Subcision With CO₂ Laser or PRP vs Subcision Alone in Atrophic Acne Scars

NCT ID: NCT07199608

Last Updated: 2025-10-03

Basic Information

• Recruitment Status: NOT_YET_RECRUITING
• Clinical Phase: NA
• Total Enrollment: 42 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2025-11-30
• Study Completion Date: 2027-01-31

Brief Summary

Acne vulgaris is an inflammatory disease of pilosebaceous unit ,leading to different types of scars including atrophic, hypertrophic and keloidal. Atrophic scars are the most common type ,affecting about 75-90% of acne scar cases and can be ice pick, rolling or boxcar in morphology . Theses scars may cause significant impact such as depression, anxiety , and reduced quality of life. multiple treatment modalities exist, including lasers ,chemical peels, dermabrasion, microneedling, subcision and platelet rich plasma. Subcision is a well established technique that improves rolling and boxcar scars by releasing fibrotic strands. Fractional co2 laser induces dermal remodeling and collagen neogenesis, while PRP enhances wound healing and collagen induction through growth factors. Combinig subcision with either co2 laser or PRP may enhance treatment efficacy compared to subcision alone.

rational : Subcision alone may provide limited improvement in some patients. Fractional CO₂ laser and PRP are promising adjunctive modalities that could enhance collagen remodeling and improve cosmetic outcomes. Evaluating their combined effect versus subcision alone will help determine the most effective and safe approach for managing atrophic post-acne scars.

aim of work:To compare the efficacy and safety of combined subcision with either fractional CO₂ laser or platelet-rich plasma versus subcision alone in the treatment of facial atrophic post-acne scars.

Detailed Description

Type of the study: Prospective, comparative, split-face study. - Study Setting: The study will be done in Assiut University Hospital ,dermatology department ,after obtaining approval from ethics committee of Faculty of Medicine, Assiut University in Assiut, Egypt.

Sample Size Calculation:

Based on determining the main outcome variable, the estimated minimum required sample size is 42 cases (21 in each group). Sample size was calculated using G*Power software version 3.1.9.7, based on the following assumptions: The main outcome is the difference in mean Goodman and Baron quantitative scar score between two independent groups of patients: those treated with Subcision + PRP and those treated with Subcision + Fractional CO₂.

Based on clinical experience and after reviewing the literature, a large difference was assumed.

The main statistical test is a one-tailed independent t-test to detect the difference between two means.

Effect size = 0.8 Alpha = 0.05 Power = 0.80

All patients will undergo subcision on both sides of the face, patients will be randomly divided into two groups:

group I (21 patients)-subcision combined with platelet rich plasma injection at right side of the face and subcision alone at the other side.

group II (21 patients)-subcision combined with fractional CO2 at right side of the face and subcision alone at the other side .

Each side will be randomly selected by choosing a sealed opaque envelope containing a card labeled with either laser or PRP representing the right split face side treatments.

Each patient will undergo three sessions with one month interval in between. Patient preparation

1. Each patient's entire face will be disinfected with alcohol and cleaned with saline.

2. Anesthesia will be injected subcutaneously with 2% lidocaine

3. A sharp needle inserted at a shallow angle, with the blade facing upward, at the periphery of the scarred area to create a pathway to facilitate the entry of the blunt cannula.

4. Subcision will be performed with an 18-gauge blunt cannula introduced at a 30° angle into the mid to deep dermis, then moved back and forth in a fan-like motion under the scar to release fibrous bands at the dermal or deep dermal, subcutaneous plane until the sound of bands breaking lost, and no resistance felt (endpoint).

5. To establish hemostasis, firm pressure should be applied for at least 5 min.

6. After subcision, the face should be cleaned with alcohol swabs and then sterile saline.

PRP side (group I): Right side of the face will be injected with PRP and the other by saline.

Fractional side (group II): Right side of the face will be treated with a fractional CO2 laser (10,600 nm) using post acne scar mode (DAESHIN ENTERPRISE CO2, LTD. (D.S.E) 105,207,Digital ro,Guro-gu, Seol, Korea).

Post procedure care All patients should be instructed to avoid sunlight exposure and to use sunscreen (SPF ≥ 50).

Cold compresses in first 24h-48h to avoid edema and ecchymosis. Use topical antibiotic ointment as mupirocin or fusidic acid. Analgesics as paracetamol to decrease the pain.

-Research outcome measures:

1. Primary (main):

Goodman & Baron qualitative & quantitative grading systems.

