Cryosurvival of Embryos From Dysmorphic Oocytes

NCT ID: NCT00521443

Last Updated: 2007-08-27

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Study Classification

OBSERVATIONAL

Brief Summary

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Various morphological abnormalities of human oocytes were reported to detrimentally affect embryo development. We observed development of frozen thawed embryos derived from oocytes with normal or abnormal morphological features to the blastocyst stage. Cytoplasmic abnormalities of the oocytes were found to negatively affect cryosurvival potential of the embryos.

Detailed Description

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Conditions

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Cryopreservation Blastocyst

Study Design

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Observational Model Type

DEFINED_POPULATION

Study Time Perspective

PROSPECTIVE

Study Groups

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Normal

Embryos derived from morphologically normal MII oocytes

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Irregular Shapes

Embryos derived from irregularly shaped oocytes.

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Large PVS

Embryos derived from oocytes with large perivitelline space.

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Dark Zona

Embryos derived from oocytes with dark zona pellucida

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Dark cytoplasm

Embryos derived from oocytes with dark cytoplasm

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Vacuolar cytoplasm

Embryos derived from oocytes with vacuolated cytoplasm

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Central granulation

Embryos derived from oocytes with centrally granulated cytoplasm

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Double extra

Embryos derived from oocytes with double extracytoplasmic abnormalities

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Double combined

Embryos derived from oocytes with any combination of one extracytoplasmic and one cytoplasmic anomaly

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Double cytoplasmic

Embryos derived from oocytes with any two cytoplasmic anomalies

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Triple extra

Embryos derived from oocytes with triple extracytoplasmic anomaly

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Triple combined

Embryos derived from oocytes with triple combined anomalies

Cryopreservation, thawing and blastocyst culture

Intervention Type PROCEDURE

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Interventions

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Cryopreservation, thawing and blastocyst culture

Good quality human embryos were frozen - thawed and cultured to the blastocyst stage.

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Human embryos derived from morphologically normal or abnormal mature oocytes that fertilized normally by microinjection procedure.
* Embryos were obtained from couples who refused to freeze their supernumerary embryos after fresh embryo transfer in an assisted reproduction treatment cycle.

Exclusion Criteria

* Embryos obtained after microinjection to immature oocytes taht were in vitro matured in culture.
* Embryos obtained from abnormally fertilized zygotes.
Minimum Eligible Age

18 Years

Maximum Eligible Age

45 Years

Eligible Sex

FEMALE

Accepts Healthy Volunteers

No

Sponsors

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V.K.V. American Hospital, Istanbul

OTHER

Sponsor Role lead

Principal Investigators

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Basak Balaban, B.Sc.

Role: STUDY_DIRECTOR

Assisted Reproduction Unit of the VKV American Hospital of Istanbul

Locations

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VKV American Hospital

Istanbul, , Turkey (Türkiye)

Site Status

Countries

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Turkey (Türkiye)

References

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Balaban B, Ata B, Isiklar A, Yakin K, Urman B. Severe cytoplasmic abnormalities of the oocyte decrease cryosurvival and subsequent embryonic development of cryopreserved embryos. Hum Reprod. 2008 Aug;23(8):1778-85. doi: 10.1093/humrep/den127. Epub 2008 May 12.

Reference Type DERIVED
PMID: 18477573 (View on PubMed)

Other Identifiers

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AH-05/04

Identifier Type: -

Identifier Source: org_study_id