Optimization of the Isolation Protocol for Extracellular Vesicles (EVs) From Human Follicular Fluid
NCT ID: NCT06952751
Last Updated: 2025-08-15
Study Results
The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.
Basic Information
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RECRUITING
NA
4 participants
INTERVENTIONAL
2025-07-20
2025-12-31
Brief Summary
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Each method has its advantages and disadvantages, and the purity and efficiency may vary between species and different biological fluids. There are only a limited number of studies comparing the various techniques for application in human follicular fluid.
Objective: This study will compare the three most commonly used techniques for isolating EVs: size-based, sedimentation-based (differential ultracentrifugation), and buoyancy-based isolation techniques.
Methodology:
In this prospective study, follicular fluid will be collected from four patients undergoing transvaginal oocyte retrieval following ovarian stimulation at Ghent University Hospital. Patients over 40 years of age or with endometriosis, adenomyosis, or PCOS (polycystic ovarian syndrome) will not be included in this study. During the oocyte retrieval procedure, follicular fluid will be collected from 2-4 oocytes per patient. Since follicular fluid is not used in the patient's treatment (residual material), it does not affect the treatment.
After collection, the three isolation techniques will be applied to each sample. The techniques will be compared in terms of purity (absence of contamination) and efficiency (EV concentration) using transmission electron microscopy, nanoparticle tracking analysis, and western blotting.
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Detailed Description
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Conditions
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Study Design
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NA
SINGLE_GROUP
BASIC_SCIENCE
NONE
Study Groups
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Intervention group
Intervention group
Differential ultracentrifugation
Differential ultracentrifugation
OptiPrep™ density gradient ultracentrifugation
OptiPrep™ density gradient ultracentrifugation
Size-exclusion chromatography
Size-exclusion chromatography
Combined techniques
Combined techniques
Interventions
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Differential ultracentrifugation
Differential ultracentrifugation
OptiPrep™ density gradient ultracentrifugation
OptiPrep™ density gradient ultracentrifugation
Size-exclusion chromatography
Size-exclusion chromatography
Combined techniques
Combined techniques
Eligibility Criteria
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Inclusion Criteria
Exclusion Criteria
* Diagnosis of PCOS (Polycystic Ovary Syndrome)
* Macroscopic blood contamination at oocyte retrieval
18 Years
40 Years
FEMALE
No
Sponsors
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University Ghent
OTHER
University Hospital, Ghent
OTHER
Responsible Party
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Locations
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Ghent University Hospital
Ghent, , Belgium
Countries
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Central Contacts
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Facility Contacts
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Other Identifiers
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G096624N
Identifier Type: OTHER_GRANT
Identifier Source: secondary_id
ONZ-2024-0419
Identifier Type: -
Identifier Source: org_study_id
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