy
Identifier Type: -
Identifier Source: org_study_id
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