Outcomes of the Embryos of Different Ovarian Follicle Sizes, and the miRNA Detection in Their Blastocyst Culture

NCT ID: NCT05947526

Last Updated: 2023-07-17

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Total Enrollment

55 participants

Study Classification

OBSERVATIONAL

Study Start Date

2023-06-20

Study Completion Date

2023-12-10

Brief Summary

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In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.

Detailed Description

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In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.

Conditions

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Embryo Quality

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Women referring to infertility clinic for IVF treatment

Women who were referred to infertility clinic with complaint of not having baby go through IVF treatment, and having oocyte pick-up procedure with different sizes of ovarian follicles

Oocyte pick-up

Intervention Type PROCEDURE

With the needle, transvaginally USG-guided oocyte pick-up procedure is going to be performed

Interventions

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Oocyte pick-up

With the needle, transvaginally USG-guided oocyte pick-up procedure is going to be performed

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Female patients aged between 20-38 who are planned to have IVF treatment
* On the day of trigger before the oocyte pick-up, patients having at least 5, and 14 at most follicles exceeding 11mm
* Patient having BMI below 30

Exclusion Criteria

* PCO patients
* Patients having endometriosis
* Patients having male factor with severe oligoazoospermia
Minimum Eligible Age

20 Years

Maximum Eligible Age

38 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

Yes

Sponsors

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Acibadem University

OTHER

Sponsor Role lead

Responsible Party

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Nazli Albayrak

Resident, MD

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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NAZLI ALBAYRAK, MD

Role: PRINCIPAL_INVESTIGATOR

Acıbadem University School of Medicine

Locations

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Acibadem University Atakent Hospital

Istanbul, , Turkey (Türkiye)

Site Status RECRUITING

Countries

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Turkey (Türkiye)

Central Contacts

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NAZLI ALBAYRAK, MD

Role: CONTACT

00905054111626

TURGUT AYDIN, MD

Role: CONTACT

00905323722713

Facility Contacts

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NAZLI ALBAYRAK, MD

Role: primary

00905054111626

TURGUT AYDIN, MD

Role: backup

00905323722713

References

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Shapiro BS, Rasouli MA, Verma K, Raman A, Garner FC, Aguirre M, Kaye L, Bedient C. The effect of ovarian follicle size on oocyte and embryology outcomes. Fertil Steril. 2022 Jun;117(6):1170-1176. doi: 10.1016/j.fertnstert.2022.02.017. Epub 2022 Mar 30.

Reference Type BACKGROUND
PMID: 35367061 (View on PubMed)

Mohr-Sasson A, Orvieto R, Blumenfeld S, Axelrod M, Mor-Hadar D, Grin L, Aizer A, Haas J. The association between follicle size and oocyte development as a function of final follicular maturation triggering. Reprod Biomed Online. 2020 Jun;40(6):887-893. doi: 10.1016/j.rbmo.2020.02.005. Epub 2020 Feb 20.

Reference Type BACKGROUND
PMID: 32389425 (View on PubMed)

Costermans NGJ, Soede NM, van Tricht F, Blokland M, Kemp B, Keijer J, Teerds KJ. Follicular fluid steroid profile in sows: relationship to follicle size and oocyte qualitydagger. Biol Reprod. 2020 Mar 13;102(3):740-749. doi: 10.1093/biolre/ioz217.

Reference Type BACKGROUND
PMID: 31786607 (View on PubMed)

Rosen MP, Shen S, Dobson AT, Rinaudo PF, McCulloch CE, Cedars MI. A quantitative assessment of follicle size on oocyte developmental competence. Fertil Steril. 2008 Sep;90(3):684-90. doi: 10.1016/j.fertnstert.2007.02.011. Epub 2008 Feb 4.

Reference Type BACKGROUND
PMID: 18249377 (View on PubMed)

Annes K, Muller DB, Vilela JAP, Valente RS, Caetano DP, Cibin FWS, Milazzotto MP, Mesquita FS, Belaz KRA, Eberlin MN, Sudano MJ. Influence of follicle size on bovine oocyte lipid composition, follicular metabolic and stress markers, embryo development and blastocyst lipid content. Reprod Fertil Dev. 2019 Mar;31(3):462-472. doi: 10.1071/RD18109.

Reference Type BACKGROUND
PMID: 30282571 (View on PubMed)

Orvieto R, Mohr-Sasson A, Blumenfeld S, Nahum R, Aizer A, Haas J. Does a Large (>24 mm) Follicle Yield a Competent Oocyte/Embryo? Gynecol Obstet Invest. 2020;85(5):416-419. doi: 10.1159/000510876. Epub 2020 Sep 23.

Reference Type BACKGROUND
PMID: 32966987 (View on PubMed)

Kasapoglu I, Orhan A, Aslan K, Sen E, Kaya A, Avci B, Uncu G. Are all antral follicles the same? Size of antral follicles as a key predictor for response to controlled ovarian stimulation. J Obstet Gynaecol. 2022 Apr;42(3):461-466. doi: 10.1080/01443615.2021.1910640. Epub 2021 Jun 22.

Reference Type BACKGROUND
PMID: 34155958 (View on PubMed)

Saith RR, Srinivasan A, Michie D, Sargent IL. Relationships between the developmental potential of human in-vitro fertilization embryos and features describing the embryo, oocyte and follicle. Hum Reprod Update. 1998 Mar-Apr;4(2):121-34. doi: 10.1093/humupd/4.2.121.

Reference Type BACKGROUND
PMID: 9683350 (View on PubMed)

Wittmaack FM, Kreger DO, Blasco L, Tureck RW, Mastroianni L Jr, Lessey BA. Effect of follicular size on oocyte retrieval, fertilization, cleavage, and embryo quality in in vitro fertilization cycles: a 6-year data collection. Fertil Steril. 1994 Dec;62(6):1205-10. doi: 10.1016/s0015-0282(16)57186-6.

Reference Type BACKGROUND
PMID: 7957985 (View on PubMed)

Orvieto R, Mohr-Sasson A, Aizer A, Nahum R, Blumenfeld S, Kirshenbaum M, Haas J. Do Follicles of Obese Patients Yield Competent Oocytes/Embryos? Gynecol Obstet Invest. 2020;85(3):290-294. doi: 10.1159/000508226. Epub 2020 Jun 2.

Reference Type BACKGROUND
PMID: 32485714 (View on PubMed)

Other Identifiers

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2023-10/349

Identifier Type: -

Identifier Source: org_study_id

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