Efficacy of Ozone Therapy in Reducing Complications Following Impacted Mandibular Third-molar Surgery

NCT ID: NCT06802354

Last Updated: 2025-01-31

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Clinical Phase

PHASE3

Total Enrollment

20 participants

Study Classification

INTERVENTIONAL

Study Start Date

2024-12-01

Study Completion Date

2025-05-31

Brief Summary

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The goal of this clinical trial is to learn if use of ozone works to treat complications after impacted mandibular third molar surgery. It will also learn does ozone has positive effect on wound healing and quality of life.

The main questions it aims to answer are:

* Does ozone reduce incidence of postoperative compliactions such as pain, swelling or reduced mouth opening after impacted mandibular third molar surgery?
* Does ozone improve wound healing after impacted mandibular third molar surgery?
* Does ozone have positive effect on quality of life after impacted mandibular third molar surgery?

Participants will:

* Receive ozone after impacted mandibular third molar surgery.
* Visit the clinic 1st, 3rd, and 7th days after the intervention for checkups and tests.
* Keep a diary of their symptoms.

Detailed Description

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The aims of this study were: (i) to determine the effects of gaseous ozone on pain, swelling, and trismus and healing process measured by cytokine levels, and (ii) to measure patient quality of life after impacted mandibular third molar surgery.

All participants included in the study will be informed about the aims and expected outcomes of the research, the sample collection procedure, and will sign an informed consent form. All participants will be treated according to the principles of good clinical practice and in accordance with the Helsinki Declaration. The Ethics Committee of the Faculty of Medicine provide study approval.

The surgical procedure for each patient will be performed using a standardized surgical technique in two clinical visits (one side per visit).

The surgical area will be disinfected. To ensure pain relief during the procedure, local anesthesia will be applied using blocks of the inferior alveolar and buccal nerves. After elevating the flap, osteotomy will be performed with a carbide drill using an air drill and continuous irrigation with saline solution. Depending on the morphology of the tooth, the third molar will either be extracted or the crown will be separated from the roots (separation). The wound will be primarily closed with individual sutures, and the sutures will be removed on the seventh postoperative day. After the surgical extraction of the third molar belonging the ozone therapy group, gaseous ozone will be applied to the extraction wound. Ozone will be reapplied 3 days after the intervention. After the surgical extraction of the third molar assigned to the sham ozone therapy, ozone will be applied by placing an inactive AV probe at the extraction site. The sham ozone therapy will also be applied 3 days after the surgical intervention. Postoperative instructions will be given to participants regarding oral hygiene maintenance, the use of cold compresses, and dietary recommendations. All participants will be invited for follow-up examinations on the 1st, 3rd and 7th day after the intervention.

Statistical data analysis will be conducted using SPSS version 20.0 (IBM SPSS Statistics for Windows, Version 20.0. Armonk, NY: IBM Corp, USA). Normality testing will be performed, and depending on the results, parametric and/or non-parametric tests will be applied. To compare differences between groups, chi-square tests, independent t-tests, and/or Mann-Whitney tests will be used. To determine differences within groups, paired t-tests and/or Wilcoxon tests will be applied. The correlation between variables will be determined using Pearson or Spearman correlation. A p-value of \<0.05 will be considered statistically significant in all analyses.

Conditions

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Impacted Mandibular Third Molar Extraction

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

SINGLE_GROUP

The study is a 'split-mouth' randomized clinical controlled trial.
Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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Ozone therapy

After the surgical extraction of the impacted mandibular third molar, gaseous ozone will be applied at the extraction site. Ozone will be reapplied 3 days after the intervention, according to the manufacturer's recommendation for wound treatment following tooth extraction.

Group Type EXPERIMENTAL

Ozone

Intervention Type DRUG

In Ozone group, ozone will be applied at the site of the extraction wound for 30 seconds after the surgical extraction of the third molar, according to the manufacturer's recommendation for wound treatment following tooth extraction.

Sham Comparator

After the surgical extraction of the impacted mandibular third molar, a sham ozone therapy will be applied at the extraction site. The sham ozone therapy will also be reapplied 3 days after the surgical intervention.

Group Type SHAM_COMPARATOR

Placebo

Intervention Type DRUG

In the control group, a sham ozone therapy will be applied at the site of the extraction wound for 30 seconds after the surgical extraction of the third molar .

Interventions

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Ozone

In Ozone group, ozone will be applied at the site of the extraction wound for 30 seconds after the surgical extraction of the third molar, according to the manufacturer's recommendation for wound treatment following tooth extraction.

Intervention Type DRUG

Placebo

In the control group, a sham ozone therapy will be applied at the site of the extraction wound for 30 seconds after the surgical extraction of the third molar .

Intervention Type DRUG

Eligibility Criteria

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Inclusion Criteria

* Patients over 18 years
* Patients with bilaterally impacted mandibular third molars classified as impaction index II B
* Patients in good general health (based on the ASA classification as ASA I and ASA II).

Exclusion Criteria

* Contraindications for ozone application (pregnancy, hyperthyroidism, favism, anemia, myasthenia, bleeding disorders, alcohol intoxication, myocardial infarction, pacemaker, allergy to ozone)
* Patients who have used corticosteroid and antibiotic therapy in the last three months prior to inclusion in the study
* Patients with signs of local inflammation in the oral cavity
* Female patients during pregnancy and breastfeeding.
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Jelena Krunic

OTHER

Sponsor Role lead

Responsible Party

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Jelena Krunic

Associate Professor

Responsibility Role SPONSOR_INVESTIGATOR

Locations

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University of East Sarajevo, Faculty of Medicine

Foča, Republika Srpska, Bosnia and Herzegovina

Site Status RECRUITING

Countries

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Bosnia and Herzegovina

Central Contacts

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Jelena Krunic, Professor

Role: CONTACT

+38758210420

Facility Contacts

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Nikola Stojanovic, Professor

Role: primary

+38758211906

References

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Markovic A, Todorovic Lj. Effectiveness of dexamethasone and low-power laser in minimizing oedema after third molar surgery: a clinical trial. Int J Oral Maxillofac Surg. 2007 Mar;36(3):226-9. doi: 10.1016/j.ijom.2006.10.006. Epub 2006 Dec 8.

Reference Type BACKGROUND
PMID: 17157479 (View on PubMed)

Carrillo JS, Calatayud J, Manso FJ, Barberia E, Martinez JM, Donado M. A randomized double-blind clinical trial on the effectiveness of helium-neon laser in the prevention of pain, swelling and trismus after removal of impacted third molars. Int Dent J. 1990 Feb;40(1):31-6.

Reference Type BACKGROUND
PMID: 2407660 (View on PubMed)

Aitken RC. Measurement of feelings using visual analogue scales. Proc R Soc Med. 1969 Oct;62(10):989-93. doi: 10.1177/003591576906201005. No abstract available.

Reference Type BACKGROUND
PMID: 4899510 (View on PubMed)

Carlsson AM. Assessment of chronic pain. I. Aspects of the reliability and validity of the visual analogue scale. Pain. 1983 May;16(1):87-101. doi: 10.1016/0304-3959(83)90088-X.

Reference Type BACKGROUND
PMID: 6602967 (View on PubMed)

34. Pell GJ, Gregory BT. Impacted mandibular third molars: Classification and Impacted mandibular third molars: Classification and modified technique for removal. Dent. Digest. 1933;39:330-8.

Reference Type BACKGROUND

Other Identifiers

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19.032/961-110/23

Identifier Type: OTHER_GRANT

Identifier Source: secondary_id

01-2-9

Identifier Type: -

Identifier Source: org_study_id

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