Impact of Dietary Nitrate Supplementation on VO2max During Ramadan Fasting in Healthy Adults

NCT ID: NCT06767176

Last Updated: 2025-01-13

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

NOT_YET_RECRUITING

Clinical Phase

NA

Total Enrollment

24 participants

Study Classification

INTERVENTIONAL

Study Start Date

2025-01-31

Study Completion Date

2025-03-31

Brief Summary

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The goal of this clinical trial is to determine if beetroot juice supplementation changes exercise performance following one week of Ramadan fasting in young, healthy adults. The main question it aims to answer is:

Does daily beetroot juice supplementation change VO2 max in fasting individuals during the first week of Ramadan?

Participants will:

Visit the lab before the start of Ramadan and after one week of fasting If assigned to intervention group, consume daily shots of beetroot juice (70ml) during the first week of Ramadan fasting Perform maximal exercise testing on a cycle ergometer

Detailed Description

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Recent literature demonstrates data showing a decrease in VO2max during the first week of Ramadan fasting. This is a practice observed by millions around the world every year, where individuals abstain from food and water from sunrise to sunset, dictated by the lunar calendar. Dietary nitrate consumption has been shown to increase VO2max and exercise performance. Therefore, the primary purpose of this study is to investigate whether dietary nitrate, in the form of beetroot juice, can help mitigate the decrease in VO2 max during the first week of Ramadan fasting.

24 young healthy adults will be recruited to undergo these two visits. The first visit will assess maximal oxygen uptake to determine aerobic fitness and metabolic exercise intensity zones under non-fasting conditions. The second visit will be completed after one week of Ramadan fasting. If assigned to the intervention group, participants will consume one shot (70ml) of Blonyx - Beet It Sport Nitrate 400 beetroot juice every day. Each shot contains 400mg of nitrates. Urine Specific Gravity will be measured after each visit to assess hydration status. The intervention group will also undergo Single Blood Draws after each visit to assess nitrate concentrations before and after a week of consumption.

Conditions

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Ramadan Fasting

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

NONE

Study Groups

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No Intervention

Normal Fasting, no intervention. During this arm, participants will be asked to complete an exercise test before and after 1 week of Ramadan fasting.

Group Type NO_INTERVENTION

No interventions assigned to this group

Nitrate Supplementation

Participants will be asked to consume daily nitrate supplementation (70ml) in the form of beetroot juice for 7 days. Participants will then complete a maximal exercise test as they fast.

Group Type EXPERIMENTAL

Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training

Intervention Type DIETARY_SUPPLEMENT

7 doses, taken daily, 70 ml shots, consumed in the morning before the start of fasting

Interventions

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Beetroot Juice (Beet-It Stamina Shot) & Supervised Exercise Training

7 doses, taken daily, 70 ml shots, consumed in the morning before the start of fasting

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Free of known cardiovascular or metabolic diseases or sleep disorders
* No history of smoking (within the past 3 months)
* Able to engage in physical activity assessed through the physical activity readiness questionnaire (PAR-Q+)
* No prescription of chronic medications other than oral contraceptives
* Able to abide by fasting protocols for all visits
* Individuals who are not allergic to ultrasound gel or beetroot juice
* Individuals who are not pregnant

Exclusion Criteria

* Individuals diagnosed with cardiovascular or metabolic disease or sleep disorders
* Has a history of smoking (within the past 3 months)
* Not ready to engage in physical activity as assessed by the PAR-Q+
* Individuals prescribed chronic medications other than oral contraceptives
* Unable to abide by fasting protocols for any testing visit
* Allergic to ultrasound gel or beetroot juice
* Pregnancy
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University of Guelph

OTHER

Sponsor Role lead

Responsible Party

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Philip Millar

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Phillip Millar, PhD

Role: PRINCIPAL_INVESTIGATOR

University of Guelph

Central Contacts

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Mikail Bhimani, MSc

Role: CONTACT

226-235-1099

Julian Bommarito, PhD

Role: CONTACT

9052209829 ext. 54818

Related Links

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Other Identifiers

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25-01-006

Identifier Type: -

Identifier Source: org_study_id

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