2. Secondary (subsidiary):

Photography, quartile grading scale. dermoscopic evaluation (erythema, pigmentation, photodamage, follicular density) Data entry and analysis will be carried out using SPSS version 21. Descriptive statistics will be performed with frequency and percentages for categorical variables. Mean and standard deviation for normally distributed numerical variables, while median and interquartile measures for non-normally distributed numerical. Then analytic statistics will be done as the chi-square test for comparing independent categorical variables, and McNemar's test. If the data is normally distributed independent t-test, paired t-test, ANOVA, and Pearsons correlations tests will be used. If the data is not normally distributed Mann-Whitney, Kruskal-Wallis, Willcoxon signed-rank, Friedman, and Spearmans correlation tests will be used. Values will be considered significant when P values equal or less than 0.05.

Conditions

Atrophic Acne Scar

Study Design

• Allocation Method: RANDOMIZED
• Intervention Model: PARALLEL
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

subcision alone

Group Type: ACTIVE_COMPARATOR

subcision

Intervention Type: PROCEDURE

patients will undergo subcision procedure alone using 18 gauge blunt cannula to release fibrous bands under atrophic acne scars.this group will serve as control comparator

subcision+fractional co2 laser

Group Type: EXPERIMENTAL

subcision+fractional co2 laser

Intervention Type: PROCEDURE

patients will undergo subcision procedure followed by fractional co2 laser applied to right side of face, the left side will receive subcision alone

subcision+platelet rich plasma

Group Type: EXPERIMENTAL

subcision+PRP

Intervention Type: BIOLOGICAL

patients will undergo subcision procedure followed by intradermal injection of autologous PRP prepared from patients blood on right side.left side will receive subcision alone

Interventions

subcision

patients will undergo subcision procedure alone using 18 gauge blunt cannula to release fibrous bands under atrophic acne scars.this group will serve as control comparator

Intervention Type: PROCEDURE

subcision+fractional co2 laser

patients will undergo subcision procedure followed by fractional co2 laser applied to right side of face, the left side will receive subcision alone

Intervention Type: PROCEDURE

subcision+PRP

patients will undergo subcision procedure followed by intradermal injection of autologous PRP prepared from patients blood on right side.left side will receive subcision alone

Intervention Type: BIOLOGICAL

Eligibility Criteria

Inclusion Criteria

• - Adult patients of both sexes with mild to severe atrophic post-acne scars.

Exclusion Criteria

• - Age < 18 years
• Pregnant or lactating women
• Active inflammatory acne or infection in treatment area
• Premalignant or malignant lesions
• Photosensitive dermatoses
• Coagulation defects or blood disease
• History of keloid scars
• Systemic retinoid use in previous 6 months

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Assiut University

OTHER

Sponsor Role: lead

Responsible Party

Yasmeen Gamal Abdel Halim Ahmed

doctor

Responsibility Role: PRINCIPAL_INVESTIGATOR

Principal Investigators

Azza Mahfouz Abdel Meguid

Role: STUDY_CHAIR

Assiut University

Reham Maher Abd El Gaber

Role: STUDY_DIRECTOR

Assiut University

Locations

Assiut University Hospitals, Dermatology Department

Asyut, Asyut Governorate, Egypt

Countries

Egypt

Central Contacts

Yasmeen Gamal Abdel Halim

Role: CONTACT

ysoo122221@gmail.com

01024164345

Facility Contacts

Yasmeen Gamal Abdel Halim, master

Role: primary

Yasmine.17289582@med.aun.edu.eg

01024164345

References

Tran BQ, Tran TNA, Doan EVL, Nguyen TTP, Nguyen HT. Simultaneous versus sequential fractional CO2 laser and subcision combination for management of post-acne atrophic scars: A split-face comparative study. J Cosmet Dermatol. 2024 Oct;23(10):3210-3221. doi: 10.1111/jocd.16395. Epub 2024 May 21.

Reference Type: BACKGROUND

PMID: 38770894 (View on PubMed)

Abdelwahab AA, Omar GAB, Hamdino M. A combined subcision approach with either fractional CO2 laser (10,600 nm) or cross-linked hyaluronic acid versus subcision alone in atrophic post-acne scar treatment. Lasers Med Sci. 2022 Dec 24;38(1):20. doi: 10.1007/s10103-022-03677-y.

Reference Type: BACKGROUND

PMID: 36564573 (View on PubMed)

Related Links

https://link.springer.com/

Related Info

Other Identifiers

subcision in acne scars

Identifier Type: -

Identifier Source: org_study_id