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Study the Effect of Oral Zinc Supplementation on Enzymes of Nitric Oxide Pathway

NCT ID: NCT01684059

Last Updated: 2012-09-12

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

60 participants

Study Classification

OBSERVATIONAL

Study Start Date

2011-07-31

Study Completion Date

2012-07-31

Brief Summary

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The ability of spermatozoa to produce reactive oxygen species (ROS) has been respected since the 1940's. Oxidative stress limits the functional competence of mammalian spermatozoa via lipid peroxidation, the induction of oxidative DNA damage and the formation of protein adducts. Nitric oxide (NO) is a free radical generated from the oxidation of L-arginine to L-citrulline by reduced nicotinamide adenine dinucleotide phosphate (NADPH)-dependent NO synthases. Several studies suggest that the overproduction of this free radical and the subsequent excessive exposure to oxidative conditions have a potential pathogenetic implication, which due to the reduction of sperm motility. The present study was conducted to study the effect of Zn supplementation on the levels of NO synthase and arginase in semen of patients with asthenozoospmia.

Detailed Description

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Previous studies suggest that high concentrations of NO play an injurious consequence on spermatozoa kinetic characteristics. These studies reported that NO may react with superoxide or hydrogen peroxide, resulting in the production of peroxinitrite, hydroxyl radical, or singlet oxygen, which cause oxidation of sperm membrane lipids and thiol proteins. NO also may inhibit cellular respiration by nitro-sylation of heme in mitochondrial enzymes, aconitase, and glyceraldehyde phosphate dehydrogenase, leading to a reduction of adenosine triphosphate and that is due to loss of motility of spermatozoa.

Conditions

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Asthenozoospermia

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

RETROSPECTIVE

Study Groups

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Single group

Single group: each participant receive same intervention (zinc sulfate) throughout study (non-randomized)

zinc sulfate

Intervention Type DIETARY_SUPPLEMENT

every participant took two capsules of zinc sulfate per day for three months (each one 220mg)

Interventions

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zinc sulfate

every participant took two capsules of zinc sulfate per day for three months (each one 220mg)

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* the presence of asthenozoospermia in the semen sample.

Exclusion Criteria

* the absence of endocrinopathy,
* varicocele, and
* female factor infertility. Smokers and alcoholic men were excluded from the study because of their recognized high seminal ROS levels and decreased antioxidant levels
Minimum Eligible Age

27 Years

Maximum Eligible Age

35 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Ministry of Health, Iraq

NETWORK

Sponsor Role collaborator

Babylon University

OTHER

Sponsor Role lead

Responsible Party

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mahmoud hussein hadwan

phD

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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mahmoud H. hadwan, phD

Role: PRINCIPAL_INVESTIGATOR

babylon university / Iraq

Lamia A. Almashhedy, phD

Role: STUDY_CHAIR

Babylon university/Iraq

abdulrrazaq S. Alsalman, phD

Role: STUDY_DIRECTOR

Babylon university/Iraq

Locations

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Babylon university/ college of science

Hillah, Muḩāfaz̧at Bābil, Iraq

Site Status

Countries

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Iraq

References

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Hadwan MH, Almashhedy LA, Alsalman AR. Study of the effects of oral zinc supplementation on peroxynitrite levels, arginase activity and NO synthase activity in seminal plasma of Iraqi asthenospermic patients. Reprod Biol Endocrinol. 2014 Jan 3;12:1. doi: 10.1186/1477-7827-12-1.

Reference Type DERIVED
PMID: 24383664 (View on PubMed)

Other Identifiers

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Babil-2

Identifier Type: -

Identifier Source: org_study_id