Impact of Sperm DNA Integrity on In Vitro Cycles

NCT ID: NCT01232465

Last Updated: 2014-04-11

Study Results

Results available

Outcome measurements, participant flow, baseline characteristics, and adverse events have been published for this study.

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Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

420 participants

Study Classification

OBSERVATIONAL

Study Start Date

2010-10-31

Study Completion Date

2013-09-30

Brief Summary

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Sperm DNA integrity will be measured via the Toluidine Blue Assay (TBA) for patients undergoing In Vitro Fertilization (IVF); the study will determine correlations, if any, between IVF success and sperm DNA integrity.

Detailed Description

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Conditions

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Male Infertility Unexplained Infertility Abortion, Habitual

Study Design

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Observational Model Type

CASE_CONTROL

Study Time Perspective

CROSS_SECTIONAL

Study Groups

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IVF

those individuals undergoing conventional IVF to inseminate their retrieved eggs

No interventions assigned to this group

ICSI

those individuals whose eggs were fertilized via intracytoplasmic sperm injection (ICSI)

No interventions assigned to this group

Eligibility Criteria

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Inclusion Criteria

* couple undergoing IVF at the Center for Human Reproduction
* sperm sample given from partner (not a donor)

Exclusion Criteria

* low sperm concentration (\<5 million/cc)
* using a donor sample
Eligible Sex

MALE

Accepts Healthy Volunteers

No

Sponsors

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Northwell Health

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Avner E Hershlag, MD

Role: PRINCIPAL_INVESTIGATOR

Center for Human Reproduction

Locations

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Center for Human Reproduction

Manhasset, New York, United States

Site Status

Countries

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United States

References

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Andreetta AM, Stockert JC, Barrera C. A simple method to detect sperm chromatin abnormalities: cytochemical mechanism and possible value in predicting semen quality in assisted reproductive procedures. Int J Androl. 1995 Jun;18 Suppl 1:23-8. doi: 10.1111/j.1365-2605.1995.tb00634.x.

Reference Type BACKGROUND
PMID: 7558384 (View on PubMed)

Erenpreisa J, Erenpreiss J, Freivalds T, Slaidina M, Krampe R, Butikova J, Ivanov A, Pjanova D. Toluidine blue test for sperm DNA integrity and elaboration of image cytometry algorithm. Cytometry A. 2003 Mar;52(1):19-27. doi: 10.1002/cyto.a.10015.

Reference Type BACKGROUND
PMID: 12596248 (View on PubMed)

Erenpreiss J, Bars J, Lipatnikova V, Erenpreisa J, Zalkalns J. Comparative study of cytochemical tests for sperm chromatin integrity. J Androl. 2001 Jan-Feb;22(1):45-53.

Reference Type BACKGROUND
PMID: 11191087 (View on PubMed)

Erenpreiss J, Jepson K, Giwercman A, Tsarev I, Erenpreisa J, Spano M. Toluidine blue cytometry test for sperm DNA conformation: comparison with the flow cytometric sperm chromatin structure and TUNEL assays. Hum Reprod. 2004 Oct;19(10):2277-82. doi: 10.1093/humrep/deh417. Epub 2004 Jul 22.

Reference Type BACKGROUND
PMID: 15271869 (View on PubMed)

Schlegel PN, Paduch DA. Yet another test of sperm chromatin structure. Fertil Steril. 2005 Oct;84(4):854-9. doi: 10.1016/j.fertnstert.2005.04.050.

Reference Type BACKGROUND
PMID: 16213834 (View on PubMed)

Collins JA, Barnhart KT, Schlegel PN. Do sperm DNA integrity tests predict pregnancy with in vitro fertilization? Fertil Steril. 2008 Apr;89(4):823-31. doi: 10.1016/j.fertnstert.2007.04.055. Epub 2007 Jul 20.

Reference Type BACKGROUND
PMID: 17644094 (View on PubMed)

Simon L, Brunborg G, Stevenson M, Lutton D, McManus J, Lewis SE. Clinical significance of sperm DNA damage in assisted reproduction outcome. Hum Reprod. 2010 Jul;25(7):1594-608. doi: 10.1093/humrep/deq103. Epub 2010 May 6.

Reference Type BACKGROUND
PMID: 20447937 (View on PubMed)

Tarozzi N, Bizzaro D, Flamigni C, Borini A. Clinical relevance of sperm DNA damage in assisted reproduction. Reprod Biomed Online. 2007 Jun;14(6):746-57. doi: 10.1016/s1472-6483(10)60678-5.

Reference Type BACKGROUND
PMID: 17579991 (View on PubMed)

Zini A, Libman J. Sperm DNA damage: clinical significance in the era of assisted reproduction. CMAJ. 2006 Aug 29;175(5):495-500. doi: 10.1503/cmaj.060218.

Reference Type BACKGROUND
PMID: 16940270 (View on PubMed)

Sharma RK, Said T, Agarwal A. Sperm DNA damage and its clinical relevance in assessing reproductive outcome. Asian J Androl. 2004 Jun;6(2):139-48.

Reference Type BACKGROUND
PMID: 15154089 (View on PubMed)

Speyer BE, Pizzey AR, Ranieri M, Joshi R, Delhanty JD, Serhal P. Fall in implantation rates following ICSI with sperm with high DNA fragmentation. Hum Reprod. 2010 Jul;25(7):1609-18. doi: 10.1093/humrep/deq116. Epub 2010 May 22.

Reference Type BACKGROUND
PMID: 20495207 (View on PubMed)

Tsarev I, Bungum M, Giwercman A, Erenpreisa J, Ebessen T, Ernst E, Erenpreiss J. Evaluation of male fertility potential by Toluidine Blue test for sperm chromatin structure assessment. Hum Reprod. 2009 Jul;24(7):1569-74. doi: 10.1093/humrep/dep068. Epub 2009 Mar 21.

Reference Type BACKGROUND
PMID: 19304993 (View on PubMed)

Other Identifiers

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10-249A

Identifier Type: -

Identifier Source: org_study_id

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